E8WMT9 · E8WMT9_GEOS8

Function

function

Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.

Catalytic activity

Cofactor

biotin (UniProtKB | Rhea| CHEBI:57586 )

Features

Showing features for binding site, active site.

111481002003004005006007008009001,0001,100
Type
IDPosition(s)Description
Binding site121ATP (UniProtKB | ChEBI)
Binding site205ATP (UniProtKB | ChEBI)
Binding site240ATP (UniProtKB | ChEBI)
Active site297
Binding site545Mn2+ (UniProtKB | ChEBI)
Binding site617substrate
Binding site714Mn2+ (UniProtKB | ChEBI); via carbamate group
Binding site743Mn2+ (UniProtKB | ChEBI)
Binding site745Mn2+ (UniProtKB | ChEBI)
Binding site878substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular Functionpyruvate carboxylase activity
Biological Processgluconeogenesis
Biological Processpyruvate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pyruvate carboxylase
  • EC number

Gene names

    • Ordered locus names
      GM18_0310

Organism names

  • Taxonomic identifier
  • Strain
    • M18
  • Taxonomic lineage
    Bacteria > Thermodesulfobacteriota > Desulfuromonadia > Geobacterales > Geobacteraceae > Geobacter

Accessions

  • Primary accession
    E8WMT9

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue714N6-carboxylysine
Modified residue1114N6-biotinyllysine

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region, compositional bias.

Type
IDPosition(s)Description
Domain5-458Biotin carboxylation
Domain125-322ATP-grasp
Domain536-804Pyruvate carboxyltransferase
Region1059-1085Disordered
Compositional bias1065-1079Basic and acidic residues
Domain1073-1148Lipoyl-binding

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,148
  • Mass (Da)
    126,136
  • Last updated
    2011-04-05 v1
  • Checksum
    38B62F1943AF921F
MEARKFRKVMAANRGEIAIRIFRACTELGISTVAIYSEEDKLSLHRYKADEAYQVGKGKAPIDAYLGIDEIIALAKKREVDAIHPGYGFLAENAEFAEKCEANGIAFIGPTAEMQRALGDKVAARKVAKAAGVATVPGTEEPILHEEDALIFAKNYGYPIIIKAAAGGGGRGMRVATNQKELLEGLQSASSEAKAAFGDPSVFLERYLKNPKHIEVQVLGDAYGNLVHFFERDCSIQRRHQKVVEFAPSIALPGATRLALCTDALKIAQQVGYRNAGTVEFLLDEEGRYYFIEMNPRIQVEHTVTEMITGRNLVQAQILIAEGKRLADPEINIPNQGSIEMRGYAIQCRITTEDPTNNFAPDFGTLSTYRSSAGCGVRLDAGNAFTGAQITPHYDSLLVKVSSWGLTFAEAAHIMDRSLQEFRVRGVKTNIGFLENVITHPVFLAGECNTSFIDQHPELLVIPEKKDRANKVLNFLGDVIVNGSPGVAKPLRSAELIEADVPQIDPFTQKPKGTRDILREQGAEGLSKWVLEQKRLLLTDTTMRDAHQSLLATRVRTYDLLKIAEPTSHLASDLFSLELWGGATFDVSMRFLKEDPWQRLHALSEAIPNILFQMLLRGSNAVGYTNYPDNVVQRFVAQAAESGVDVFRVFDSLNWTRGMQVAMEAVQKSGKICEAAICYTGDISDPARTKYPLSYYVSMAKELEKMGAHILAIKDMAGLLKPYAGYQLVKALKEEIGIPVHLHTHDTSGNGGSLLLMAAQAGVDIVDAALSSISGLTSQPNLNALVATLKGTEFDPKVNERGLQQLANYWETVRDYYAPFESGLKSGTAEVYHHEIPGGQYSNYKPQVAGLGLIERWEECKEMYHKVNVLFGDVVKVTPSSKVVGDMAMFLVKNNLEPEDVFAPGADLAFPESVVGFFKGMIGQPYQGFPKELQKIILKGQEPITCRPGELLEPADFEKERATAEAKVGHPVNDEELMSYMMYPSVYVEYAKHRQEFSDVSVIPTPIFFYGLEPGQETSIELQPGKTLIVKLNAIGKTQPDGTKQIYFELNGNARSVTVRDQSVQSDESGHEKADKGNPKHVGAPMPGKVLKLNVKAGDAVKAGDILAVTEAMKMETNIKAKEDGTLAEVKCKEGGKVEKEELLFVMA

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1065-1079Basic and acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP002479
EMBL· GenBank· DDBJ
ADW11799.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp