E6Z0R3 · VBHT_BARSR
- ProteinProtein adenylyltransferase VbhT
- GenevbhT
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids478 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Toxic component of type II toxin-antitoxin (TA) system VbhT-VbhA. Adenylyltransferase involved in virulence by mediating the addition of adenosine 5'-monophosphate (AMP) to specific residue of host GTPases. The resulting AMPylation affects GTPases, impairing actin assembly in infected cells.
Miscellaneous
Defined as class I fido-domain containing proteins, in which the inhibitory helix is provided by an interacting antitoxin (VbhA).
Catalytic activity
- L-tyrosyl-[protein] + ATP = O-(5'-adenylyl)-L-tyrosyl-[protein] + diphosphate
Activity regulation
Adenylyltransferase activity is inhibited by antitoxin VbhA; which acts by competing with ATP-binding at Arg-147 and prevents productive ATP-binding.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 85-88 | ATP (UniProtKB | ChEBI) | |||
Binding site | 133-136 | ATP (UniProtKB | ChEBI) | |||
Binding site | 140-147 | ATP (UniProtKB | ChEBI) | |||
Binding site | 175 | ATP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | AMPylase activity | |
Molecular Function | ATP binding | |
Molecular Function | magnesium ion binding | |
Molecular Function | protein homodimerization activity | |
Biological Process | protein adenylylation | |
Biological Process | regulation of cell division |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein adenylyltransferase VbhT
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Bartonellaceae > Bartonella
Accessions
- Primary accessionE6Z0R3
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 136 | Abolishes adenylyltransferase activity. | |||
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000417549 | 1-478 | Protein adenylyltransferase VbhT | ||
Interaction
Subunit
Homodimer. Interacts with VbhA.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
BINARY | E6Z0R3 | B11C_100027 E6Z0R4 | 2 | EBI-15965345, EBI-15965363 |
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length478
- Mass (Da)55,379
- Last updated2011-03-08 v1
- MD5 Checksum7CB3019BE6D201BBEF1892D8632772FC
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FN645515 EMBL· GenBank· DDBJ | CBI82701.1 EMBL· GenBank· DDBJ | Genomic DNA |