F1Q4W7 · F1Q4W7_DANRE
- ProteinHistone-lysine N-methyltransferase, H3 lysine-79 specific
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1454 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Histone methyltransferase that specifically trimethylates histone H3 to form H3K79me3. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histone.
Miscellaneous
In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.
Catalytic activity
- L-lysyl79-[histone H3] + 3 S-adenosyl-L-methionine = 3 H+ + N6,N6,N6-trimethyl-L-lysyl79-[histone H3] + 3 S-adenosyl-L-homocysteine
RHEA-COMP:15549 CHEBI:29969 Position: 79+ 3 CHEBI:59789 = 3 CHEBI:15378 + RHEA-COMP:15552 CHEBI:61961 Position: 79+ 3 CHEBI:57856
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | histone H3K79 methyltransferase activity | |
Molecular Function | histone H3K79 trimethyltransferase activity | |
Molecular Function | metal ion binding | |
Biological Process | canonical Wnt signaling pathway | |
Biological Process | DNA damage checkpoint signaling | |
Biological Process | DNA repair | |
Biological Process | intestinal epithelial structure maintenance | |
Biological Process | methylation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHistone-lysine N-methyltransferase, H3 lysine-79 specific
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio
Accessions
- Primary accessionF1Q4W7
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for signal, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-24 | |||||
Sequence: MLLFLPRITCKIVCLCVVQWKGTT | ||||||
Chain | PRO_5015355813 | 25-1454 | Histone-lysine N-methyltransferase, H3 lysine-79 specific | |||
Sequence: QPMKLNKRPSNGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQIIDEMEMMEEDTFVDLGSGVGQVVLQVAAATNCKHYYGVEKADIPATYAESMDKEFKRWMKWYGKKHGEYTLERGDFLSEEWKERIASTSVIFVNNFAFGPEVDHQLKERFANMKEGGKIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLRGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEVARRRPQKDSKENKSNTTTPTKPKEHKQAQHDSGGEEEPVVPVKPSPKPRRAKILTRGRKLGNRKRGRPKKAPAATAAERKNKKSQSALDLLHAKTLSAAPAQEPYRSPQSPFYQLPPKVQHYTSSQLLMAPTPPGLQTLLDNIKVQYLHFMAYMKTPQYRTNLQQSLEQEKLRHTELSSQAQQLVNACHAHKERIHDLFQSKLEELGVKAVTVEDLVQAQKEITAHNMQLREQTKQLEHDMAELRDQSQLLLKARCEELKLDTLCWDTLKKEIEALRRQISEKQRHCLELQISIVELEKSQRQQELLQLKSSYSPCEVSPYRKALPGPDPRPTLDPDTPKLTPQSSMGLNGLSPELSINGTASPGYERCTGSAMGKNELLTHYLPISPDHEIVPPTPDSRTRQLGQPLPDYTRFSPAKIALRRHLNQDSAANQFRALGNIHRGDTGAISSPTLGFKQASPCSSEVQAVTTAKSTDRADKEKSPTAPGDTITSLPISIPLSTVHPSKLPVSIPLASVVLPNRVERLRSTLSPVLNPAGQSNGKPPLTPTSGYSSSSGLVNGSHSTMLTEDQDCDVASPPPHSTPVMGLQPRGSGLSPPLGTGGVLQYADGPPRLPPEDGHERQGPESDTEPLDSEARRRIFFSSSSSSSSSSSSTGGSRLHHGSVKQGHHHHSKHHHHHHHHHHHHSPSSHSQEGRKRGRRKRSSSGAVPMSGSPKRRVFPGLGCSSHSSGSPLNINSMVNNINQPLEISAISSPEQSGRSPCEQDLDQPPVLKRERPLELNNTGRYSSAPSSDEEGNGYAPDTSSSSRIERKIATISLDRDGPVRDLERGLQGRKSGASSVSSEVSSSSGTSKWKSTFSPISDPKPTPPDIRQGGSPFSVGASRGGTDSDSDHKPQRRGEREREPYGNSNLFLSQDGSGRSAVNTSERSLQKQKVREWDLKSVSGLASQNLFISAAASGGILSGKVGGAATVSSASAVGQYFPLGGASVLQPLFGTQAAGPATSGAPRLVNGHSALGSFSSAGLAGGAAGGIFHHMVSSSSSQQSGPNLPSSHNSQQRHLDTNPKAGASFLASGPNRTQTVLHAPSPPTLALPPPPPLLNTVPSQPSSPRSSRPQSSIPVATSSQSLAAASSSCTSSASSRPFTVHYPPHLQPPGSAGSGGTGASWRTLASYTASQIPGNRPR | ||||||
Disulfide bond | 527 | Interchain | ||||
Sequence: C |
Proteomic databases
Expression
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, compositional bias, region, coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-261 | DOT1 | ||||
Sequence: MLLFLPRITCKIVCLCVVQWKGTTQPMKLNKRPSNGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQIIDEMEMMEEDTFVDLGSGVGQVVLQVAAATNCKHYYGVEKADIPATYAESMDKEFKRWMKWYGKKHGEYTLERGDFLSEEWKERIASTSVIFVNNFAFGPEVDHQLKERFANMKEGGKIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLRGSVSWTGKPVSYYLHTIDRTILENYFSSLK | ||||||
Compositional bias | 266-282 | Basic and acidic residues | ||||
Sequence: REEQEVARRRPQKDSKE | ||||||
Region | 266-356 | Disordered | ||||
Sequence: REEQEVARRRPQKDSKENKSNTTTPTKPKEHKQAQHDSGGEEEPVVPVKPSPKPRRAKILTRGRKLGNRKRGRPKKAPAATAAERKNKKSQ | ||||||
Compositional bias | 288-308 | Basic and acidic residues | ||||
Sequence: TTPTKPKEHKQAQHDSGGEEE | ||||||
Compositional bias | 320-341 | Basic residues | ||||
Sequence: RRAKILTRGRKLGNRKRGRPKK | ||||||
Compositional bias | 342-356 | Basic and acidic residues | ||||
Sequence: APAATAAERKNKKSQ | ||||||
Coiled coil | 487-564 | |||||
Sequence: LVQAQKEITAHNMQLREQTKQLEHDMAELRDQSQLLLKARCEELKLDTLCWDTLKKEIEALRRQISEKQRHCLELQIS | ||||||
Region | 589-621 | Disordered | ||||
Sequence: VSPYRKALPGPDPRPTLDPDTPKLTPQSSMGLN | ||||||
Region | 738-760 | Disordered | ||||
Sequence: AVTTAKSTDRADKEKSPTAPGDT | ||||||
Region | 800-1009 | Disordered | ||||
Sequence: LSPVLNPAGQSNGKPPLTPTSGYSSSSGLVNGSHSTMLTEDQDCDVASPPPHSTPVMGLQPRGSGLSPPLGTGGVLQYADGPPRLPPEDGHERQGPESDTEPLDSEARRRIFFSSSSSSSSSSSSTGGSRLHHGSVKQGHHHHSKHHHHHHHHHHHHSPSSHSQEGRKRGRRKRSSSGAVPMSGSPKRRVFPGLGCSSHSSGSPLNINSM | ||||||
Compositional bias | 802-841 | Polar residues | ||||
Sequence: PVLNPAGQSNGKPPLTPTSGYSSSSGLVNGSHSTMLTEDQ | ||||||
Compositional bias | 884-907 | Basic and acidic residues | ||||
Sequence: LPPEDGHERQGPESDTEPLDSEAR | ||||||
Compositional bias | 909-931 | Polar residues | ||||
Sequence: RIFFSSSSSSSSSSSSTGGSRLH | ||||||
Compositional bias | 933-974 | Basic residues | ||||
Sequence: GSVKQGHHHHSKHHHHHHHHHHHHSPSSHSQEGRKRGRRKRS | ||||||
Compositional bias | 995-1009 | Polar residues | ||||
Sequence: CSSHSSGSPLNINSM | ||||||
Region | 1023-1199 | Disordered | ||||
Sequence: SSPEQSGRSPCEQDLDQPPVLKRERPLELNNTGRYSSAPSSDEEGNGYAPDTSSSSRIERKIATISLDRDGPVRDLERGLQGRKSGASSVSSEVSSSSGTSKWKSTFSPISDPKPTPPDIRQGGSPFSVGASRGGTDSDSDHKPQRRGEREREPYGNSNLFLSQDGSGRSAVNTSER | ||||||
Compositional bias | 1052-1083 | Polar residues | ||||
Sequence: NNTGRYSSAPSSDEEGNGYAPDTSSSSRIERK | ||||||
Compositional bias | 1084-1103 | Basic and acidic residues | ||||
Sequence: IATISLDRDGPVRDLERGLQ | ||||||
Compositional bias | 1106-1132 | Polar residues | ||||
Sequence: KSGASSVSSEVSSSSGTSKWKSTFSPI | ||||||
Compositional bias | 1158-1176 | Basic and acidic residues | ||||
Sequence: TDSDSDHKPQRRGEREREP | ||||||
Compositional bias | 1179-1199 | Polar residues | ||||
Sequence: NSNLFLSQDGSGRSAVNTSER | ||||||
Compositional bias | 1309-1334 | Polar residues | ||||
Sequence: SSSSSQQSGPNLPSSHNSQQRHLDTN | ||||||
Region | 1309-1454 | Disordered | ||||
Sequence: SSSSSQQSGPNLPSSHNSQQRHLDTNPKAGASFLASGPNRTQTVLHAPSPPTLALPPPPPLLNTVPSQPSSPRSSRPQSSIPVATSSQSLAAASSSCTSSASSRPFTVHYPPHLQPPGSAGSGGTGASWRTLASYTASQIPGNRPR | ||||||
Compositional bias | 1358-1374 | Pro residues | ||||
Sequence: PPTLALPPPPPLLNTVP | ||||||
Compositional bias | 1375-1414 | Polar residues | ||||
Sequence: SQPSSPRSSRPQSSIPVATSSQSLAAASSSCTSSASSRPF | ||||||
Compositional bias | 1429-1454 | Polar residues | ||||
Sequence: GSGGTGASWRTLASYTASQIPGNRPR |
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,454
- Mass (Da)157,952
- Last updated2018-06-20 v2
- Checksum0ADB470976BDCDFE
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 266-282 | Basic and acidic residues | ||||
Sequence: REEQEVARRRPQKDSKE | ||||||
Compositional bias | 288-308 | Basic and acidic residues | ||||
Sequence: TTPTKPKEHKQAQHDSGGEEE | ||||||
Compositional bias | 320-341 | Basic residues | ||||
Sequence: RRAKILTRGRKLGNRKRGRPKK | ||||||
Compositional bias | 342-356 | Basic and acidic residues | ||||
Sequence: APAATAAERKNKKSQ | ||||||
Compositional bias | 802-841 | Polar residues | ||||
Sequence: PVLNPAGQSNGKPPLTPTSGYSSSSGLVNGSHSTMLTEDQ | ||||||
Compositional bias | 884-907 | Basic and acidic residues | ||||
Sequence: LPPEDGHERQGPESDTEPLDSEAR | ||||||
Compositional bias | 909-931 | Polar residues | ||||
Sequence: RIFFSSSSSSSSSSSSTGGSRLH | ||||||
Compositional bias | 933-974 | Basic residues | ||||
Sequence: GSVKQGHHHHSKHHHHHHHHHHHHSPSSHSQEGRKRGRRKRS | ||||||
Compositional bias | 995-1009 | Polar residues | ||||
Sequence: CSSHSSGSPLNINSM | ||||||
Compositional bias | 1052-1083 | Polar residues | ||||
Sequence: NNTGRYSSAPSSDEEGNGYAPDTSSSSRIERK | ||||||
Compositional bias | 1084-1103 | Basic and acidic residues | ||||
Sequence: IATISLDRDGPVRDLERGLQ | ||||||
Compositional bias | 1106-1132 | Polar residues | ||||
Sequence: KSGASSVSSEVSSSSGTSKWKSTFSPI | ||||||
Compositional bias | 1158-1176 | Basic and acidic residues | ||||
Sequence: TDSDSDHKPQRRGEREREP | ||||||
Compositional bias | 1179-1199 | Polar residues | ||||
Sequence: NSNLFLSQDGSGRSAVNTSER | ||||||
Compositional bias | 1309-1334 | Polar residues | ||||
Sequence: SSSSSQQSGPNLPSSHNSQQRHLDTN | ||||||
Compositional bias | 1358-1374 | Pro residues | ||||
Sequence: PPTLALPPPPPLLNTVP | ||||||
Compositional bias | 1375-1414 | Polar residues | ||||
Sequence: SQPSSPRSSRPQSSIPVATSSQSLAAASSSCTSSASSRPF | ||||||
Compositional bias | 1429-1454 | Polar residues | ||||
Sequence: GSGGTGASWRTLASYTASQIPGNRPR |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL773593 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BX005098 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |