F1RE57 · DHC3A_DANRE
- ProteinPalmitoyltransferase ZDHHC3-A
- Genezdhhc3a
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids316 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Golgi-localized palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates and regulates their association with membranes (Probable). Has no stringent fatty acid selectivity and in addition to palmitate can also transfer onto target proteins myristate from tetradecanoyl-CoA and stearate from octadecanoyl-CoA (Probable).
Catalytic activity
- hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-hexadecanoyl-L-cysteinyl-[protein]This reaction proceeds in the forward direction.
- L-cysteinyl-[protein] + tetradecanoyl-CoA = CoA + S-tetradecanoyl-L-cysteinyl-[protein]This reaction proceeds in the forward direction.
- L-cysteinyl-[protein] + octadecanoyl-CoA = CoA + S-octadecanoyl-L-cysteinyl-[protein]This reaction proceeds in the forward direction.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 155 | S-palmitoyl cysteine intermediate | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | Golgi apparatus | |
Cellular Component | Golgi membrane | |
Molecular Function | protein homodimerization activity | |
Molecular Function | protein-cysteine S-myristoyltransferase activity | |
Molecular Function | protein-cysteine S-palmitoyltransferase activity | |
Molecular Function | protein-cysteine S-stearoyltransferase activity | |
Molecular Function | S-acyltransferase activity | |
Biological Process | peptidyl-L-cysteine S-palmitoylation | |
Biological Process | protein palmitoylation | |
Biological Process | protein targeting to membrane |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePalmitoyltransferase ZDHHC3-A
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio
Accessions
- Primary accessionF1RE57
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-45 | Cytoplasmic | ||||
Sequence: MRSPVPRFRDVERQASGLQPPQCLPSCHERQSSMWFIKDACGIVC | ||||||
Transmembrane | 46-66 | Helical | ||||
Sequence: AIITWFLVFFAEFVVLFVMLI | ||||||
Topological domain | 67-70 | Lumenal | ||||
Sequence: PSKN | ||||||
Transmembrane | 71-91 | Helical | ||||
Sequence: LTYSLVNGTLFNSLAFLALAS | ||||||
Topological domain | 92-169 | Cytoplasmic | ||||
Sequence: HFRAMCTDPGAVPKGNATKEYIESLQLKPGQVVYKCPKCCSIKPDRAHHCSVCKRCIRKMDHHCPWVNNCVGENNQKY | ||||||
Transmembrane | 170-190 | Helical | ||||
Sequence: FVLFTMYICLISLHSLVMVVF | ||||||
Topological domain | 191-212 | Lumenal | ||||
Sequence: HFLNCFEDDWTKCSTFSPPATV | ||||||
Transmembrane | 213-233 | Helical | ||||
Sequence: ILLILLCFEGLLFLIFTSVMF | ||||||
Topological domain | 234-316 | Cytoplasmic | ||||
Sequence: GTQVHSICTDETGIEKLKREDPTWEKTQCWEGMKSAFGGPLSVTWFSPFTDLSCQKDDSSPVPMFPQGEIIEEDVIEIPLEPH |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, lipidation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000449531 | 1-316 | Palmitoyltransferase ZDHHC3-A | |||
Sequence: MRSPVPRFRDVERQASGLQPPQCLPSCHERQSSMWFIKDACGIVCAIITWFLVFFAEFVVLFVMLIPSKNLTYSLVNGTLFNSLAFLALASHFRAMCTDPGAVPKGNATKEYIESLQLKPGQVVYKCPKCCSIKPDRAHHCSVCKRCIRKMDHHCPWVNNCVGENNQKYFVLFTMYICLISLHSLVMVVFHFLNCFEDDWTKCSTFSPPATVILLILLCFEGLLFLIFTSVMFGTQVHSICTDETGIEKLKREDPTWEKTQCWEGMKSAFGGPLSVTWFSPFTDLSCQKDDSSPVPMFPQGEIIEEDVIEIPLEPH | ||||||
Lipidation | 144 | S-palmitoyl cysteine | ||||
Sequence: C |
Post-translational modification
Autopalmitoylated.
Keywords
- PTM
Proteomic databases
Expression
Developmental stage
Probably maternally supplied, the zygotic expression is detected early during development at the sphere stage but decreases after 7.5 hpf.
Gene expression databases
Interaction
Subunit
Monomer. Homooligomers. The monomeric form has a higher catalytic activity. Forms heterooligomers with zdhhc7.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 124-175 | DHHC | ||||
Sequence: VYKCPKCCSIKPDRAHHCSVCKRCIRKMDHHCPWVNNCVGENNQKYFVLFTM |
Domain
The DHHC domain is required for palmitoyltransferase activity.
Sequence similarities
Belongs to the DHHC palmitoyltransferase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length316
- Mass (Da)35,892
- Last updated2011-05-03 v1
- Checksum103F2BED563EE62F
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8M2B4K7 | A0A8M2B4K7_DANRE | zdhhc3a | 297 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 32 | in Ref. 2; AAH76087 | ||||
Sequence: S → N |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CR925805 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC076087 EMBL· GenBank· DDBJ | AAH76087.1 EMBL· GenBank· DDBJ | mRNA |