E9EWG7 · E9EWG7_METRA

Function

function

Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

biotin (UniProtKB | Rhea| CHEBI:57586 )

Pathway

Carbohydrate biosynthesis; gluconeogenesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site148ATP (UniProtKB | ChEBI)
Binding site232ATP (UniProtKB | ChEBI)
Binding site267ATP (UniProtKB | ChEBI)
Active site324
Binding site577Mn2+ (UniProtKB | ChEBI)
Binding site649substrate
Binding site745Mn2+ (UniProtKB | ChEBI); via carbamate group
Binding site775Mn2+ (UniProtKB | ChEBI)
Binding site777Mn2+ (UniProtKB | ChEBI)
Binding site910substrate

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular Functionpyruvate carboxylase activity
Biological Processgluconeogenesis
Biological Processpyruvate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pyruvate carboxylase
  • EC number

Gene names

    • ORF names
      MAA_04366

Organism names

Accessions

  • Primary accession
    E9EWG7

Proteomes

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue745N6-carboxylysine
Modified residue1147N6-biotinyllysine

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain39-482Biotin carboxylation
Domain152-349ATP-grasp
Domain568-836Pyruvate carboxyltransferase
Domain1106-1181Lipoyl-binding

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,183
  • Mass (Da)
    129,555
  • Last updated
    2015-03-04 v2
  • Checksum
    67C6C9BA3890443E
MAVPQQPLTFNDVFEDEDVDEPKAANSVHHIRANSSIMQLKKILVANRGEIPIRIFRTAHELSLHTIAVFRQKADEAYVIGKRGQYTPVGAYLAGDEIIKIAVEHGAQLIHPGYGFLSENAEFARNVEKAGLIFVGPQPDVIDALGDKVSARKLAIAADVPVVPGTEGAVAKYEEVKAFTDTYGFPIIIKAAYGGGGRGMRVVRDAESLKESFERATSEAKSAFGNGTVFVERFLDKPKHIEVQLLGDNHGNIVHLYERDCSVQRRHQKVVEIAPAKDLPAETRDAILADAVKLAKSVNYRNAGTAEFLVDQQNRYYFIEINPRIQVEHTITEEITGIDIVAAQIQIAAGATLSQLGLTQDRISTRGFAIQCRITTEDPAEQFRPDTGKIEVYRSAGGNGVRLDGGNGFAGAVITPYYDSMLVKCTCHGSTYEIARRKVLRALIEFRVRGVKTNIPFLASLLTHPVFIDGNCWTTFIDDTPELFDLVGSQNRAQKLLAYLGDVAVNGSSIKGQIGEPKLKTEIILPELIASDGQKIDVSQPCQKGWRNILVEQGPKAFAKAVREYKGCLLMDTTWRDAHQSLLATRVRTVDLLNIAKETSHAMSNLYSLECWGGATFDVAMRFLYEDPWDRLRKMRKLVPNIPFQMLLRGANGVAYSSLPDNAIDHFVEQAKKNGVDIFRVFDALNDIDQLEVGIKAVQKAGGVAEGTVCISGDMLNPKKKYNLEYYLDLVDKLVKLDIDVLGIKDMAGVLKPHAATLLIGSIRKKYPDLPIHVHTHDSAGTGVASMVACAKAGADAVDAATDSLSGMTSQPSINAILASLEGTGLEPGLDARQVRALDTYWSQLRLLYSPFEAHLAGPDPEVYEHEIPGGQLTNMMFQASQLGLGSQWLETKKAYEHANDLLGDIVKVTPTSKVVGDLAQFMVSNKLSAEDVKARASELDFPGSVLEFLEGLMGQPYGGFPEPLRSDALRGRRKLDKRPGLFLEPVDFAKVKKDLAKKYGAPVTECDIASYVMYPKVFEDYKKFQLQYGDLSVLPTRYFLTKPEIGEEFNVELEKGKVLILKLLAVGPLSENTGQREVFFEMNGEVRQVAVLDNKAAVENISRPKADASDSSQVGAPMSGVLVELRVHEGSDVKKGDPLAVLSAMKMEMVISAPHSGKVASLQVKEGDSVDGSDLVCRITKA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
ADNJ02000005
EMBL· GenBank· DDBJ
EFZ00589.2
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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