F3YAP5 · F3YAP5_MELPT

Function

function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site, active site.

169350100150200250300350400450500550600650
TypeIDPosition(s)Description
Site34Interaction with DNA
Site140Interaction with DNA
Site141Interaction with DNA
Site144Interaction with DNA
Site149Interaction with DNA
Site156Interaction with DNA
Active site300O-(5'-phospho-DNA)-tyrosine intermediate
Site302Interaction with DNA
Site492Interaction with DNA

GO annotations

AspectTerm
Cellular Componentchromosome
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 1
  • EC number
  • Alternative names
    • DNA topoisomerase I

Gene names

    • Name
      topA
    • Ordered locus names
      MPTP_1121

Organism names

Accessions

  • Primary accession
    F3YAP5

Proteomes

Subcellular Location

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain4-114Toprim
Region164-169Interaction with DNA

Sequence similarities

Belongs to the type IA topoisomerase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    693
  • Mass (Da)
    79,229
  • Last updated
    2011-06-28 v1
  • Checksum
    B7678B6B80AE047D
MAYKYLVIVESPAKAKTIEKYLGRNYKVVASVGHIRDLPKSKMGIDIENDYAPHYISIRGKGDVIKSLKTAAKKADKVYLAADPDREGEAIAWHLSYLLGLDPNEKNRVVFNEITKEAVKAAFKEPRTINLDLVNAQQARRILDRLVGYSISPILWRKVKKGLSAGRVQSVALKIIIDREKEIKEFIPEEYWTIDGNFQKGRKKFKASFWGIEGKKKKLPKADSVKEITDKLKDNKYQVTKVEKKERKRNPALPFTTSSLQQEAARKLNFRTRKIMMIAQQLYEGISLGKKQGTVGLITYMRTDSTRISASAKVEATEYIEQTYGKEFSIQEERKVKKAQGTQDAHEAVRPSSVLRTPDSIKEYLNKDQLKLYTLIWSRFLASQMAPALLDTMKVTLEQNQVMFIANGSKIKFKGFMQVYIEGRDDGKEEKENILPELVEGDSVTSINIEPKQHFTQPPARFSEAALIHNLEENGVGRPSTYAPTLETIQKRYYVKLTNKRFEPTELGEIINTLIVDFFPQIVDVHFTAAMEEDLDKIGDGKEEWITVVDRFYQPFEKELTNAEEKIEKIQIKDEPAGFSCEECDHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVRCPICHEGQVIERKSKKNKLFYGCSRYPDCDFTSWDKPVGRLCPKCGKYLVEKKVKGGKQVVCINGDYEENVQK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP012200
EMBL· GenBank· DDBJ
BAK21573.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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