F4AWZ6 · F4AWZ6_DOKS4
- ProteinPyruvate carboxylase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1150 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Catalytic activity
- hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H+
Cofactor
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 118 | ATP (UniProtKB | ChEBI) | |||
Binding site | 202 | ATP (UniProtKB | ChEBI) | |||
Binding site | 237 | ATP (UniProtKB | ChEBI) | |||
Active site | 294 | ||||
Binding site | 542 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 614 | substrate | |||
Binding site | 712 | Mn2+ (UniProtKB | ChEBI); via carbamate group | |||
Binding site | 741 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 743 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 876 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | pyruvate carboxylase activity | |
Biological Process | gluconeogenesis | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate carboxylase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacteroidota > Flavobacteriia > Flavobacteriales > Flavobacteriaceae > Dokdonia
Accessions
- Primary accessionF4AWZ6
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 712 | N6-carboxylysine | |||
Modified residue | 1116 | N6-biotinyllysine | |||
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 2-455 | Biotin carboxylation | |||
Domain | 122-319 | ATP-grasp | |||
Domain | 533-802 | Pyruvate carboxyltransferase | |||
Domain | 1075-1150 | Lipoyl-binding | |||
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,150
- Mass (Da)129,141
- Last updated2011-06-28 v1
- Checksum6C9DFB8E412D260E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP002528 EMBL· GenBank· DDBJ | AEE20804.1 EMBL· GenBank· DDBJ | Genomic DNA |