F0NID4 · PINA_SULIR

Function

function

Promotes Holliday junction (HJ) branch migration and unwinds Y-shaped DNA (but not replication forks or dsDNA) in an ATP hydrolysis-dependent manner (PubMed:28238763).
Stimulates cleavage by HJ resolvase Hjc (PubMed:28238763).
Unwinds Y-shaped and 3'-flap DNA substrates. In the absence of other proteins stabilizes replication forks (prevents spontaneous unwinding); Hjc, Hjm (Hel308) and PINA coordinate HJ migration and cleavage of replication forks in a coordinated way (PubMed:29846688).
Inhibits the 5'-3' (but not 3'-5') helicase activity of helicase Hjm (Hel308) on overhang DNA (PubMed:29846688).
Probably acts as an ATP-dependent pump that pulls DNA through the hexamer (By similarity).

Miscellaneous

A triple mutation (Arg-147-Lys/Ile-199-Ser/Arg-206-Ala) forms monomers rather than hexamers where the PIN and helicase domains are in very different positions than in wild-type protein, see PDB:5YWW.

Catalytic activity

Cofactor

Ca2+ (UniProtKB | Rhea| CHEBI:29108 )

Note: Ca2+ stimulates ATPase activity more than Mn2+ or Mg2+.

pH Dependence

Optimum pH is 8.0-9.0 for ATPase activity.

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular FunctionDNA binding
Molecular Functionfour-way junction helicase activity
Molecular Functionhydrolase activity
Biological ProcessDNA recombination
Biological ProcessDNA repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Holliday junction branch migration ATPase PINA
  • EC number
  • Short names
    PIN domain-containing ATPase
    ; PINA

Gene names

    • Name
      pina
    • Ordered locus names
      SiRe_1432

Organism names

Accessions

  • Primary accession
    F0NID4

Proteomes

Phenotypes & Variants

Disruption phenotype

Essential, it cannot be deleted.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis206Nearly complete loss of ATPase activity, does not unwind HJDNA.
Mutagenesis261Loss of ATPase activity, does not unwind HJDNA.
Mutagenesis322Loss of ATPase activity.
Mutagenesis325Loss of ATPase activity.
Mutagenesis493-505Nearly complete loss of interaction with Hjc and Hjm (hel308).

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004591231-505Holliday junction branch migration ATPase PINA

Interaction

Subunit

Homohexamer; the central pore (25-31 Angstroms) is large enough to hold dsDNA (PubMed:28238763).
In PDB:5F4H two of the 6 subunits are in an ATP-binding competent conformation (PubMed:28238763).
Interacts with Holliday junction resolvase Hjc; in the presence of HJ DNA this interaction decreases branch migration but not Y-DNA unwinding (PubMed:28238763).
Interacts with helicase Hjm (hel308) which decreases the DNA helicase activity of Hjm (PubMed:29846688).

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain2-106PINc
Region434-505KH domain
Region493-505Required for maximum interaction with Hjc and Hjm

Domain

The C-terminus (residues 434-505) forms a novel KH fold; this domain binds ssDNA but is not sufficient on its own to interact with Hjm (PubMed:29846688).
It is probably destabilized in the homohexamer (Probable) (PubMed:29846688).

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    505
  • Mass (Da)
    57,216
  • Last updated
    2011-05-03 v1
  • Checksum
    03F6F8F92C90F500
MNDLMLDKSALLFGVSKYLEKGIITGNVLIHKSLLAELERESNDGLVSAEIALDEVKKLKDITERILVNFEIVGDDSKKGEANELSREYCLEKGCIIVTADETQKKICDAMGIQYNFLQPLKQGLSFESFFDDETMSLHIKEDTVPRAKKGKPGNWKFVNLSDKPMLSTDVRMIANEIINAVRLIKGSFVEIERRGSLIIQLGNYRVVITRPPLSDGWEITITRPVVRKRLEDYNLDERLIKRLEERAEGIIIAGAPGMGKTTFAQALAEYYMRLGKIVKTIESPRDMHLPPEITQYSKNYAEIGELHDILLLSRPDYTVYDEMRNDEDFKLYVDLRLAGVGMVGVVHATSPIDAIHRFVNRVDIGTIPNILDTIIFINSGNVSKVYTLEMTVKVPAGLKEADLARPVVEIKDLATGNTEYEIYVFGEQTMIVPVNRGITMSNMEFKISKIVNNIIPNATVKYEDGEYVIVIPKEEIGKYNRKLVQRLKRLEKKNNIKIKIKLSD

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP002425
EMBL· GenBank· DDBJ
ADX85498.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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