E9EWG7 · E9EWG7_METRA
- ProteinPyruvate carboxylase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1183 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Catalytic activity
- ATP + hydrogencarbonate + pyruvate = ADP + H+ + oxaloacetate + phosphate
Cofactor
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 148 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 232 | ATP (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 267 | ATP (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Active site | 324 | |||||
Sequence: R | ||||||
Binding site | 577 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 649 | substrate | ||||
Sequence: R | ||||||
Binding site | 745 | Mn2+ (UniProtKB | ChEBI); via carbamate group | ||||
Sequence: K | ||||||
Binding site | 775 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 777 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 910 | substrate | ||||
Sequence: T |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | pyruvate carboxylase activity | |
Biological Process | gluconeogenesis | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate carboxylase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Hypocreales > Clavicipitaceae > Metarhizium
Accessions
- Primary accessionE9EWG7
Proteomes
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 745 | N6-carboxylysine | ||||
Sequence: K | ||||||
Modified residue | 1147 | N6-biotinyllysine | ||||
Sequence: K |
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 39-482 | Biotin carboxylation | ||||
Sequence: QLKKILVANRGEIPIRIFRTAHELSLHTIAVFRQKADEAYVIGKRGQYTPVGAYLAGDEIIKIAVEHGAQLIHPGYGFLSENAEFARNVEKAGLIFVGPQPDVIDALGDKVSARKLAIAADVPVVPGTEGAVAKYEEVKAFTDTYGFPIIIKAAYGGGGRGMRVVRDAESLKESFERATSEAKSAFGNGTVFVERFLDKPKHIEVQLLGDNHGNIVHLYERDCSVQRRHQKVVEIAPAKDLPAETRDAILADAVKLAKSVNYRNAGTAEFLVDQQNRYYFIEINPRIQVEHTITEEITGIDIVAAQIQIAAGATLSQLGLTQDRISTRGFAIQCRITTEDPAEQFRPDTGKIEVYRSAGGNGVRLDGGNGFAGAVITPYYDSMLVKCTCHGSTYEIARRKVLRALIEFRVRGVKTNIPFLASLLTHPVFIDGNCWTTFIDDTPE | ||||||
Domain | 152-349 | ATP-grasp | ||||
Sequence: RKLAIAADVPVVPGTEGAVAKYEEVKAFTDTYGFPIIIKAAYGGGGRGMRVVRDAESLKESFERATSEAKSAFGNGTVFVERFLDKPKHIEVQLLGDNHGNIVHLYERDCSVQRRHQKVVEIAPAKDLPAETRDAILADAVKLAKSVNYRNAGTAEFLVDQQNRYYFIEINPRIQVEHTITEEITGIDIVAAQIQIAA | ||||||
Domain | 568-836 | Pyruvate carboxyltransferase | ||||
Sequence: CLLMDTTWRDAHQSLLATRVRTVDLLNIAKETSHAMSNLYSLECWGGATFDVAMRFLYEDPWDRLRKMRKLVPNIPFQMLLRGANGVAYSSLPDNAIDHFVEQAKKNGVDIFRVFDALNDIDQLEVGIKAVQKAGGVAEGTVCISGDMLNPKKKYNLEYYLDLVDKLVKLDIDVLGIKDMAGVLKPHAATLLIGSIRKKYPDLPIHVHTHDSAGTGVASMVACAKAGADAVDAATDSLSGMTSQPSINAILASLEGTGLEPGLDARQVR | ||||||
Domain | 1106-1181 | Lipoyl-binding | ||||
Sequence: KADASDSSQVGAPMSGVLVELRVHEGSDVKKGDPLAVLSAMKMEMVISAPHSGKVASLQVKEGDSVDGSDLVCRIT |
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,183
- Mass (Da)129,555
- Last updated2015-03-04 v2
- Checksum67C6C9BA3890443E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
ADNJ02000005 EMBL· GenBank· DDBJ | EFZ00589.2 EMBL· GenBank· DDBJ | Genomic DNA |