F1YDU7 · F1YDU7_9ACTN

Function

function

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per monomer.

Features

Showing features for binding site.

138450100150200250300350
TypeIDPosition(s)Description
Binding site145Zn2+ 1 (UniProtKB | ChEBI)
Binding site148Zn2+ 1 (UniProtKB | ChEBI)
Binding site162Zn2+ 2 (UniProtKB | ChEBI)
Binding site165Zn2+ 2 (UniProtKB | ChEBI)
Binding site188Zn2+ 2 (UniProtKB | ChEBI)
Binding site191Zn2+ 2 (UniProtKB | ChEBI)
Binding site202Zn2+ 1 (UniProtKB | ChEBI)
Binding site205Zn2+ 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionheat shock protein binding
Molecular Functionunfolded protein binding
Molecular Functionzinc ion binding
Biological Processchaperone cofactor-dependent protein refolding
Biological ProcessDNA replication
Biological Processprotein refolding
Biological Processresponse to heat

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Chaperone protein DnaJ

Gene names

    • Name
      dnaJ
    • ORF names
      SCNU_01635

Organism names

Accessions

  • Primary accession
    F1YDU7

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, zinc finger, repeat.

TypeIDPosition(s)Description
Domain4-66J
Domain132-214CR-type
Zinc finger132-214CR-type
Repeat145-152CXXCXGXG motif
Repeat162-169CXXCXGXG motif
Repeat188-195CXXCXGXG motif
Repeat202-209CXXCXGXG motif

Domain

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.

Sequence similarities

Belongs to the DnaJ family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    384
  • Mass (Da)
    39,765
  • Last updated
    2011-05-31 v1
  • Checksum
    E72C12A95ACCE438
MARDYYGILGVSAGASDQEIKRAYRKMARELHPDVNPDAEDQFKEVTAAYEVLSDPEKRRIVDAGGDPLAGPGGGGFGGDPFGAGGLGDIFQTFFGGGGGGFGGGRGPRGRVRPGEPALVGVSLDLEEIAAGARKEITVDTAVLCDTCKGAGTRDNSKPVTCATCKGAGEIQAVQRSFLGQVMTVRECPECHGVGETIPDPCLKCNGDGRVRSRRNMTVKIPAGVQDGMRVRLAGQGEVGPGGGPAGDLYVEISEQSDDVFVRDGDDLHCTVRVPMVDAALGTELTVDTVLGGSATVAVDAGTQPGTVVRVRGQGLPHLNSGVRGDLHAHLEVVVPKKVDSRQADLLKELRDVSGDEVELVTATSKAAGSGGLFSKLRNAFAGR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AEUD01000001
EMBL· GenBank· DDBJ
EGD57037.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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