F1CKI8 · 3CAR2_PICSI
- ProteinCarene synthase 2, chloroplastic
- GeneTPS-3car2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids627 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Terpene synthase (TPS) involved in defensive oleoresin formation in conifers in response to insect attack (e.g. white pine weevil P.strobi) or other injury.
Miscellaneous
Expressed only in resistant trees, not detected in genomic DNA of susceptible trees.
Catalytic activity
- (2E)-geranyl diphosphate = +-car-3-ene + diphosphate
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 3 Mg2+ or Mn2+ ions per subunit.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
1.47 μM | geranyl diphosphate |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
0.91 pmol/sec/ug | with geranyl diphosphate as substrate |
kcat is 0.06 sec-1 with geranyl diphosphate as substrate.
Pathway
Terpene metabolism; oleoresin biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Molecular Function | lyase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | terpene synthase activity | |
Biological Process | diterpenoid biosynthetic process | |
Biological Process | monoterpene biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCarene synthase 2, chloroplastic
- EC number
- Short namesPsTPS-3car2
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Pinopsida > Pinidae > Conifers I > Pinales > Pinaceae > Picea
Accessions
- Primary accessionF1CKI8
- Secondary accessions
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-36 | Chloroplast | ||||
Sequence: MSVISIVPLASKSCLYKSLMSSTHELKALCRPIVTL | ||||||
Chain | PRO_0000418968 | 37-627 | Carene synthase 2, chloroplastic | |||
Sequence: GMCRRGKSVMASMSTGLTTAVSDDGVQRRIGDHHSNLWDDNFIQSLSSPYRASSYGETTNKLIGEVKEIFNSLSMADGGLMSPVDDLLQHLSMVDNVERLGIDRHFQTEIKVSLDYVYSYWSEKGIGSGRDIVCTDLNTTALGFRILRLHGYTVFPDVFEHLKDQMGRIACSANHTERQISSILNLFRASLIAFPGEKVMEEAEIFSATYLKEALQTIPVSSLSQEIQYVLQYRWHSNLPRLEARTYIDILQENTKNQMLDVNTEKVLELAKLEFNIFHSLQQNELKSVSRWWKDSGFPDLNFIRHRHVEFYTLVSGIDMEPKHSTFRLSFVKMCHLITVLDDMYDTFGTIDELRLFTAAVKRWDPSTTQCLPEYMKGVYIVLYETVNEMAKEAQKSQGRDTLNYVRQALEAYIGAYHKEAEWISTGYLPTFDEYFENGKASSGHRIATLQPTFMLDIPFPHHILQEIDFPSKFNDFACSILRLRGDTRCYQADMARGEEASCISCYMKDNPGSTQEDALNHINNMIEETIKKLNRELLKPDNNVPISSKKHAFDISRGLHHFYNYRDGYTVASNETKNLVIKTVLEPVPM |
Expression
Induction
By jasmonic acid (MeJA).
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 378-382 | DDXXD motif | ||||
Sequence: DDMYD |
Sequence similarities
Belongs to the terpene synthase family. Tpsd subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length627
- Mass (Da)71,645
- Last updated2011-05-03 v1
- ChecksumE7F29AF18ED46620
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
HQ336800 EMBL· GenBank· DDBJ | ADU85926.1 EMBL· GenBank· DDBJ | mRNA | ||
HQ850276 EMBL· GenBank· DDBJ | ADY38568.1 EMBL· GenBank· DDBJ | Genomic DNA |