E7FR03 · E7FR03_9LACO

Function

function

Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

biotin (UniProtKB | Rhea| CHEBI:57586 )

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site116ATP (UniProtKB | ChEBI)
Binding site200ATP (UniProtKB | ChEBI)
Binding site235ATP (UniProtKB | ChEBI)
Active site291
Binding site537Mn2+ (UniProtKB | ChEBI)
Binding site609substrate
Binding site706Mn2+ (UniProtKB | ChEBI); via carbamate group
Binding site735Mn2+ (UniProtKB | ChEBI)
Binding site737Mn2+ (UniProtKB | ChEBI)
Binding site869substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular Functionpyruvate carboxylase activity
Biological Processgluconeogenesis
Biological Processpyruvate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pyruvate carboxylase
  • EC number

Gene names

    • Name
      pyc
    • ORF names
      HMPREF0542_11330

Organism names

Accessions

  • Primary accession
    E7FR03

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue706N6-carboxylysine
Modified residue1105N6-biotinyllysine

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1-451Biotin carboxylation
Domain120-316ATP-grasp
Domain528-796Pyruvate carboxyltransferase
Domain1064-1139Lipoyl-binding

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,142
  • Mass (Da)
    127,363
  • Last updated
    2011-04-05 v1
  • Checksum
    40A5F2CBB0F3D4DD
MKKILIANRGEIAIRIIRACHELHLKTVAIYAKEDEYGVHRFRADEAYLVGAGKKPIDAYLDMDDIIRIAKMTGADAIHPGYGFLSENEEFAQKCEDAGIIFIGPTVKQLQMFGDKVEAKEVAHAAGLETIPGTEDPVKSLEEVQNFAHKYGYPIMVKAAMGGGGRGMRVVNSDDELPDAYNRARSEAKQSFGDDELYVEKYLENPKHIEVQILGDKHGNVLHLFERDCSVQRRHQKVIEFAPSIILSDDRRKEICDAAVRLAKSIHYQNAGTVEFLVTDDAFYFIEVNPRIQVEHTITEMITEIDLVQSQILVAAGKDLFKDLHLPKQEDMGYRGCAIQCRITTEDSENNFMPDTGKIETYRSPGGFGVRLDGGNAYAGAVVTPYFDSLLVKVCVQAKTFGEAVDKMDRVLGEFRIRGVKTNIEFMRNVINNPVFRAGAAHTTFIDNTPELFKIKKSNNTNNQLLKYIGEVTVNGFPGVTRHDKLFVPDIKFGDKLNIEEGVVNAKSVFDAEGAEAAMQWVKDQNRVLLTDTSMRDAHQSLFATRMRTKDMAPVIDVYDKAFPHVFSAECWGGATFDVAYRFLNEDPWERLKLMRKKMPHTLLQMLLRGSNAVGYKNYPDNVLKAFIEKSAEDGIDVFRVFDSLNWVEQMENPLKYVRDAGKIAEGTMCYTGDVLSKEETKYTLDYYKHLAKQLVDCGSHIIGIKDMAGLLKPKAAFELVSALKEEVDVPIHLHTHDTTGNGISTYIAATKAGVDVIDVAASALSGTTSQPSMGSLYYALEGDVRQPQLDMENLEKVNNYWAGVKPFYQDFMNGINAPQPDIYQTEMPGGQYSNLKQQALALGIEDFDLVKAKYREVNKLLGDIVKVTPSSKVVGDCAIFMIQNNLDKNNILERGKVLDFPASVVNFFAGGLGQPYGGFPKKLQEVVLKGKKPITVRPGSLAEPVDFKAVKSELEQKIRREASEEEVLSYVLYPDVFLTYNQNSEKFGSMHSLATTVFYQGMRQGETIHVDFAPGRSVIIRLDSISDADEDGNRVVFFSLNGQNLRIIVHDKSKKAKVKAIPKAEPTNSNHIGATLSGSVTEVLVEKNQVVKKGEPLIVTEAMKMETTIKAPHDGKVTHIYVKAGDLLQSQDLLMELEPVK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
ACGS02000041
EMBL· GenBank· DDBJ
EFZ34390.1
EMBL· GenBank· DDBJ
Genomic DNA

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