F2J290 · F2J290_POLGS

Function

function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.
    EC:6.5.1.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site46-50NAD+ (UniProtKB | ChEBI)
Binding site95-96NAD+ (UniProtKB | ChEBI)
Binding site130NAD+ (UniProtKB | ChEBI)
Active site132N6-AMP-lysine intermediate
Binding site153NAD+ (UniProtKB | ChEBI)
Binding site189NAD+ (UniProtKB | ChEBI)
Binding site305NAD+ (UniProtKB | ChEBI)
Binding site329NAD+ (UniProtKB | ChEBI)
Binding site434Zn2+ (UniProtKB | ChEBI)
Binding site437Zn2+ (UniProtKB | ChEBI)
Binding site464Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionDNA ligase (NAD+) activity
Molecular Functionmetal ion binding
Biological Processbase-excision repair, DNA ligation
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA ligase
  • EC number
  • Alternative names
    • Polydeoxyribonucleotide synthase [NAD(+)]

Gene names

    • Name
      ligA
    • Ordered locus names
      SL003B_1358

Organism names

Accessions

  • Primary accession
    F2J290

Proteomes

Subcellular Location

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain643-716BRCT

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    721
  • Mass (Da)
    77,656
  • Last updated
    2011-05-31 v1
  • Checksum
    D66BECB00DB35C42
MNEKSAAAAVESLSRQEAEAELARLAAEIAAHDQRYFAEDAPTVSDAEYDALRRRNAAIEERFPDLVREDSPSARIGAAPSEGFGKVTHAIPMLSLDNAFSDDDVRDFVARVRRFLRLDPLMGALGVTAEPKIDGLSLSLRYEGGRLVSAATRGDGTVGENVTANARTIADIPHRLPAGVPEVVEVRGEVYMAHADFAALNARMAANGGKVFANPRNAAAGSLRQLNPDITAARPLKFFAYAWGEMSEVPRATQMEMVSLIGAWGFPVNPRMVLCRSVEELIDVYHAIEEERATLGYDIDGVVYKVDRLDLQARLGFVSRSPRWAIAHKFPAEKAFTILRDIEIQVGRTGALTPVAKLEPVTVGGVVVSNATLHNEDYIRGIGQDGEPIRGGKDLRIGDTVQIQRAGDVIPQVIDVDLAKRPADAVPFAFPTVCPVCGSHAVREINAKTGRPDAVRRCTGGLVCAAQAKEKLKHFVSRNAFDIEGLGDKQVEFFYDLPPEEGGIRTPADIFTLEARDAASPIRKLKNRDGWGETSARNLFAAIDARRRVDLNRFIFALGIRHVGEGNAKLLARAYGSWDAFAAAMRAAADHGSDAWAELNAIDGVGEVVAEAVVQFFAEPHNREALDALLAEVTPLDAEKVAAEGSPVAGKTVVFTGALERMTRDEAKAMAERLGAKVAGSVSKKTSLVVAGPGAGSKLKTAQELDIEVITEDDWFDLVGG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP002568
EMBL· GenBank· DDBJ
ADZ69786.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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