F1SVE4 · FPOD_METMA
- ProteinF(420)H(2) dehydrogenase subunit D
- GenefpoD
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids374 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Component of the F420H2 dehydrogenase (FPO complex) which is part of the energy-conserving F420H2:heterodisulfide oxidoreductase system. The membrane-bound electron transfer system of the complex plays an important role in the metabolism of methylotrophic methanogens when the organisms grow on methanol or methylamines. Catalyzes the oxidation of methanophenazine to dihydromethanophenazine. It shuttles electrons from F420H2, via FAD and iron-sulfur (Fe-S) centers, to methanophenazine (an electron carrier in the membrane). It couples the redox reaction to proton translocation (for every two electrons transferred, two hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. It also catalyzes the oxidation of F420H2 with quinones such as 2,3-dimethyl-1,4-naphthoquinone, 2-methyl-1,4-naphthoquinone and tetramethyl-p-benzoquinone.
Catalytic activity
- methanophenazine + reduced coenzyme F420-(gamma-L-Glu)(n) = dihydromethanophenazine + H+ + oxidized coenzyme F420-(gamma-L-Glu)(n)
CHEBI:29118 + reduced coenzyme F420-(γ-L-Glu)(n) RHEA-COMP:14378 = CHEBI:50375 + CHEBI:15378 + oxidized coenzyme F420-(γ-L-Glu)(n) RHEA-COMP:12939
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
7 μM | F420H2 | 7 | 37 |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
17 μmol/min/mg | 7 | 37 |
Measured for the whole complex.
pH Dependence
Optimum pH is 8.5.
Temperature Dependence
Optimum temperature is 39 degrees Celsius.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | Methanosarcina-phenazine hydrogenase activity | |
Molecular Function | NAD binding | |
Molecular Function | nickel cation binding | |
Molecular Function | oxidoreductase activity, acting on NAD(P)H | |
Molecular Function | quinone binding | |
Molecular Function | reduced coenzyme F420 dehydrogenase activity | |
Biological Process | methanogenesis | |
Biological Process | methanol metabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameF(420)H(2) dehydrogenase subunit D
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Stenosarchaea group > Methanomicrobia > Methanosarcinales > Methanosarcinaceae > Methanosarcina
Accessions
- Primary accessionF1SVE4
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000423964 | 1-374 | F420H2 dehydrogenase subunit D | |||
Sequence: MEEMLESNEMIVHLGPQHPMQPGPFRLNLKLKGETIMDAEVEMGYIHKGIEKILENRTYLQGITIVDRICYLVALTNEECYVGCVEKLLDIEPPERAQYIRVILEELSRLQSHLLGLGEYGEFIGFVSMFMYTIKEREDILTLIDMVTGARVTHSYLRFGGVRDDLPEGFKEKTIPVLNKLKKVIRDYEEMFYSDTIYRERTIGIGVLTADEAKSLGVSGPVLRATGVPFDIRKNEPYLVYRDLDFKVCTETAGDCFARVQVRLNEMRESIYIIEQCLDMIPNGPIFPEGTPYGKRTPVMRVPAGEVFHRVEDPRGEMGMYMVSDGSDRPYRVKVRGPYYPTLQALPPLIIGTTVADMVSISGSMDGCTSEVDR |
Interaction
Subunit
The FPO complex is composed of at least 13 different subunits.
Structure
Sequence
- Sequence statusComplete
- Length374
- Mass (Da)42,492
- Last updated2011-05-03 v1
- Checksum97C19D7AF597CE99
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF228525 EMBL· GenBank· DDBJ | AAF65734.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE008384 EMBL· GenBank· DDBJ | AAM32184.1 EMBL· GenBank· DDBJ | Genomic DNA |