F1LM47 · F1LM47_RAT
- ProteinSuccinate--CoA ligase [ADP-forming] subunit beta, mitochondrial
- GeneSucla2
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids465 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
Catalytic activity
- succinate + ATP + CoA = succinyl-CoA + ADP + phosphate
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
Features
Showing features for site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Site | 96 | Important for substrate specificity | |||
Binding site | 100 | ATP (UniProtKB | ChEBI) | |||
Binding site | 107-109 | ATP (UniProtKB | ChEBI) | |||
Site | 164 | Important for substrate specificity | |||
Binding site | 260 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 274 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 325 | substrate; ligand shared with subunit alpha | |||
Binding site | 382-384 | substrate; ligand shared with subunit alpha | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrion | |
Cellular Component | succinate-CoA ligase complex | |
Cellular Component | succinate-CoA ligase complex (ADP-forming) | |
Molecular Function | ATP binding | |
Molecular Function | magnesium ion binding | |
Molecular Function | succinate-CoA ligase (ADP-forming) activity | |
Biological Process | succinate metabolic process | |
Biological Process | succinyl-CoA metabolic process | |
Biological Process | tricarboxylic acid cycle |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSuccinate--CoA ligase [ADP-forming] subunit beta, mitochondrial
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionF1LM47
Proteomes
Organism-specific databases
Subcellular Location
Expression
Gene expression databases
Interaction
Subunit
Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for ATP. Interacts with ALAS2.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 63-290 | ATP-grasp | |||
Sequence similarities
Belongs to the succinate/malate CoA ligase beta subunit family. ATP-specific subunit beta subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length465
- Mass (Da)50,306
- Last updated2011-05-03 v1
- Checksum4A148D18475E621D
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8I6A1U8 | A0A8I6A1U8_RAT | Sucla2 | 458 |
Keywords
- Technical term