E6Z0R3 · VBHT_BARSR

Function

function

Toxic component of type II toxin-antitoxin (TA) system VbhT-VbhA. Adenylyltransferase involved in virulence by mediating the addition of adenosine 5'-monophosphate (AMP) to specific residue of host GTPases. The resulting AMPylation affects GTPases, impairing actin assembly in infected cells.

Miscellaneous

Defined as class I fido-domain containing proteins, in which the inhibitory helix is provided by an interacting antitoxin (VbhA).

Catalytic activity

Activity regulation

Adenylyltransferase activity is inhibited by antitoxin VbhA; which acts by competing with ATP-binding at Arg-147 and prevents productive ATP-binding.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site85-88ATP (UniProtKB | ChEBI)
Binding site133-136ATP (UniProtKB | ChEBI)
Binding site140-147ATP (UniProtKB | ChEBI)
Binding site175ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionAMPylase activity
Molecular FunctionATP binding
Molecular Functionmagnesium ion binding
Molecular Functionprotein homodimerization activity
Biological Processprotein adenylylation
Biological Processregulation of cell division

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Protein adenylyltransferase VbhT
  • EC number
  • Alternative names
    • AMPylator VbhT
    • Toxin VbhT

Gene names

    • Name
      vbhT
    • ORF names
      B11C_100026

Organism names

Accessions

  • Primary accession
    E6Z0R3

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis136Abolishes adenylyltransferase activity.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004175491-478Protein adenylyltransferase VbhT

Interaction

Subunit

Homodimer. Interacts with VbhA.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntAct
BINARY E6Z0R3B11C_100027 E6Z0R42EBI-15965345, EBI-15965363

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain55-200Fido

Domain

The fido domain mediates the adenylyltransferase activity.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    478
  • Mass (Da)
    55,379
  • Last updated
    2011-03-08 v1
  • MD5 Checksum
    7CB3019BE6D201BBEF1892D8632772FC
MRKYEGSNDPYTDPETGVMYNLLGIKDQARLERVESAFAYIRSFELGRTSISGKFDLDHMKKIHKKLFGDVYEWAGKTRLVDIVKDNSKFAHYTQIESYAPQITQQLAREQHLRGLDANEFSQRAGYYMGELNALHPFREGNGRTLREFIWQLAREAGYHIDWDRVERQEMTRASIESYYGNSDLMSALIRRNLTEFTVNRRVDVSQGINERVLSHIDIDKEWPQKGFNIAIQTTQQAPYLSSYTDTSNLEEKAQNALRNEQSYVDTFKELNDHLKTIYKDPQAAALKIEQTILAGKGDKLPDILAKAPNKVGELRGSDRLIDKLKSAGKERKAALYNVPLAISTIRRLQSFYKNSYEKHMDKLTREREQLKVEVPSLSQEAVAYMKNVEVGRNNYSKIPENINKEFVQLESALNRRFGKDVIYKRNFNLSKEIASKQTYDKKLVNELQTAIKFLQQRHIQKQNNLAITRTPSKGITR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FN645515
EMBL· GenBank· DDBJ
CBI82701.1
EMBL· GenBank· DDBJ
Genomic DNA

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