F1N2W9 · MCM9_BOVIN

  • Protein
    DNA helicase MCM9
  • Gene
    MCM9
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Probably by regulating the localization of the MRN complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs. Acts as a helicase in DNA mismatch repair (MMR) following DNA replication errors to unwind the mismatch containing DNA strand. In addition, recruits MLH1, a component of the MMR complex, to chromatin. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression. Probably by regulating HR, plays a key role during gametogenesis.

Catalytic activity

Features

Showing features for binding site.

111391002003004005006007008009001,0001,100
TypeIDPosition(s)Description
Binding site351-358ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchromosome
Cellular ComponentMCM complex
Cellular ComponentMCM8-MCM9 complex
Cellular Componentnucleus
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular Functionhelicase activity
Molecular Functionsingle-stranded DNA binding
Biological ProcessDNA damage response
Biological ProcessDNA duplex unwinding
Biological Processdouble-strand break repair via homologous recombination
Biological Processfemale gamete generation
Biological Processgamete generation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA helicase MCM9
  • EC number
  • Alternative names
    • Minichromosome maintenance 9

Gene names

    • Name
      MCM9

Organism names

  • Taxonomic identifier
  • Strain
    • Hereford
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos

Accessions

  • Primary accession
    F1N2W9

Proteomes

Subcellular Location

Nucleus
Chromosome
Note: Colocalizes to nuclear foci with RPA1 following DNA damage. Localizes to double-stranded DNA breaks. Recruited to chromatin by MSH2.

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00004194751-1139DNA helicase MCM9
Modified residue761Phosphoserine
Modified residue1105Phosphoserine

Keywords

Proteomic databases

Interaction

Subunit

Component of the MCM8-MCM9 complex, which forms a hexamer composed of MCM8 and MCM9. Interacts with the DNA mismatch repair (MMR) complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1. Interacts with MLH1; the interaction recruits MLH1 to chromatin. Interacts with MSH2; the interaction recruits MCM9 to chromatin. Interacts with MSH6. Interacts with the MRN complex composed of MRE11, RAD50 and NBN/NBS1; the interaction recruits the MRN complex to DNA damage sites. Interacts with RAD51; the interaction recruits RAD51 to DNA damage sites.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain299-504MCM
Region671-693Disordered
Region707-742Disordered
Compositional bias725-739Basic and acidic residues
Region776-1022Disordered
Compositional bias779-793Basic and acidic residues
Compositional bias865-879Pro residues
Compositional bias886-903Basic and acidic residues
Compositional bias995-1011Basic and acidic residues
Region1060-1087Disordered

Sequence similarities

Belongs to the MCM family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,139
  • Mass (Da)
    126,508
  • Last updated
    2011-11-16 v2
  • Checksum
    FEFFF2D5438DDE8E
MNSDQVTLVGQVFESYVSEYHKNDILLILKERDEDAHYPVVVNAMTLFETNMEIGEYFNAFPNEVLTIFDNALRRSALTILQSLSQPEGLSMKQNLHARISGLPVCPELVREHIPKTKDVGHFLSVTGTVIRTSLVKILEFERDYMCNKCKHVFVVQADFEQYYTFFRPSSCPSLENCDSSKFTCLSDLSSPTRCRDYQEIKIQEQVQRLSVGSIPRSMQVILEDDLVDSCKSGDDITIYGVVMQRWKPFKQDVRCEVEIVLKANYIQVNNEESAGVNMDEEVRKEFEDFWEHYKSDPFAGRNEILASLCPQVFGMYLVKLAVAMVLAGGIQRTDATGTRVRGESHLLLVGDPGTGKSQFLKYAAKITPRSVLTTGIGSTSAGLTVTAVKDSGEWNLEAGALVLADAGLCCIDEFNSLKEHDRTSIHEAMEQQTISVAKAGLVCKLNTRTTILAATNPKGQYDPRESVSVNIALSSPLLSRFDLILVLLDTKNEDWDRIISSFILENKGYPSKSEKLWSMEKMKSYFCLIRKLQPTLSDEGNQVLLRYYQMQRQSDSRNAARTTIRLLESLIRLAEAHARLMFRDTVTLEDAVTVVSVMESSMQGGALLGGVNALHTSFPENPLQQYQTQCELILEKLELPNLLSEELRRLERLQNWSVDQSQPQAMEAETIPGSLGGDARKESNFRTSTQQEVNCSAHRFSIGGSFGGSPLLGPSSHLGPKGPASGKHSEEHRNSQDGSLDWFDSVATHPIEPKNTAPVSPSPKTSRGAMALKICNNRSQGKEDREAGQRSKLETEPLPAAGETETPLRPGNREGERPRKAATVSEAAVSADEPDSVLTHHVPRKLHKLHKARAQELCRNPTRPPLQPTSPSHPQPTPIQSPERVLETPKRKRQKSHAQAQEPHQESVESLGAPVAKLAKFTFKQKSKLTHSPEDRGPVSPGTSKPVVQSPENPQRRAKRGAALPGKGPEKLASRIRSSAQPQDETRGVSPQPPYKDRPEEKRERAPAKGTVQPELELGNDMGRFFLASERVRKEEVSCSNKSSKVHACTLAKLANFSFTSPSESKSESPPPPQSKNPSEGGPSCVATATALGRKRKTFQLDTSTEKLSLSKTSLFTLPELDDEPLDFDWDEEMRKKP

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias725-739Basic and acidic residues
Compositional bias779-793Basic and acidic residues
Compositional bias865-879Pro residues
Compositional bias886-903Basic and acidic residues
Compositional bias995-1011Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DAAA02025730
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

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