F1LP90 · MINK1_RAT

  • Protein
    Misshapen-like kinase 1
  • Gene
    Mink1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Serine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking (PubMed:21048137).
Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons (PubMed:21048137).
Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates TANC1 upon stimulation by RAP2A, MBP and SMAD1 (By similarity).
Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1 (By similarity).
Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (By similarity).

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site31-39ATP (UniProtKB | ChEBI)
Binding site54ATP (UniProtKB | ChEBI)
Active site153Proton acceptor

GO annotations

AspectTerm
Cellular Componentaxon
Cellular Componentcytoplasm
Cellular Componentdendrite
Cellular Componentglutamatergic synapse
Cellular ComponentGolgi apparatus
Cellular Componentpostsynapse
Cellular Componentpostsynaptic density
Molecular FunctionATP binding
Molecular Functionprotein serine kinase activity
Molecular Functionprotein serine/threonine kinase activity
Biological Processactin cytoskeleton organization
Biological Processbrain development
Biological Processchemical synaptic transmission
Biological Processdendrite morphogenesis
Biological ProcessMAPK cascade
Biological Processnegative thymic T cell selection
Biological Processneuron cellular homeostasis
Biological Processneuron projection morphogenesis
Biological Processpositive regulation of JNK cascade
Biological Processpositive regulation of p38MAPK cascade
Biological Processprotein autophosphorylation
Biological Processregulation of AMPA receptor activity
Biological Processregulation of cell migration
Biological Processregulation of cell-cell adhesion
Biological Processregulation of cell-matrix adhesion
Biological Processregulation of MAPK cascade
Biological Processregulation of postsynapse organization

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Misshapen-like kinase 1
  • EC number
  • Alternative names
    • GCK family kinase MiNK
    • MAPK/ERK kinase kinase kinase 6 (MEK kinase kinase 6; MEKKK 6)
    • Misshapen/NIK-related kinase
    • Mitogen-activated protein kinase kinase kinase kinase 6

Gene names

    • Name
      Mink1
    • Synonyms
      Map4k6, Mink

Organism names

  • Taxonomic identifier
  • Strain
    • Brown Norway
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    F1LP90

Proteomes

Organism-specific databases

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00004131981-1336Misshapen-like kinase 1
Modified residue324Phosphoserine
Modified residue326Phosphoserine
Modified residue502Omega-N-methylarginine
Modified residue510Omega-N-methylarginine
Modified residue643Phosphoserine
Modified residue703Phosphoserine
Modified residue756Phosphoserine
Modified residue765Phosphoserine
Modified residue781Phosphoserine
Modified residue782Phosphoserine
Modified residue786Phosphoserine
Modified residue895Phosphothreonine

Post-translational modification

Autophosphorylated.

Keywords

Proteomic databases

PTM databases

Expression

Developmental stage

Increased expression from 18 dpc to adulthood seen in cortex and hippocampus (at protein level).

Interaction

Subunit

Interacts with RAP2A and NCK1 (By similarity).
Interacts with TANC1

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, compositional bias, region.

TypeIDPosition(s)Description
Domain25-289Protein kinase
Compositional bias299-320Basic and acidic residues
Region299-347Disordered
Compositional bias321-335Acidic residues
Compositional bias363-381Polar residues
Region363-383Disordered
Compositional bias395-467Basic and acidic residues
Region395-890Disordered
Compositional bias468-499Polar residues
Compositional bias544-574Pro residues
Compositional bias634-648Polar residues
Compositional bias649-664Pro residues
Compositional bias669-689Polar residues
Compositional bias717-734Pro residues
Compositional bias769-784Polar residues
Compositional bias790-829Basic and acidic residues
Compositional bias832-848Acidic residues
Region870-1336Mediates interaction with RAP2A
Compositional bias909-937Polar residues
Region909-946Disordered
Domain1023-1310CNH

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,336
  • Mass (Da)
    150,382
  • Last updated
    2011-10-19 v2
  • Checksum
    A10289483BCD2F2D
MGDPAPARQSDFIFLVALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLNSQKQQQQQQQQQQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKTKPSSAGPEPPIPQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNTSSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPADFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEDEEEEGDGEPSEGSRDTPGGRSDGDTDSVSTMVVHDVEEVSGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSQGQSPPTKDGGGDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIVFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Computationally mapped potential isoform sequences

There are 5 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
F1LN69F1LN69_RATMink11355
A0A0G2K382A0A0G2K382_RATMink11316
A0A8I6B5X1A0A8I6B5X1_RATMink11347
A0A0G2JV77A0A0G2JV77_RATMink11346
A0A8I5ZKJ7A0A8I5ZKJ7_RATMink11332

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias299-320Basic and acidic residues
Compositional bias321-335Acidic residues
Compositional bias363-381Polar residues
Compositional bias395-467Basic and acidic residues
Compositional bias468-499Polar residues
Compositional bias544-574Pro residues
Compositional bias634-648Polar residues
Compositional bias649-664Pro residues
Compositional bias669-689Polar residues
Compositional bias717-734Pro residues
Compositional bias769-784Polar residues
Compositional bias790-829Basic and acidic residues
Compositional bias832-848Acidic residues
Compositional bias909-937Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AABR03073855
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

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