E7BQV0 · NPR1_MALHU
- ProteinBTB/POZ domain and ankyrin repeat-containing protein NPR1
- GeneNPR1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids586 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Salicylic acid (SA)-binding substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).
Transcription cofactor that represses gene expression in the absence of salicylic acid (SA), when attached to negative cis-elements (W-box) with WRKY transcription factors, but stimulates gene expression upon activation by SA, when sumoylated and attached to positive cis-elements (as-1) with TGA transcription factors, thus confering immunity through a series of gene regulations ending in a significant increase in antimicrobial and defense genes expression (By similarity).
Probable component of the salicylic acid (SA) defense signaling pathway and pathogen-induced systemic acquired resistance (SAR) (Probable). May be involved in disease resistance against fungal pathogens (Probable). May be involved in tolerance to salt and osmotic stresses (PubMed:24407603).
Transcription cofactor that represses gene expression in the absence of salicylic acid (SA), when attached to negative cis-elements (W-box) with WRKY transcription factors, but stimulates gene expression upon activation by SA, when sumoylated and attached to positive cis-elements (as-1) with TGA transcription factors, thus confering immunity through a series of gene regulations ending in a significant increase in antimicrobial and defense genes expression (By similarity).
Probable component of the salicylic acid (SA) defense signaling pathway and pathogen-induced systemic acquired resistance (SAR) (Probable). May be involved in disease resistance against fungal pathogens (Probable). May be involved in tolerance to salt and osmotic stresses (PubMed:24407603).
Pathway
Protein modification; protein ubiquitination.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nuclear body | |
Molecular Function | metal ion binding | |
Biological Process | defense response | |
Biological Process | regulation of jasmonic acid mediated signaling pathway | |
Biological Process | regulation of salicylic acid mediated signaling pathway | |
Biological Process | response to salt stress | |
Biological Process | response to water deprivation | |
Biological Process | systemic acquired resistance, salicylic acid mediated signaling pathway |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBTB/POZ domain and ankyrin repeat-containing protein NPR1
- Short namesMhNPR1
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Rosales > Rosaceae > Amygdaloideae > Maleae > Malus
Accessions
- Primary accessionE7BQV0
- Secondary accessions
Subcellular Location
UniProt Annotation
GO Annotation
Note: Accumulation in nucleus after induction by salicylic acid (SA) treatment or after pathogen infection.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000447479 | 1-586 | BTB/POZ domain and ankyrin repeat-containing protein NPR1 | |||
Sequence: MAHSAEPSSSLSFTSSPHLSNGSISHNLSCSGSESVPSLEVISLSKLSSSLEQLLIDPGCDYSDADIVVEGIPVGVHRCILASRSGFFRELFKREKGSSGKEDRPKYCMSDFLPYGDVGYEAFLVFLSYVYTGKLKPSPVEVSTCVHNVCAHDACRPAINFVVELMYAASIFQMPDLVSIFERRLLNFVGKALSDNVVPILLVAFHCQLNQLIDQCVDRVARSDIDDISLEKGLPDEVVKKIKILRRNYQQDSDPNLPPADPLHEKRIRRIHKALDSDDVELVKLLLTESNITLDEANALHYAAAYCDPKVVTEVLALGLADVNLRNSRGYTALHIAVMRKEPSIIVLLLTKGARASELTSDGQSAVSICRRLTRPKDYHSKTEQGQEANKDRICIDVLEREMRRNPMAGDASISSQIMPDDLHMELLNLENRVALARLFFPAEAKLAMVIAHAETSEFAAPSSSKGSSGNLMEVDLNETPTVQNKRLHSRLEALMKTVRLGRCYFPHCSEVLDKFIDDDLPHLFYLEPGSSDEQKVKRRRFMELKEEVQKAFDKDKAECNLSGLSSSSSTTSPEKIGANQKVREP |
Structure
Family & Domains
Features
Showing features for domain, zinc finger, repeat, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 63-139 | BTB | ||||
Sequence: SDADIVVEGIPVGVHRCILASRSGFFRELFKREKGSSGKEDRPKYCMSDFLPYGDVGYEAFLVFLSYVYTGKLKPSP | ||||||
Zinc finger | 140-156 | C2HC-type | ||||
Sequence: VEVSTCVHNVCAHDACR | ||||||
Repeat | 266-296 | ANK 1 | ||||
Sequence: KRIRRIHKALDSDDVELVKLLLTESNITLDE | ||||||
Repeat | 298-325 | ANK 2 | ||||
Sequence: NALHYAAAYCDPKVVTEVLALGLADVNL | ||||||
Repeat | 329-358 | ANK 3 | ||||
Sequence: RGYTALHIAVMRKEPSIIVLLLTKGARASE | ||||||
Region | 388-522 | Salicylic acid-binding core (SBC) | ||||
Sequence: EANKDRICIDVLEREMRRNPMAGDASISSQIMPDDLHMELLNLENRVALARLFFPAEAKLAMVIAHAETSEFAAPSSSKGSSGNLMEVDLNETPTVQNKRLHSRLEALMKTVRLGRCYFPHCSEVLDKFIDDDLP | ||||||
Compositional bias | 561-578 | Polar residues | ||||
Sequence: NLSGLSSSSSTTSPEKIG | ||||||
Region | 561-586 | Disordered | ||||
Sequence: NLSGLSSSSSTTSPEKIGANQKVREP |
Domain
The BTB/POZ domain mediates the interaction with some component of ubiquitin ligase complexes.
Sequence similarities
Belongs to the plant 'ANKYRIN-BTB/POZ' family. 'NPR1-like' subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length586
- Mass (Da)65,083
- Last updated2019-07-03 v2
- Checksum11C480E7AA714DD3
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 93 | in Ref. 1; ACU78081 | ||||
Sequence: K → R | ||||||
Sequence conflict | 163-166 | in Ref. 1; ADP95762 | ||||
Sequence: VELM → EELT | ||||||
Sequence conflict | 226 | in Ref. 1; ACU78081 | ||||
Sequence: D → N | ||||||
Sequence conflict | 258 | in Ref. 1; ACU78081 | ||||
Sequence: P → S | ||||||
Sequence conflict | 285 | in Ref. 1; ACU78081 | ||||
Sequence: L → P | ||||||
Sequence conflict | 333 | in Ref. 1; ACU78081 | ||||
Sequence: A → V | ||||||
Sequence conflict | 383 | in Ref. 1; ACU78081 | ||||
Sequence: T → A | ||||||
Sequence conflict | 485 | in Ref. 1; ACU78081 | ||||
Sequence: N → S | ||||||
Compositional bias | 561-578 | Polar residues | ||||
Sequence: NLSGLSSSSSTTSPEKIG | ||||||
Sequence conflict | 566 | in Ref. 1; ACU78081 | ||||
Sequence: S → P | ||||||
Sequence conflict | 575 | in Ref. 1; ACU78081 | ||||
Sequence: E → K |