F2NRL2 · F2NRL2_TRES6

Function

function

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site12[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site45(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site45dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site45isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site80(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site80dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site80isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site102[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site130(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site130dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site130isopentenyl diphosphate (UniProtKB | ChEBI)
Active site132Proton donor
Binding site168(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site196[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site224(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site224dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site224isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site226(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site226dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site226isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site267(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site267dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site267isopentenyl diphosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Molecular Functionmetal ion binding
Biological Processdimethylallyl diphosphate biosynthetic process
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • EC number
  • Short names
    HMBPP reductase

Gene names

    • Name
      ispH
    • Ordered locus names
      Tresu_0906

Organism names

Accessions

  • Primary accession
    F2NRL2

Proteomes

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the IspH family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    284
  • Mass (Da)
    30,460
  • Last updated
    2011-05-31 v1
  • MD5 Checksum
    A785D91E2C965818FC6FED0745AE7D83
MKVVRAKVLGFCMGVRRAVETASAALENSEKNGHKKVFTLGPLIHNPLVMDSLEKKGAQILEPSSLEKAKSGSVVVIRAHGTTPQVVSALENQGAEILDATCPRVHLSQKRAAEWKKKGFLVVIAGDKNHGEVVSISGYAENHAVVVESAEEALKIEIPEKTVLIAQTTFSPVEFEKIASALKQKNPSVQIFNSICSATMERQNALKELEGKVDAILVIGGKNSANTRRLFETASLICGKTALIEDSSEIPDSFFSLKTVGLTAGASTPDFVIDEIERALLSGK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP002631
EMBL· GenBank· DDBJ
AEB13830.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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