F1R9Y7 · F1R9Y7_DANRE
- ProteinNADPH-dependent diflavin oxidoreductase 1
- Genendor1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids595 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of CIAPIN1, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins. It can also reduce the [2Fe-2S] cluster of CISD1 and activate this protein implicated in Fe/S cluster repair.
Catalytic activity
- 2 oxidized [2Fe-2S]-[protein] + NADPH = 2 reduced [2Fe-2S]-[protein] + NADP+ + H+
Cofactor
Protein has several cofactor binding sites:
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 12-17 | FMN (UniProtKB | ChEBI) | |||
Binding site | 59-62 | FMN (UniProtKB | ChEBI) | |||
Binding site | 97-106 | FMN (UniProtKB | ChEBI) | |||
Binding site | 132 | FMN (UniProtKB | ChEBI) | |||
Binding site | 348 | FAD (UniProtKB | ChEBI) | |||
Binding site | 380-383 | FAD (UniProtKB | ChEBI) | |||
Binding site | 414-417 | FAD (UniProtKB | ChEBI) | |||
Binding site | 458 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 513-514 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 519-523 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 594 | FAD (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | perinuclear region of cytoplasm | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | FMN binding | |
Molecular Function | NADP binding | |
Molecular Function | NADPH-hemoprotein reductase activity | |
Biological Process | iron-sulfur cluster assembly |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADPH-dependent diflavin oxidoreductase 1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio
Accessions
- Primary accessionF1R9Y7
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Concentrated in perinuclear structure.
Keywords
- Cellular component
Expression
Gene expression databases
Interaction
Subunit
Interacts with CIAPIN1; as part of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 6-150 | Flavodoxin-like | |||
Domain | 204-444 | FAD-binding FR-type | |||
Sequence similarities
Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
In the N-terminal section; belongs to the flavodoxin family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length595
- Mass (Da)66,706
- Last updated2011-05-03 v1
- ChecksumBA653DEED3E555D6
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
Q6PFP6 | NDOR1_DANRE | ndor1 | 595 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FP102160 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |