E7FR03 · E7FR03_9LACO
- ProteinPyruvate carboxylase
- Genepyc
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1142 (go to sequence)
- Protein existencePredicted
- Annotation score2/5
Function
function
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Catalytic activity
- hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H+
Cofactor
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 116 | ATP (UniProtKB | ChEBI) | |||
Binding site | 200 | ATP (UniProtKB | ChEBI) | |||
Binding site | 235 | ATP (UniProtKB | ChEBI) | |||
Active site | 291 | ||||
Binding site | 537 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 609 | substrate | |||
Binding site | 706 | Mn2+ (UniProtKB | ChEBI); via carbamate group | |||
Binding site | 735 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 737 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 869 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | pyruvate carboxylase activity | |
Biological Process | gluconeogenesis | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate carboxylase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Lactobacillaceae > Ligilactobacillus
Accessions
- Primary accessionE7FR03
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 706 | N6-carboxylysine | |||
Modified residue | 1105 | N6-biotinyllysine | |||
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 1-451 | Biotin carboxylation | |||
Domain | 120-316 | ATP-grasp | |||
Domain | 528-796 | Pyruvate carboxyltransferase | |||
Domain | 1064-1139 | Lipoyl-binding | |||
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,142
- Mass (Da)127,363
- Last updated2011-04-05 v1
- Checksum40A5F2CBB0F3D4DD
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
ACGS02000041 EMBL· GenBank· DDBJ | EFZ34390.1 EMBL· GenBank· DDBJ | Genomic DNA |