F2F738 · F2F738_SOLSS
- ProteinPyruvate carboxylase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1144 (go to sequence)
- Protein existencePredicted
- Annotation score2/5
Function
function
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Catalytic activity
- hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H+
Cofactor
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 119 | ATP (UniProtKB | ChEBI) | |||
Binding site | 203 | ATP (UniProtKB | ChEBI) | |||
Binding site | 238 | ATP (UniProtKB | ChEBI) | |||
Active site | 294 | ||||
Binding site | 541 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 613 | substrate | |||
Binding site | 710 | Mn2+ (UniProtKB | ChEBI); via carbamate group | |||
Binding site | 739 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 741 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 874 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | pyruvate carboxylase activity | |
Biological Process | gluconeogenesis | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate carboxylase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillati > Bacillota > Bacilli > Bacillales > Planococcaceae > Solibacillus
Accessions
- Primary accessionF2F738
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 710 | N6-carboxylysine | |||
Modified residue | 1110 | N6-biotinyllysine | |||
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 3-455 | Biotin carboxylation | |||
Domain | 125-319 | ATP-grasp | |||
Domain | 532-800 | Pyruvate carboxyltransferase | |||
Domain | 1069-1144 | Lipoyl-binding | |||
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,144
- Mass (Da)127,292
- Last updated2011-05-31 v1
- MD5 Checksum998E9AE3BF5DBBC7F70497CFD619995A
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP012157 EMBL· GenBank· DDBJ | BAK17557.1 EMBL· GenBank· DDBJ | Genomic DNA |