E9EFD6 · E9EFD6_METAQ
- ProteinPhosphatidylserine decarboxylase proenzyme 1, mitochondrial
- GenePSD1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids489 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine.
Catalytic activity
- a 1,2-diacyl-sn-glycero-3-phospho-L-serine + H+ = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + CO2
Cofactor
Note: Binds 1 pyruvoyl group covalently per subunit.
Pathway
Lipid metabolism.
Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 2/2.
Features
Showing features for active site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 171 | Charge relay system; for autoendoproteolytic cleavage activity | |||
Active site | 317 | Charge relay system; for autoendoproteolytic cleavage activity | |||
Site | 444-445 | Cleavage (non-hydrolytic); by autocatalysis | |||
Active site | 445 | Charge relay system; for autoendoproteolytic cleavage activity | |||
Active site | 445 | Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial inner membrane | |
Molecular Function | phosphatidylserine decarboxylase activity | |
Biological Process | phosphatidylethanolamine biosynthetic process | |
Biological Process | protein autoprocessing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphatidylserine decarboxylase proenzyme 1, mitochondrial
- EC number
- Cleaved into 2 chains
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Hypocreales > Clavicipitaceae > Metarhizium
Accessions
- Primary accessionE9EFD6
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Phosphatidylserine decarboxylase 1 alpha chain
Mitochondrion inner membrane ; Peripheral membrane protein
Note: Anchored to the mitochondrial inner membrane through its interaction with the integral membrane beta chain.
Phosphatidylserine decarboxylase 1 beta chain
Mitochondrion inner membrane ; Single-pass membrane protein
Features
Showing features for topological domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Topological domain | 1-39 | Mitochondrial matrix | |||
Topological domain | 59-489 | Mitochondrial intermembrane | |||
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_5023578308 | 1-444 | Phosphatidylserine decarboxylase 1 beta chain | ||
Modified residue | 445 | Pyruvic acid (Ser); by autocatalysis | |||
Chain | PRO_5023578309 | 445-489 | Phosphatidylserine decarboxylase 1 alpha chain | ||
Post-translational modification
Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The autoendoproteolytic cleavage occurs by a canonical serine protease mechanism, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain. During this reaction, the Ser that is part of the protease active site of the proenzyme becomes the pyruvoyl prosthetic group, which constitutes an essential element of the active site of the mature decarboxylase.
Keywords
- PTM
Interaction
Subunit
Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 103-129 | Disordered | |||
Sequence similarities
Belongs to the phosphatidylserine decarboxylase family. PSD-B subfamily. Eukaryotic type I sub-subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length489
- Mass (Da)54,043
- Last updated2011-04-05 v1
- Checksum637A980B1014B9B2
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
GL698579 EMBL· GenBank· DDBJ | EFY85389.1 EMBL· GenBank· DDBJ | Genomic DNA |