F0M144 · F0M144_PSEPM
- ProteinPhosphoenolpyruvate carboxykinase [GTP]
- GenepckG
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids611 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Catalytic activity
- GTP + oxaloacetate = CO2 + GDP + phosphoenolpyruvate
Cofactor
Note: Binds 1 Mn2+ ion per subunit.
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 82 | substrate | ||||
Sequence: R | ||||||
Binding site | 222-224 | substrate | ||||
Sequence: YGG | ||||||
Binding site | 231 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 251 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 273 | substrate | ||||
Sequence: S | ||||||
Binding site | 274-279 | GTP (UniProtKB | ChEBI) | ||||
Sequence: ACGKTN | ||||||
Active site | 275 | |||||
Sequence: C | ||||||
Binding site | 298 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 389-391 | substrate | ||||
Sequence: NSR | ||||||
Binding site | 391 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 422 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 517-520 | GTP (UniProtKB | ChEBI) | ||||
Sequence: FGDN |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | GTP binding | |
Molecular Function | kinase activity | |
Molecular Function | manganese ion binding | |
Molecular Function | phosphoenolpyruvate carboxykinase (GTP) activity | |
Biological Process | gluconeogenesis |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphoenolpyruvate carboxykinase [GTP]
- EC number
- Short namesPEP carboxykinase ; PEPCK
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Micrococcales > Micrococcaceae > Pseudarthrobacter
Accessions
- Primary accessionF0M144
Proteomes
Subcellular Location
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 23-243 | Phosphoenolpyruvate carboxykinase GTP-utilising N-terminal | ||||
Sequence: WVEEVAELTQPDRIYWVDGSEEENTRLTDELVAAGTLTRLNQDLFPNSFAAFSDPADVARVEEQTFICSENKRDAGFTNNWMAPAEMKDKLRGLFAGSMRGRTMYVIPFVMGHLDAEDPKFGVEITDSAYVVASMRIMANIGTAVLDKITETNAFFVPALHSLGAPLEPGQADVPWPCNPDKWIVHFPEERSIWSFGSGYGGNALLGKKCYALRIASVMAR | ||||||
Domain | 247-606 | Phosphoenolpyruvate carboxykinase C-terminal P-loop | ||||
Sequence: WLAEHMLILKLTSPEKKNYYISAAFPSACGKTNLALLDPTIEGWEVETLGDDITWMRIGKEGELRAINPEAGLFGVAPGTGWGTNPNAMRAIAKGHSIFTNVALTDDGGVWWEGMTDEVPAHLTDWQGNSWTPDSDKPAAHPNSRFCTPISQIDMLAEEYYSPEGVELSAILFGGRRKTTVPLVTQARSWTNGIFMGSTLSSETTAAAAGQVGVLRRDPMAMLPFIGYDAGDYLKHWISVSGKANPERLPHIFLVNWFRRTAEGGFAWPGFGDNARVLKWAIERLEGKAEAVETPIGYVPAGDSLDLTGLDLTHAHVEEAVRVDREEWDAELASIEDWYAQFGDSLPDALRAELEALKER |
Sequence similarities
Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length611
- Mass (Da)67,185
- Last updated2011-05-03 v1
- Checksum3A3D8B00B7E3D1DD
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP002379 EMBL· GenBank· DDBJ | ADX71927.1 EMBL· GenBank· DDBJ | Genomic DNA |