E9PTA2 · TRPM2_RAT

  • Protein
    Transient receptor potential cation channel subfamily M member 2
  • Gene
    Trpm2
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Nonselective, voltage-independent cation channel that mediates Na+ and Ca2+ influx, leading to increased cytoplasmic Ca2+ levels (PubMed:11804595, PubMed:16260005, PubMed:16601673, PubMed:16651700, PubMed:19454650).
Functions as a ligand-gated ion channel. Binding of ADP-ribose to the cytoplasmic Nudix domain causes a conformation change; the channel is primed but still requires Ca2+ binding to trigger channel opening. Extracellular calcium passes through the channel and increases channel activity (By similarity).
Also contributes to Ca2+ release from intracellular stores in response to ADP-ribose (PubMed:19454650).
Plays a role in numerous processes that involve signaling via intracellular Ca2+ levels (Probable). Besides, mediates the release of lysosomal Zn2+ stores in response to reactive oxygen species, leading to increased cytosolic Zn2+ levels (PubMed:25562606).
Activated by moderate heat (35 to 40 degrees Celsius) (PubMed:16601673).
Activated by intracellular ADP-ribose, beta-NAD (NAD+) and similar compounds, and by oxidative stress caused by reactive oxygen or nitrogen species (PubMed:16260005, PubMed:16601673, PubMed:25562606).
The precise physiological activators are under debate; the true, physiological activators may be ADP-ribose and ADP-ribose-2'-phosphate. Activation by ADP-ribose and beta-NAD is strongly increased by moderate heat (35 to 40 degrees Celsius) (By similarity).
Likewise, reactive oxygen species lower the threshold for activation by moderate heat (37 degrees Celsius). Plays a role in mediating behavorial and physiological responses to moderate heat and thereby contributes to body temperature homeostasis. Plays a role in insulin secretion, a process that requires increased cytoplasmic Ca2+ levels (PubMed:16601673).
Required for normal IFNG and cytokine secretion and normal innate immune immunity in response to bacterial infection. Required for normal phagocytosis and cytokine release by macrophages exposed to zymosan (in vitro). Plays a role in dendritic cell differentiation and maturation, and in dendritic cell chemotaxis via its role in regulating cytoplasmic Ca2+ levels (By similarity).
Plays a role in the regulation of the reorganization of the actin cytoskeleton and filopodia formation in response to reactive oxygen species via its function in increasing cytoplasmic Ca2+ and Zn2+ levels (By similarity).
Confers susceptibility to cell death following oxidative stress (PubMed:11804595, PubMed:16651700, PubMed:19454650, PubMed:25562606).

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site841Ca2+ (UniProtKB | ChEBI)
Binding site844Ca2+ (UniProtKB | ChEBI)
Binding site867Ca2+ (UniProtKB | ChEBI)
Binding site1071Ca2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcell projection
Cellular Componentcytoplasmic vesicle membrane
Cellular Componentlysosomal membrane
Cellular Componentlysosome
Cellular Componentneuronal cell body
Cellular Componentperikaryon
Cellular Componentplasma membrane
Molecular Functioncalcium ion binding
Molecular Functionintracellularly gated calcium channel activity
Molecular Functionligand-gated calcium channel activity
Molecular Functionmanganese ion transmembrane transporter activity
Molecular Functionmono-ADP-D-ribose binding
Molecular Functionmonoatomic cation channel activity
Molecular Functionsodium channel activity
Biological Processcalcium ion import across plasma membrane
Biological Processcalcium ion transmembrane import into cytosol
Biological Processcalcium ion transmembrane transport
Biological Processcellular response to calcium ion
Biological Processcellular response to hydrogen peroxide
Biological Processcellular response to purine-containing compound
Biological Processcellular response to temperature stimulus
Biological Processdendritic cell chemotaxis
Biological Processdendritic cell differentiation
Biological Processestrous cycle
Biological Processmanganese ion transport
Biological Processpositive regulation of cellular response to oxidative stress
Biological Processpositive regulation of insulin secretion
Biological Processprotein homotetramerization
Biological Processregulation of actin cytoskeleton organization
Biological Processregulation of filopodium assembly
Biological Processrelease of sequestered calcium ion into cytosol
Biological Processresponse to heat
Biological Processresponse to hydroperoxide
Biological Processresponse to purine-containing compound
Biological Processtemperature homeostasis
Biological Processzinc ion transmembrane transport

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Transient receptor potential cation channel subfamily M member 2

Gene names

    • Name
      Trpm2

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Brown Norway
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    E9PTA2
  • Secondary accessions
    • Q2PH54
    • Q5G856

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
; Multi-pass membrane protein
Perikaryon
Cell projection
Cytoplasmic vesicle
Lysosome
Note: Detected at the cell membrane and in intracellular vesicles in cortical neurons. Detected on neuronal cell bodies and neurites (PubMed:16651700).
Detected on the cell membrane in polymorphonuclear neutrophils. Detected on cytoplasmic vesicles and lysosomes in immature bone marrow dendritic cells (By similarity).

Features

Showing features for topological domain, intramembrane, transmembrane.

TypeIDPosition(s)Description
Topological domain1-751Cytoplasmic
Intramembrane752-768
Topological domain769-793Cytoplasmic
Transmembrane794-814Helical
Topological domain815-825Extracellular
Transmembrane826-846Helical
Topological domain847-865Cytoplasmic
Transmembrane866-886Helical
Topological domain887-894Extracellular
Transmembrane895-915Helical
Topological domain916-927Cytoplasmic
Transmembrane928-948Helical
Topological domain949-968Extracellular
Intramembrane969-983Pore-forming
Topological domain984-1020Extracellular
Transmembrane1021-1042Helical
Topological domain1043-1077Cytoplasmic
Intramembrane1078-1096
Topological domain1097-1507Cytoplasmic

Keywords

Phenotypes & Variants

PTM/Processing

Features

Showing features for chain, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00004381921-1507Transient receptor potential cation channel subfamily M member 2
Disulfide bond994↔1006

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Detected in pancreas beta-cells (PubMed:16601673).
Detected in fetal brain cortex neurons (at protein level) (PubMed:16651700).

Gene expression databases

Interaction

Subunit

Homotetramer.

Protein-protein interaction databases

Chemistry

Structure

Family & Domains

Features

Showing features for compositional bias, region, domain, motif.

TypeIDPosition(s)Description
Compositional bias1-19Basic and acidic residues
Region1-24Disordered
Domain1351-1502Nudix hydrolase
Motif1387-1408Nudix box

Domain

The cytosolic nudix box binds ADP-ribose and is required for channel activation by ADP-ribose.

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,507
  • Mass (Da)
    172,259
  • Last updated
    2015-07-22 v3
  • Checksum
    3E54E154A0166F99
MEPLDQRRTDSDQEEGFGVQSRRATDLGMVPNLRRSNSSLCKSRRLLCSFSSEKQENLSSWIPENIKKKECVYFVESSKLSDAGKVVCECGYTHEQHIEVAIKPHTFQGKEWDPKKHVHEMPTDAFGDIVFTGLSQKVGKYVRLSQDTSSIVIYQLMTQHWGLDVPSLLISVTGGAKNFNMKLRLKSIFRRGLVKVAQTTGAWIITGGSHTGVMKQVGEAVRDFSLSSSCKEGDVITIGIATWGTIHNREALIHPMGGFPAEYMLDEEGQGNLTCLDSNHSHFILVDDGTHGQYGVEIPLRTKLEKFISEQTKERGGVAIKIPIVCVVLEGGPGTLHTIYNAITNGTPCVIVEGSGRVADVIAQVAALPVSEITISLIQQKLSVFFQEMFETFTENQIVEWTKKIQDIVRRRQLLTVFREGKDGQQDVDVAILQALLKASRSQDHFGHENWDHQLKLAVAWNRVDIARSEIFTDEWQWKPSDLHPMMTAALISNKPEFVRLFLENGVRLKEFVTWDTLLCLYENLEPSCLFHSKLQKVLAEEHERLAYASETPRLQMHHVAQVLRELLGDSTQLLYPRPRYTDRPRLSLPMPHIKLNVQGVSLRSLYKRSTGHVTFTIDPVRDLLIWAIIQNHRELAGIIWAQSQDCTAAALACSKILKELSKEEEDTDSSEEMLALADEFEHRAIGVFTECYRKDEERAQKLLVRVSEAWGKTTCLQLALEAKDMKFVSHGGIQAFLTKVWWGQLCVDNGLWRIILCMLAFPLLFTGFISFREKRLQALCRLARVRAFFNAPVVIFYLNILSYFAFLCLFAYVLMVDFQPSPSWCEYLIYLWLFSLVCEETRQLFYDPDGCGLMKMASLYFSDFWNKLDVGAILLFIAGLTCRLIPATLYPGRIILSLDFIMFCLRLMHIFTISKTLGPKIIIVKRMMKDVFFFLFLLAVWVVSFGVAKQAILIHNESRVDWIFRGVIYHSYLTIFGQIPTYIDGVNFSMDQCSPNGTDPYKPKCPESDWTGQAPAFPEWLTVTLLCLYLLFANILLLNLLIAMFNYTFQEVQEHTDQIWKFQRHDLIEEYHGRPPAPPPLILLSHLQLLIKRIVLKIPAKRHKQLKNKLEKNEEAALLSWELYLKENYLQNQQYQHKQRPEQKIQDISEKVDTMVDLLDMDRVKRSGSTEQRLASLEEQVTQMGRSLHWIVTTLKDSGFGSGAGALTLAQRAFDEPDAELSIRKKGEEGGDGYHVSARHLLYPDARIMRFPVPNEKVPWEAEFLIYDPPFYTAEKKDATLTDPVGDTAEPLSKINYNVVDGLMDRCSFHGTYVVQYGFPLNPMGRTGLRGRGSLSWFGPNHTLQPVVTRWKRNQGGGICRKSVRKMLEVLVVKLPQSEHWALPGGSREPGKMLPRKLKQVLQQEYWVTFETLLRQGTEVYKGYVDDPRNTDNAWIETVAVSIHFQDQNDVELKRLEENLQTHDPKESARGLEMSTEWQVVDRRIPLYVNHKKILQKVASLFGAHF

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8L2UH90A0A8L2UH90_RATTrpm21508

Features

Showing features for compositional bias, sequence conflict.

TypeIDPosition(s)Description
Compositional bias1-19Basic and acidic residues
Sequence conflict783in Ref. 2; AAW65801 and 1; BAE72117
Sequence conflict1210in Ref. 2; AAW65801
Sequence conflict1374in Ref. 2; AAW65801 and 1; BAE72117

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB193179
EMBL· GenBank· DDBJ
BAE72117.1
EMBL· GenBank· DDBJ
mRNA
AY749166
EMBL· GenBank· DDBJ
AAW65801.1
EMBL· GenBank· DDBJ
mRNA
AABR07044580
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AABR07044581
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AABR07072820
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AC109383
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

Disclaimer

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