F1N2W9 · MCM9_BOVIN
- ProteinDNA helicase MCM9
- GeneMCM9
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids1139 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Probably by regulating the localization of the MRN complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs. Acts as a helicase in DNA mismatch repair (MMR) following DNA replication errors to unwind the mismatch containing DNA strand. In addition, recruits MLH1, a component of the MMR complex, to chromatin. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression. Probably by regulating HR, plays a key role during gametogenesis.
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chromosome | |
Cellular Component | MCM complex | |
Cellular Component | MCM8-MCM9 complex | |
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | helicase activity | |
Molecular Function | single-stranded DNA binding | |
Biological Process | DNA damage response | |
Biological Process | DNA duplex unwinding | |
Biological Process | double-strand break repair via homologous recombination | |
Biological Process | female gamete generation | |
Biological Process | gamete generation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA helicase MCM9
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos
Accessions
- Primary accessionF1N2W9
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Colocalizes to nuclear foci with RPA1 following DNA damage. Localizes to double-stranded DNA breaks. Recruited to chromatin by MSH2.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000419475 | 1-1139 | DNA helicase MCM9 | |||
Sequence: MNSDQVTLVGQVFESYVSEYHKNDILLILKERDEDAHYPVVVNAMTLFETNMEIGEYFNAFPNEVLTIFDNALRRSALTILQSLSQPEGLSMKQNLHARISGLPVCPELVREHIPKTKDVGHFLSVTGTVIRTSLVKILEFERDYMCNKCKHVFVVQADFEQYYTFFRPSSCPSLENCDSSKFTCLSDLSSPTRCRDYQEIKIQEQVQRLSVGSIPRSMQVILEDDLVDSCKSGDDITIYGVVMQRWKPFKQDVRCEVEIVLKANYIQVNNEESAGVNMDEEVRKEFEDFWEHYKSDPFAGRNEILASLCPQVFGMYLVKLAVAMVLAGGIQRTDATGTRVRGESHLLLVGDPGTGKSQFLKYAAKITPRSVLTTGIGSTSAGLTVTAVKDSGEWNLEAGALVLADAGLCCIDEFNSLKEHDRTSIHEAMEQQTISVAKAGLVCKLNTRTTILAATNPKGQYDPRESVSVNIALSSPLLSRFDLILVLLDTKNEDWDRIISSFILENKGYPSKSEKLWSMEKMKSYFCLIRKLQPTLSDEGNQVLLRYYQMQRQSDSRNAARTTIRLLESLIRLAEAHARLMFRDTVTLEDAVTVVSVMESSMQGGALLGGVNALHTSFPENPLQQYQTQCELILEKLELPNLLSEELRRLERLQNWSVDQSQPQAMEAETIPGSLGGDARKESNFRTSTQQEVNCSAHRFSIGGSFGGSPLLGPSSHLGPKGPASGKHSEEHRNSQDGSLDWFDSVATHPIEPKNTAPVSPSPKTSRGAMALKICNNRSQGKEDREAGQRSKLETEPLPAAGETETPLRPGNREGERPRKAATVSEAAVSADEPDSVLTHHVPRKLHKLHKARAQELCRNPTRPPLQPTSPSHPQPTPIQSPERVLETPKRKRQKSHAQAQEPHQESVESLGAPVAKLAKFTFKQKSKLTHSPEDRGPVSPGTSKPVVQSPENPQRRAKRGAALPGKGPEKLASRIRSSAQPQDETRGVSPQPPYKDRPEEKRERAPAKGTVQPELELGNDMGRFFLASERVRKEEVSCSNKSSKVHACTLAKLANFSFTSPSESKSESPPPPQSKNPSEGGPSCVATATALGRKRKTFQLDTSTEKLSLSKTSLFTLPELDDEPLDFDWDEEMRKKP | ||||||
Modified residue | 761 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 1105 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
Interaction
Subunit
Component of the MCM8-MCM9 complex, which forms a hexamer composed of MCM8 and MCM9. Interacts with the DNA mismatch repair (MMR) complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1. Interacts with MLH1; the interaction recruits MLH1 to chromatin. Interacts with MSH2; the interaction recruits MCM9 to chromatin. Interacts with MSH6. Interacts with the MRN complex composed of MRE11, RAD50 and NBN/NBS1; the interaction recruits the MRN complex to DNA damage sites. Interacts with RAD51; the interaction recruits RAD51 to DNA damage sites.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 299-504 | MCM | ||||
Sequence: FAGRNEILASLCPQVFGMYLVKLAVAMVLAGGIQRTDATGTRVRGESHLLLVGDPGTGKSQFLKYAAKITPRSVLTTGIGSTSAGLTVTAVKDSGEWNLEAGALVLADAGLCCIDEFNSLKEHDRTSIHEAMEQQTISVAKAGLVCKLNTRTTILAATNPKGQYDPRESVSVNIALSSPLLSRFDLILVLLDTKNEDWDRIISSFI | ||||||
Region | 671-693 | Disordered | ||||
Sequence: TIPGSLGGDARKESNFRTSTQQE | ||||||
Region | 707-742 | Disordered | ||||
Sequence: FGGSPLLGPSSHLGPKGPASGKHSEEHRNSQDGSLD | ||||||
Compositional bias | 725-739 | Basic and acidic residues | ||||
Sequence: ASGKHSEEHRNSQDG | ||||||
Region | 776-1022 | Disordered | ||||
Sequence: CNNRSQGKEDREAGQRSKLETEPLPAAGETETPLRPGNREGERPRKAATVSEAAVSADEPDSVLTHHVPRKLHKLHKARAQELCRNPTRPPLQPTSPSHPQPTPIQSPERVLETPKRKRQKSHAQAQEPHQESVESLGAPVAKLAKFTFKQKSKLTHSPEDRGPVSPGTSKPVVQSPENPQRRAKRGAALPGKGPEKLASRIRSSAQPQDETRGVSPQPPYKDRPEEKRERAPAKGTVQPELELGND | ||||||
Compositional bias | 779-793 | Basic and acidic residues | ||||
Sequence: RSQGKEDREAGQRSK | ||||||
Compositional bias | 865-879 | Pro residues | ||||
Sequence: PPLQPTSPSHPQPTP | ||||||
Compositional bias | 886-903 | Basic and acidic residues | ||||
Sequence: VLETPKRKRQKSHAQAQE | ||||||
Compositional bias | 995-1011 | Basic and acidic residues | ||||
Sequence: PYKDRPEEKRERAPAKG | ||||||
Region | 1060-1087 | Disordered | ||||
Sequence: FTSPSESKSESPPPPQSKNPSEGGPSCV |
Sequence similarities
Belongs to the MCM family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,139
- Mass (Da)126,508
- Last updated2011-11-16 v2
- ChecksumFEFFF2D5438DDE8E
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 725-739 | Basic and acidic residues | ||||
Sequence: ASGKHSEEHRNSQDG | ||||||
Compositional bias | 779-793 | Basic and acidic residues | ||||
Sequence: RSQGKEDREAGQRSK | ||||||
Compositional bias | 865-879 | Pro residues | ||||
Sequence: PPLQPTSPSHPQPTP | ||||||
Compositional bias | 886-903 | Basic and acidic residues | ||||
Sequence: VLETPKRKRQKSHAQAQE | ||||||
Compositional bias | 995-1011 | Basic and acidic residues | ||||
Sequence: PYKDRPEEKRERAPAKG |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DAAA02025730 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |