E8WMT9 · E8WMT9_GEOS8
- ProteinPyruvate carboxylase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1148 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Catalytic activity
- hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H+
Cofactor
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 121 | ATP (UniProtKB | ChEBI) | |||
Binding site | 205 | ATP (UniProtKB | ChEBI) | |||
Binding site | 240 | ATP (UniProtKB | ChEBI) | |||
Active site | 297 | ||||
Binding site | 545 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 617 | substrate | |||
Binding site | 714 | Mn2+ (UniProtKB | ChEBI); via carbamate group | |||
Binding site | 743 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 745 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 878 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | pyruvate carboxylase activity | |
Biological Process | gluconeogenesis | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate carboxylase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Thermodesulfobacteriota > Desulfuromonadia > Geobacterales > Geobacteraceae > Geobacter
Accessions
- Primary accessionE8WMT9
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 714 | N6-carboxylysine | |||
Modified residue | 1114 | N6-biotinyllysine | |||
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 5-458 | Biotin carboxylation | |||
Domain | 125-322 | ATP-grasp | |||
Domain | 536-804 | Pyruvate carboxyltransferase | |||
Region | 1059-1085 | Disordered | |||
Compositional bias | 1065-1079 | Basic and acidic residues | |||
Domain | 1073-1148 | Lipoyl-binding | |||
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,148
- Mass (Da)126,136
- Last updated2011-04-05 v1
- Checksum38B62F1943AF921F
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1065-1079 | Basic and acidic residues | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP002479 EMBL· GenBank· DDBJ | ADW11799.1 EMBL· GenBank· DDBJ | Genomic DNA |