F0NID4 · PINA_SULIR
- ProteinHolliday junction branch migration ATPase PINA
- Genepina
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids505 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Promotes Holliday junction (HJ) branch migration and unwinds Y-shaped DNA (but not replication forks or dsDNA) in an ATP hydrolysis-dependent manner (PubMed:28238763).
Stimulates cleavage by HJ resolvase Hjc (PubMed:28238763).
Unwinds Y-shaped and 3'-flap DNA substrates. In the absence of other proteins stabilizes replication forks (prevents spontaneous unwinding); Hjc, Hjm (Hel308) and PINA coordinate HJ migration and cleavage of replication forks in a coordinated way (PubMed:29846688).
Inhibits the 5'-3' (but not 3'-5') helicase activity of helicase Hjm (Hel308) on overhang DNA (PubMed:29846688).
Probably acts as an ATP-dependent pump that pulls DNA through the hexamer (By similarity).
Stimulates cleavage by HJ resolvase Hjc (PubMed:28238763).
Unwinds Y-shaped and 3'-flap DNA substrates. In the absence of other proteins stabilizes replication forks (prevents spontaneous unwinding); Hjc, Hjm (Hel308) and PINA coordinate HJ migration and cleavage of replication forks in a coordinated way (PubMed:29846688).
Inhibits the 5'-3' (but not 3'-5') helicase activity of helicase Hjm (Hel308) on overhang DNA (PubMed:29846688).
Probably acts as an ATP-dependent pump that pulls DNA through the hexamer (By similarity).
Miscellaneous
A triple mutation (Arg-147-Lys/Ile-199-Ser/Arg-206-Ala) forms monomers rather than hexamers where the PIN and helicase domains are in very different positions than in wild-type protein, see PDB:5YWW.
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Cofactor
Note: Ca2+ stimulates ATPase activity more than Mn2+ or Mg2+.
pH Dependence
Optimum pH is 8.0-9.0 for ATPase activity.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | DNA binding | |
Molecular Function | four-way junction helicase activity | |
Molecular Function | hydrolase activity | |
Biological Process | DNA recombination | |
Biological Process | DNA repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHolliday junction branch migration ATPase PINA
- EC number
- Short namesPIN domain-containing ATPase ; PINA
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Thermoproteota > Thermoprotei > Sulfolobales > Sulfolobaceae > Sulfolobus
Accessions
- Primary accessionF0NID4
Proteomes
Phenotypes & Variants
Disruption phenotype
Essential, it cannot be deleted.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 206 | Nearly complete loss of ATPase activity, does not unwind HJDNA. | ||||
Sequence: R → A | ||||||
Mutagenesis | 261 | Loss of ATPase activity, does not unwind HJDNA. | ||||
Sequence: K → A | ||||||
Mutagenesis | 322 | Loss of ATPase activity. | ||||
Sequence: D → A | ||||||
Mutagenesis | 325 | Loss of ATPase activity. | ||||
Sequence: R → A | ||||||
Mutagenesis | 493-505 | Nearly complete loss of interaction with Hjc and Hjm (hel308). | ||||
Sequence: Missing |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000459123 | 1-505 | Holliday junction branch migration ATPase PINA | |||
Sequence: MNDLMLDKSALLFGVSKYLEKGIITGNVLIHKSLLAELERESNDGLVSAEIALDEVKKLKDITERILVNFEIVGDDSKKGEANELSREYCLEKGCIIVTADETQKKICDAMGIQYNFLQPLKQGLSFESFFDDETMSLHIKEDTVPRAKKGKPGNWKFVNLSDKPMLSTDVRMIANEIINAVRLIKGSFVEIERRGSLIIQLGNYRVVITRPPLSDGWEITITRPVVRKRLEDYNLDERLIKRLEERAEGIIIAGAPGMGKTTFAQALAEYYMRLGKIVKTIESPRDMHLPPEITQYSKNYAEIGELHDILLLSRPDYTVYDEMRNDEDFKLYVDLRLAGVGMVGVVHATSPIDAIHRFVNRVDIGTIPNILDTIIFINSGNVSKVYTLEMTVKVPAGLKEADLARPVVEIKDLATGNTEYEIYVFGEQTMIVPVNRGITMSNMEFKISKIVNNIIPNATVKYEDGEYVIVIPKEEIGKYNRKLVQRLKRLEKKNNIKIKIKLSD |
Interaction
Subunit
Homohexamer; the central pore (25-31 Angstroms) is large enough to hold dsDNA (PubMed:28238763).
In PDB:5F4H two of the 6 subunits are in an ATP-binding competent conformation (PubMed:28238763).
Interacts with Holliday junction resolvase Hjc; in the presence of HJ DNA this interaction decreases branch migration but not Y-DNA unwinding (PubMed:28238763).
Interacts with helicase Hjm (hel308) which decreases the DNA helicase activity of Hjm (PubMed:29846688).
In PDB:5F4H two of the 6 subunits are in an ATP-binding competent conformation (PubMed:28238763).
Interacts with Holliday junction resolvase Hjc; in the presence of HJ DNA this interaction decreases branch migration but not Y-DNA unwinding (PubMed:28238763).
Interacts with helicase Hjm (hel308) which decreases the DNA helicase activity of Hjm (PubMed:29846688).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 2-106 | PINc | ||||
Sequence: NDLMLDKSALLFGVSKYLEKGIITGNVLIHKSLLAELERESNDGLVSAEIALDEVKKLKDITERILVNFEIVGDDSKKGEANELSREYCLEKGCIIVTADETQKK | ||||||
Region | 434-505 | KH domain | ||||
Sequence: PVNRGITMSNMEFKISKIVNNIIPNATVKYEDGEYVIVIPKEEIGKYNRKLVQRLKRLEKKNNIKIKIKLSD | ||||||
Region | 493-505 | Required for maximum interaction with Hjc and Hjm | ||||
Sequence: KKNNIKIKIKLSD |
Domain
The C-terminus (residues 434-505) forms a novel KH fold; this domain binds ssDNA but is not sufficient on its own to interact with Hjm (PubMed:29846688).
It is probably destabilized in the homohexamer (Probable) (PubMed:29846688).
It is probably destabilized in the homohexamer (Probable) (PubMed:29846688).
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length505
- Mass (Da)57,216
- Last updated2011-05-03 v1
- Checksum03F6F8F92C90F500
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP002425 EMBL· GenBank· DDBJ | ADX85498.1 EMBL· GenBank· DDBJ | Genomic DNA |