E9GMP1 · E9GMP1_DAPPU

Function

function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N6-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site119[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site124[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site130[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site150[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site154[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site157[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site365[4Fe-4S] cluster 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentmitochondrion
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionlipoate synthase activity
Molecular Functionmetal ion binding
Biological Processlipoate biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Lipoyl synthase, mitochondrial
  • EC number
  • Alternative names
    • Lipoate synthase
      (LS
      ; Lip-syn
      )
    • Lipoic acid synthase

Gene names

    • ORF names
      DAPPUDRAFT_304936

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Crustacea > Branchiopoda > Diplostraca > Cladocera > Anomopoda > Daphniidae > Daphnia

Accessions

  • Primary accession
    E9GMP1

Proteomes

Subcellular Location

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain133-354Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    385
  • Mass (Da)
    42,905
  • Last updated
    2011-04-05 v1
  • Checksum
    F1C2CC657A667CCD
MAIGHGHYKIFCGRTLFNSLGSRNLVALQYSSTVAGTNNAKSSTSKIKERLENGPDFSDFVQGKVSSDENWKDYAGKLVREKGDKRLRLPPWLKTEIPVGKNFAKLKENLRGLNLHTVCEEARCPNIGECWGGGPDATATATIMLMGDTCTRGCRFCSVKTSKAPPPLDPNEPVNTAIAIAAWGLDYVVLTSVDRDDLPDQGSNHLSQTVSELKQRKPELLVECLTPDFRGDRDCVERIVLSGLDVFAHNIETVEELTWLVRDPRAKYQQSMDVLRYAKQVRPDVVTKSSIMLGLGETDDQVRKTLEDLRAANVDCVTLGQYMQPTKRHLKVTEYVTPEKFAQWESVGKELGFLYTASGPLVRSSYKAGEFFIKNIIQNAKKSRV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
GL732553
EMBL· GenBank· DDBJ
EFX79130.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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