E8MF12 · NAHK_BIFL2
- ProteinN-acetylhexosamine 1-kinase
- GenenahK
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids359 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Phosphorylates both N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) at similar rates. Involved in the lacto-N-biose I/galacto-N-biose (LNB/GNB) degradation pathway, which is important for host intestinal colonization by bifidobacteria. Also accepts GTP and ITP as phosphate donors. In vitro, can phosphorylate several GlcNAc and GalNAc derivatives.
Catalytic activity
- ATP + N-acetyl-D-hexosamine = ADP + H+ + N-acetyl-alpha-D-hexosamine 1-phosphate
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.118 mM | GlcNAc | |||||
0.065 mM | GalNAc | |||||
0.172 mM | ATP |
kcat is 1.21 sec-1 for GlcNAc. kcat is 0.752 sec-1 for GalNAc.
pH Dependence
Optimum pH is 8.5. Stable at pH 5.0 to 9.5 at 30 degrees Celsius.
Temperature Dependence
Optimum temperature is 40 degrees Celsius.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | homoserine kinase activity | |
Molecular Function | phosphotransferase activity, alcohol group as acceptor | |
Biological Process | carbohydrate metabolic process | |
Biological Process | threonine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameN-acetylhexosamine 1-kinase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Bifidobacteriales > Bifidobacteriaceae > Bifidobacterium
Accessions
- Primary accessionE8MF12
- Secondary accessions
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000424071 | 1-359 | N-acetylhexosamine 1-kinase | |||
Sequence: MTESNEDLFGIASHFALEGAVTGIEPYGDGHINTTYLVTTDGPRYILQQMNTSIFPDTVNLMRNVELVTSTLKAQGKETLDIVPTTSGATWAEIDGGAWRVYKFIEHTVSYNLVPNPDVFREAGSAFGDFQNFLSEFDASQLTETIAHFHDTPHRFEDFKAALAADKLGRAAACQPEIDFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDEKDLSKVHFSTELFRAYTEGFVGELRGSITAREAELLPFSGNLLTMECGMRFLADYLEGDIYFATKYPEHNLVRTRTQIKLVQEMEQKASETRAIVADIMEAAR |
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 21-359 | Protein kinase | ||||
Sequence: VTGIEPYGDGHINTTYLVTTDGPRYILQQMNTSIFPDTVNLMRNVELVTSTLKAQGKETLDIVPTTSGATWAEIDGGAWRVYKFIEHTVSYNLVPNPDVFREAGSAFGDFQNFLSEFDASQLTETIAHFHDTPHRFEDFKAALAADKLGRAAACQPEIDFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDEKDLSKVHFSTELFRAYTEGFVGELRGSITAREAELLPFSGNLLTMECGMRFLADYLEGDIYFATKYPEHNLVRTRTQIKLVQEMEQKASETRAIVADIMEAAR |
Sequence similarities
Belongs to the protein kinase superfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length359
- Mass (Da)39,903
- Last updated2011-04-05 v1
- ChecksumFBC9DD776E6BA5A3
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 7 | in Ref. 1; BAF73925 | ||||
Sequence: D → V |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB303839 EMBL· GenBank· DDBJ | BAF73925.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP010888 EMBL· GenBank· DDBJ | BAJ67289.1 EMBL· GenBank· DDBJ | Genomic DNA |