E7F4V8 · PEX2_DANRE

Function

function

E3 ubiquitin-protein ligase component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling (By similarity).
The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane. PEX2 also regulates peroxisome organization by acting as a E3 ubiquitin-protein ligase (By similarity).

Catalytic activity

  • [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-S-ubiquitinyl-L-cysteine.
    EC:2.3.2.36 (UniProtKB | ENZYME | Rhea)
  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.
    EC:2.3.2.27 (UniProtKB | ENZYME | Rhea)

Pathway

Protein modification; protein ubiquitination.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site252Zn2+ 1 (UniProtKB | ChEBI)
Binding site255Zn2+ 1 (UniProtKB | ChEBI)
Binding site267Zn2+ 2 (UniProtKB | ChEBI)
Binding site269Zn2+ 2 (UniProtKB | ChEBI)
Binding site272Zn2+ 1 (UniProtKB | ChEBI)
Binding site275Zn2+ 1 (UniProtKB | ChEBI)
Binding site288Zn2+ 2 (UniProtKB | ChEBI)
Binding site291Zn2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentperoxisomal membrane
Molecular Functionubiquitin protein ligase activity
Molecular Functionzinc ion binding
Biological Processperoxisome organization
Biological Processpexophagy
Biological Processprotein import into peroxisome matrix, receptor recycling
Biological Processprotein ubiquitination
Biological Processresponse to amino acid starvation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Peroxisome biogenesis factor 2
  • EC number
  • Alternative names
    • Peroxin-2

Gene names

    • Name
      pex2

Organism names

  • Taxonomic identifier
  • Strain
    • Tuebingen
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio

Accessions

  • Primary accession
    E7F4V8
  • Secondary accessions
    • A0A8M9PUF8
    • E9QD16

Proteomes

Organism-specific databases

Subcellular Location

Peroxisome membrane
; Multi-pass membrane protein

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-22Peroxisomal matrix
Transmembrane23-49Helical; Name=TM1
Topological domain50-55Cytoplasmic
Transmembrane56-81Helical; Name=TM2
Topological domain82-105Peroxisomal matrix
Transmembrane106-132Helical; Name=TM3
Topological domain133-141Cytoplasmic
Transmembrane142-168Helical; Name=TM4
Topological domain169-195Peroxisomal matrix
Transmembrane196-219Helical; Name=TM5
Topological domain220-312Cytoplasmic

Keywords

Phenotypes & Variants

Disruption phenotype

Mutant fishes show a loss of peroxisomal structure, associated with high-mortality (PubMed:34016526).
Defects are similar to human Zellweger syndrome (ZS), characterized by developmental abnormalities in many organs, organ-specific accumulation and reduction of distinct fatty acid species, such as an accumulation of ultra-very-long-chain polyunsaturated fatty acids (ultra-VLC-PUFAs) (PubMed:34016526).

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004569831-312Peroxisome biogenesis factor 2

Proteomic databases

Expression

Gene expression databases

Interaction

Subunit

Component of the PEX2-PEX10-PEX12 retrotranslocation channel.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for zinc finger.

TypeIDPosition(s)Description
Zinc finger252-292RING-type

Domain

The three subunits of the retrotranslocation channel (PEX2, PEX10 and PEX12) coassemble in the membrane into a channel with an open 10 Angstrom pore. The RING-type zinc-fingers that catalyze PEX5 receptor ubiquitination are positioned above the pore on the cytosolic side of the complex.

Sequence similarities

Belongs to the pex2/pex10/pex12 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    312
  • Mass (Da)
    35,131
  • Last updated
    2011-03-08 v1
  • Checksum
    8F905A55DE887D17
MAGAGGDKPFAKAGPSPLSRVLRISQLDAFELDGALEQLVWSQFTQCFQHFKPGILTPVEPELKALLQLLLWRFTIYSNSATVGQSLLNIRYKNALIPGQKYRPMSRPQKFWFALLTVGEKWFRERSHSLFLNHPAESNARKARKVLSILLGLTKAASLVNFLLFLQRGTFPTLTERLLGVQPVFSRPQGPRDINFQYLNRELLWHGFAEFLIFLLPLINVWKLKAGVSALFSPLSDLTGTQSSEETHLTECAICGEWPTMPHSIGCKHVFCYYCVKSNVIADIYFTCPKCGAETGQIEPVRLQVGTELLQS

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A9B7QDH4A0A9B7QDH4_DANREpex2312

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CR361560
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

Disclaimer

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