F2CS69 · F2CS69_HORVV

Function

function

Catalytic subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase. Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

Allosterically activated by fructose 2,6-bisphosphate.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Features

Showing features for binding site, site, active site.

TypeIDPosition(s)Description
Binding site98diphosphate (UniProtKB | ChEBI)
Binding site192Mg2+ (UniProtKB | ChEBI); catalytic
Site193Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATP
Site219Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPi
Binding site220-222substrate
Active site222Proton acceptor
Binding site259-260substrate; ligand shared between dimeric partners
Binding site267-269substrate
Binding site328substrate
Binding site433-436substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function6-phosphofructokinase activity
Molecular FunctionATP binding
Molecular Functiondiphosphate-fructose-6-phosphate 1-phosphotransferase activity
Molecular Functionmetal ion binding
Biological Processfructose 6-phosphate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta
  • EC number
  • Short names
    PFP
  • Alternative names
    • 6-phosphofructokinase, pyrophosphate dependent
    • PPi-PFK
    • Pyrophosphate-dependent 6-phosphofructose-1-kinase

Gene names

    • Name
      PFP-BETA

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Morex
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Pooideae > Triticodae > Triticeae > Hordeinae > Hordeum

Accessions

  • Primary accession
    F2CS69

Proteomes

Genome annotation databases

Subcellular Location

Keywords

Interaction

Subunit

Tetramer of two alpha (regulatory) and two beta (catalytic) chains.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain90-343Phosphofructokinase

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    560
  • Mass (Da)
    60,621
  • Last updated
    2011-05-31 v1
  • Checksum
    D46A75FEF8A4B0AB
MAAAAVASNGGAAPVPGRLASVYSEVQTSRIAHMLPLPSVLRSHFTLADGPASTAAGSPDEIAKLFPCLYGQPSASVVPTAEPVASKPLKIGVVLSGGQAPGGHNVICGIFDYLQERAKGSTMYGFKGGPAGVMKGKYVELTADFVYPYRNQGGFDMICSGRDKIETPEQFKQAEDTVNRLDLDGLVVIGGDDSNTNACLLGEYFRGRNLKTRVIGCPKTIDGDLKCKEVPTSFGFDTACKIYSEMIGNVMTDARSTGKYYHFVRLMGRAASHITLECALQTHPNVALIGEEVAEKKETLKNVTDYITDVVCKRAELGYNYGVVLIPEGLIDFIPEIQKLIAELNEILAHDVVDEAGAWKSKLEPASRQLFDFLPKTIQEQLLLERDPHGNVQVAKIETEKMLIAMVETELEKRRAAGKYSAHFRGQSHFFGYEGRCGLPTNFDSSYCYALGYGAGALLQFGKTGLISSVGNLAAPVEQWTVGGTALTALMDVERRHGKFKPVIKKAMVELDAAPFKKFASMRDEWAIKNRYISPGPIQFSGPGSDASNHTLMLELGAEI

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AK354471
EMBL· GenBank· DDBJ
BAJ85690.1
EMBL· GenBank· DDBJ
mRNA
AK354615
EMBL· GenBank· DDBJ
BAJ85834.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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