F1VY41 · F1VY41_9BURK

Function

function

Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Pathway

tRNA modification; tRNA-queuosine biosynthesis.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site90Proton acceptor
Binding site90-94substrate
Binding site144substrate
Binding site193substrate
Binding site220substrate
Active site270Nucleophile
Binding site308Zn2+ (UniProtKB | ChEBI)
Binding site310Zn2+ (UniProtKB | ChEBI)
Binding site313Zn2+ (UniProtKB | ChEBI)
Binding site339Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionmetal ion binding
Molecular FunctiontRNA-guanosine(34) queuine transglycosylase activity
Biological Processqueuosine biosynthetic process
Biological ProcesstRNA wobble guanine modification
Biological ProcesstRNA-guanine transglycosylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Queuine tRNA-ribosyltransferase
  • EC number
  • Alternative names
    • Guanine insertion enzyme
    • tRNA-guanine transglycosylase

Gene names

    • Name
      tgt
    • ORF names
      IMCC9480_2441

Organism names

Accessions

  • Primary accession
    F1VY41

Proteomes

Subcellular Location

Interaction

Subunit

Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain11-371tRNA-guanine15 transglycosylase-like
Region251-257RNA binding
Region275-279RNA binding; important for wobble base 34 recognition

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    375
  • Mass (Da)
    41,744
  • Last updated
    2011-05-31 v1
  • Checksum
    E6E1619CE6F59F1E
MIDFKLLKTDGHARRGRITLNHGVIETPIFMPVGTYGSVKAMSPLELLEINAQVVLGNTFHLWLRPGIEVIAKFGGLHKFMGWDKPILTDSGGFQVFSLGAMRKISEEGVKFSSPINGDKLFLSPEISMQIQRALNSDIVMQFDECTPYEIDGRPATDAEAATSMRMSLRWAKRSHNEFVDGENPNALFGIVQGGMFEHLRDESLAGLNEIDFGGIAIGGLSVGEPKEDMMRVLAHVGPKLPANKPHYLMGVGTPEDLVAGVANGIDMFDCVMPTRNARNGWLFTRFGDIKIRNAKHKDDTRPLDETCSCYACRNFSRAYLHHLQRTGEILGARMNTVHNLHYYLQLMQDMRDAIDAGTFQSFVALFHAERARGT

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AEPR01000222
EMBL· GenBank· DDBJ
EGF32477.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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