E9JFX9 · DMOA_HYPSL
- ProteinDimethyl-sulfide monooxygenase
- GenedmoA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids480 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Monooxygenase that mediates oxidation of dimethyl sulfide, the first step in dimethyl sulfide degradation pathway. Has much lower activity with diethyl sulfide and other short-chain alkyl methyl sulfides.
Miscellaneous
DmoB has not been identified yet. Only a peptide from the protein encoded by a putative flavin reductase (AC E0XCR3) was obtained by mass spectrometry, which is insufficient for linking this gene to DmoB (PubMed:21216999).
Catalytic activity
- dimethyl sulfide + H+ + NADH + O2 = formaldehyde + H2O + methanethiol + NAD+
Cofactor
Activity regulation
Inhibited by umbelliferone, 8-anilinonaphthalenesulfonate, a range of metal-chelating agents, and Hg2+, Cd2+ and Pb2+ ions.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
17.2 μM | Dimethyl sulfide |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
1.25 μmol/min/mg |
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | dimethyl sulfide monooxygenase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDimethyl-sulfide monooxygenase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Hyphomicrobiaceae > Hyphomicrobium
Accessions
- Primary accessionE9JFX9
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000418929 | 1-480 | Dimethyl-sulfide monooxygenase | |||
Sequence: MKKRIVLNAFDMTCVSHQSAGTWRHPSSQAARYNDLEYWTNMAMELERGCFDCLFIADVVGVYDVYRGSAEMALRDADQVPVNDPFGAISAMAAVTEHVGFGVTAAITFEQPYLLARRLSTLDHLTKGRVAWNVVSSYLNSAALNIGMDQQLAHDERYEMADEYMEVMYKLWEGSWEDDAVKRDKKSGVFTDGSKVHPINHQGKYYKVPGFHICEPSPQRTPVIFQAGASGRGSKFAASNAEGMFILTTSVEQARQITTDIRNQAEAAGRSRDSIKIFMLLTVITGDSDEAAEAKYQEYLSYANPEGMLALYGGWTGIDFAKLDPDEPLQAMENDSLRTTLESLTHGENAKKWTVRDVIRERCIGGLGPVLVGGPQKVADELERWVDEGGVDGFNLAYAVTPGSVTDFIDYIVPELRKRGRAQDSYKPGSLRRKLIGTNDGRVESTHPAAQYRDAYVGKESVADRTQPSPFANAKAPVAE |
Interaction
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 423-480 | Disordered | ||||
Sequence: QDSYKPGSLRRKLIGTNDGRVESTHPAAQYRDAYVGKESVADRTQPSPFANAKAPVAE |
Sequence similarities
Belongs to the NtaA/SnaA/DszA monooxygenase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length480
- Mass (Da)53,116
- Last updated2011-04-05 v1
- Checksum0AEC2DE5548A33FF
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
GQ980036 EMBL· GenBank· DDBJ | ADU77278.1 EMBL· GenBank· DDBJ | Genomic DNA |