E9PL17 · E9PL17_HUMAN

  • Protein
    Polyribonucleotide 5'-hydroxyl-kinase Clp1
  • Gene
    CLP1
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Ni2+ (UniProtKB | Rhea| CHEBI:49786 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site33ATP (UniProtKB | ChEBI)
Binding site73ATP (UniProtKB | ChEBI)
Binding site135-140ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular ComponentmRNA cleavage factor complex
Cellular Componentnucleoplasm
Cellular ComponenttRNA-intron endonuclease complex
Molecular FunctionATP binding
Molecular FunctionATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity
Molecular FunctionATP-dependent polyribonucleotide 5'-hydroxyl-kinase activity
Biological ProcessmRNA 3'-end processing
Biological ProcessRISC complex assembly
Biological ProcesstRNA splicing, via endonucleolytic cleavage and ligation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Polyribonucleotide 5'-hydroxyl-kinase Clp1
  • EC number
  • Alternative names
    • Polyadenylation factor Clp1
    • Polynucleotide kinase Clp1
    • Pre-mRNA cleavage complex II protein Clp1

Gene names

    • Name
      CLP1

Organism names

  • Taxonomic identifier
  • Organism
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo

Accessions

  • Primary accession
    E9PL17

Proteomes

Organism-specific databases

Subcellular Location

Keywords

  • Cellular component

Disease & Variants

Variants

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The viewer provides 433 variants from UniProt as well as other sources including ClinVar and dbSNP.

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Genetic variation databases

PTM/Processing

Features

Showing features for modified residue (large scale data).

TypeIDPosition(s)SourceDescription
Modified residue (large scale data)355PRIDEPhosphoserine
Modified residue (large scale data)367PRIDEPhosphothreonine

Proteomic databases

Expression

Gene expression databases

Interaction

Subunit

Component of the tRNA splicing endonuclease complex, composed of CLP1, TSEN2, TSEN15, TSEN34 and TSEN54. Component of pre-mRNA cleavage complex II (CF-II). Also associates with numerous components of the pre-mRNA cleavage complex I (CF-I/CFIm), including NUDT21, CPSF2, CPSF3, CPSF6 and CPSF7. Interacts with CSTF2 and SYMPK.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region1-24Disordered
Compositional bias8-24Basic and acidic residues
Domain27-118Clp1 N-terminal beta-sandwich
Domain132-318Polyribonucleotide 5'-hydroxyl-kinase Clp1 P-loop
Domain324-434Pre-mRNA cleavage complex subunit Clp1 C-terminal

Sequence similarities

Belongs to the Clp1 family. Clp1 subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    436
  • Mass (Da)
    48,940
  • Last updated
    2011-04-05 v1
  • Checksum
    51576E4951199285
MTDLHSSYTEEMGEEANDDKKPTTKFELERETELRFEVEASQSVQLELLTGMAEIFGTELTRNKKFTFDAGAKVAVFTWHGCSVQLSGRTEVAYVSKDTPMLLYLNTHTALEQMRRQAEKEEERGPRVMVVGPTDVGKSTVCRLLLNYAVRLGRRPTYVELDVGQGSVSIPGTMGALYIERPADVEEGFSIQAPLVYHFGSTTPGTNIKLYNKITSRLADVFNQRCEVNRRASVSGCVINTCGWVKGSGYQALVHAASAFEVDVVVVLDQERLYNELKRDLPHFVRTVLLPKSGGVVERSKDFRRECRDERIREYFYGFRGCFYPHAFNVKFSDVKIYKVGAPTIPDSCLPLGMSQEDNQLKLVPVTPGRDMVHHLLSVSTAEGTEENLSETSVAGFIVVTSVDLEHQVFTVLSPAPRPLPKNFLLIMDIRFMDLK

Computationally mapped potential isoform sequences

There are 3 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
Q92989CLP1_HUMANCLP1425
E9PKV5E9PKV5_HUMANCLP1210
E9PJM4E9PJM4_HUMANCLP1132

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias8-24Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP000662
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
KF511152
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

Disclaimer

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