E9NX73 · 2SS_FAGTA

Function

function

Seed storage protein.

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Molecular FunctionIgE binding
Molecular Functionnutrient reservoir activity

Keywords

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    2S seed storage albumin protein
  • Alternative names
    • 16 kDa allergen Fag t 2
    • 16 kDa major allergen
    • 2S albumin
    • 2S seed storage protein
    • Major allergen Fag t 2
  • Allergen name
    Fag t 2.0101

Organism names

Accessions

  • Primary accession
    E9NX73

Phenotypes & Variants

Allergenic properties

Causes an allergic reaction in human. Binds to IgE of patients allergic to buckwheat (PubMed:21645012, PubMed:29306152, PubMed:33412487).
Binds to IgE in 74% of the 23 tartarian buckwheat-allergic patients tested (PubMed:29306152).
IgE-binding of the natural and recombinant protein is unaffected by heat treatment or pepsin digestion (PubMed:21645012, PubMed:29306152).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis38No effect in a competitive IgE inhibition ELISA.
Mutagenesis5221% inhibitory efficiency in a competitive IgE inhibition ELISA.
Mutagenesis71No effect in a competitive IgE inhibition ELISA.
Mutagenesis87-8833% inhibitory efficiency in a competitive IgE inhibition ELISA.
Mutagenesis10043% inhibitory efficiency in a competitive IgE inhibition ELISA.
Mutagenesis13255% inhibitory efficiency in a competitive IgE inhibition ELISA.

Keywords

Protein family/group databases

PTM/Processing

Features

Showing features for signal, chain, disulfide bond.

TypeIDPosition(s)Description
Signal1-22
ChainPRO_500324388823-1492S seed storage albumin protein
Disulfide bond38↔98
Disulfide bond52↔87
Disulfide bond88↔133
Disulfide bond100↔140

Keywords

Expression

Tissue specificity

Expressed in seeds (at protein level) (PubMed:21645012, PubMed:29306152).
Expressed in seeds (PubMed:29306152).

Developmental stage

Expressed in developing seeds. Expressed in embryo, but not in endosperm (at protein level).

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region41-53No IgE-binding
Region68-81No IgE-binding
Region84-95No IgE-binding
Region97-105No IgE-binding
Region108-117IgE-binding
Region121-131No IgE-binding
Region132-141IgE-binding

Sequence similarities

Belongs to the 2S seed storage albumins family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    149
  • Mass (Da)
    17,280
  • Last updated
    2011-04-05 v1
  • Checksum
    AAC1B76E740AA260
MKLFIILATATLLIAATQAKYLRDEGFDLGETQMSSKCTRQVKMMEPELVKCNRYIAMDIMDDKYEEALSRIQGEGCESEEKFLRGCCVAMKEMEDECVCEWMKMMVENQKGRIGETLMRKGIRDLKELPNKCGISEMECHSRGNWYYV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
HQ829975
EMBL· GenBank· DDBJ
ADW27428.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

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