F4AWZ6 · F4AWZ6_DOKS4

Function

function

Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.

Catalytic activity

Cofactor

biotin (UniProtKB | Rhea| CHEBI:57586 )

Pathway

Carbohydrate biosynthesis; gluconeogenesis.

Features

Showing features for binding site, active site.

111501002003004005006007008009001,0001,100
Type
IDPosition(s)Description
Binding site118ATP (UniProtKB | ChEBI)
Binding site202ATP (UniProtKB | ChEBI)
Binding site237ATP (UniProtKB | ChEBI)
Active site294
Binding site542Mn2+ (UniProtKB | ChEBI)
Binding site614substrate
Binding site712Mn2+ (UniProtKB | ChEBI); via carbamate group
Binding site741Mn2+ (UniProtKB | ChEBI)
Binding site743Mn2+ (UniProtKB | ChEBI)
Binding site876substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular Functionpyruvate carboxylase activity
Biological Processgluconeogenesis
Biological Processpyruvate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pyruvate carboxylase
  • EC number

Gene names

    • Ordered locus names
      Krodi_2829

Organism names

Accessions

  • Primary accession
    F4AWZ6

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue712N6-carboxylysine
Modified residue1116N6-biotinyllysine

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain2-455Biotin carboxylation
Domain122-319ATP-grasp
Domain533-802Pyruvate carboxyltransferase
Domain1075-1150Lipoyl-binding

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,150
  • Mass (Da)
    129,141
  • Last updated
    2011-06-28 v1
  • Checksum
    6C9DFB8E412D260E
MKIKKVLVANRGEIAIRVLRACTELNIATVAIYTYEDRYSQHRNKADESYQIGKNNEPLKPYLDMDQIIDLAKSKHVDAIHPGYGFLSENSTFARKCAENGIIFIGPKPEVMDALGDKITAKKVAQQCGVPIIESNKTALDTLDIALSEATKIGYPLMLKAASGGGGRGMRIIRKVEDLQLSFDSARNEALNSFGDDTMFLEKYVEDPKHIEVQIVADSHGNIRHLYERDCSVQRRHQKVVEMAPSYGLSQTLRDNLYKYAVAIATEVNYNNIGTVEFLVDADDNIYFIEVNPRIQVEHTVTEMVTGIDLVKTQIFVAGGYNLSDKQIKIYGQESIGTYGFAIQCRLTTEDPVNNFTPDYGKITTYRSASGMGIRLDAGSIYQGYKVSPFFDSMLVKVSAHGRTLDGSIRKMVRALKEFRIRGVETNIHFLQNVIQTDTFKNGKTTVNFIQNTPALFDIKLSQDRTSKVVQFLAEVSVNGNSDVKKKDDSKIFRTPETPLYSLSDPFPKGTKDLLTELGPEEFCKWLMAEKKIHYTDTTLRDAHQSLLATRMRSYDMLKVASSFAKNHPNTFSMEVWGGATFDVCLRFLHESPWTRLRELRKAVPNILFQMLLRGSNGVGYKAYPDNLIEKFVEKSWENGVDIFRIFDSLNWVKAMEPSIQYVRNLTGGIAEGAISYTGDILDVKQTKYDLKYYTQLAKDLENAGAHMIAIKDMAGLLKPYAATELVTALKDTVNLPIHLHTHDTSSLQTATYLKAIEANVDVVDVALGGLSGLTSQPNFNAVVEMMKYQEREHPFEMDKLNQFSNFWEDTREIYYPFESGLKAGTAEVFEHEIPGGQYSNLRPQAMALGLGDRFAEVKKMYAAVNSMFGNLVKVTPSSKVVGDMAIFMVTNNLTPEDVMERGDTISFPESVINFFKGDLGQPVGGFPKKLQAIILKNKDAYTDRPNAHLAPVDFDKEYETFTAKFQKGFTRPLEMEDFLSYMLYPKVFEQAHENYKKYGNLALVPTKNFFYGMALGEEILIELEPGKTVIIKLLSIGIPSEEGMRTVFFKVNGENRFVEVHDTSLNIKKVENLKVDPEDSNQIGAPLQGSLYKVLVKKGQEVKENDPLFIIEAMKMETTVVASKDGRVASITVSDGNMVSQNDLVLTIE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP002528
EMBL· GenBank· DDBJ
AEE20804.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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