F3YAP4 · F3YAP4_MELPT
- ProteinMethylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
- GenetrmFO
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids436 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.
Catalytic activity
- (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H+ + NADH + uridine54 in tRNA = (6S)-5,6,7,8-tetrahydrofolate + 5-methyluridine54 in tRNA + NAD+
CHEBI:15636 + CHEBI:15378 + CHEBI:57945 + RHEA-COMP:10193 CHEBI:65315 Position: 54= CHEBI:57453 + RHEA-COMP:10167 CHEBI:74447 Position: 54+ CHEBI:57540
Cofactor
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | tRNA (uracil(54)-C5)-methyltransferase activity, 5,10-methylenetetrahydrofolate-dependent | |
Biological Process | tRNA methylation | |
Biological Process | tRNA wobble uridine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMethylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Enterococcaceae > Melissococcus
Accessions
- Primary accessionF3YAP4
Proteomes
Subcellular Location
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 5-365 | MnmG N-terminal | ||||
Sequence: VKVIGAGLAGSEAAWQIANNGVAVELYEMRPNKQTPAHQTDHFAELVCSNSLRGNSLTNAVGVLKEEMRQLNSLIIKSADETAVPAGGALAVDRENFSRMVTEKIKKHPLITIKKEEVTNIPNDNTVIATGPLTTECLSEEIKCFNDSEGFYFYDAAAPIVDQSTINMEKVYLKSRYNKGEAAYLNCPMTQEEFQAFYEALIAAETVPLKTFEKEKYFEGCMPIEVMAKRGIKTMLFGPMKPVGLEDPKTGKRPFAVIQLRQDNAAASLYNMVGFQTHLKWGEQKRVFQMIPGLENAEFIRYGVMHRNSFINSPELLKPTYQSQKRDNLFFAGQMTGVEGYVESAASGLLAGINAARLTKN |
Sequence similarities
Belongs to the MnmG family. TrmFO subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length436
- Mass (Da)48,777
- Last updated2011-06-28 v1
- Checksum3F5AE3CE44DB4A89
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP012200 EMBL· GenBank· DDBJ | BAK21572.1 EMBL· GenBank· DDBJ | Genomic DNA |