E6LHV7 · CAS10_ENTI1
- ProteinCRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)
- Genecas10
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids755 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). The type III-A Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities. In a heterologous host the appropriately targeted Csm effector complex prevents growth of dsDNA phage phiNM1-gamma6.
ssDNase activity is stimulated in the ternary Csm effector complex; binding of cognate target RNA activates the ssDNase, as the target RNA is degraded ssDNA activity decreases.
This subunit is a single-strand-specific deoxyribonuclease (ssDNase) which digests both linear and circular ssDNA; it has both exo- and endonuclease activity.
When associated with the ternary Csm effector complex (the crRNA, Cas proteins and a cognate target ssRNA) synthesizes cyclic oligoadenylates (cOA) from ATP, producing (mostly) cyclic hexaadenylate (cA6). cA6 synthesis occurs in the Csm effector complex and requires cognate target RNA and ATP; other NTPs are not incorporated. cOAs are second messengers that induce an antiviral state important for defense against invading nucleic acids.
Miscellaneous
Encoded in a type III-A CRISPR locus.
Catalytic activity
- 6 ATP = cyclic hexaadenylate + 6 diphosphate
Cofactor
Note: Synthesis of cA6 requires Mg2+.
Activity regulation
Synthesis of cA6 is inhibited by EDTA.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | endonuclease activity | |
Molecular Function | exonuclease activity | |
Molecular Function | RNA binding | |
Molecular Function | transferase activity | |
Biological Process | defense response to virus |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)
- EC number
- Short namesssDNase Cas10
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Enterococcaceae > Enterococcus
Accessions
- Primary accessionE6LHV7
Proteomes
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 14-15 | Wild-type synthesis of the cA6 activator. | ||||
Sequence: HD → AN | ||||||
Mutagenesis | 584-585 | No longer synthesizes the cA6 activator. | ||||
Sequence: DD → AA |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000446111 | 1-755 | CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A) | |||
Sequence: MNKKLELMYGSLLHDIGKIVYRSNSVDFAKGTHSKIGSQFLNKFKPFQLSGIVDSVSYHHYKELASSSLLDDSVAYITYIADNIASGTDRRASEGDYEGEGNRQRFDKRAPLASIFNVVNSETKGLANYTYSFEKEQVYRYPTDAKKEYTSSQYAALVNKMTDDLSNKLKVGPDSFSSLLQWTESLWSYIPSSTDTNQVMDVSLYDHSKITCAIASCIYDYLTEMNCVNYRKELFSPYEKTKQFYQEDVFLLVSLDMSGIQDFIYNISGSKALKSLRSRSFYLETMLESLVDDLLSDLELSRANLLYTGGGHAYLLLPNTERARDVLASFEGEMKEWFIKIFKTDLSVAIAYKACTGEDLMNSNGTYSDLWQTVSRKLSDKKAHKYSLNEIKLFNSTIHAGTQECKECLRSDIDISEDSLCKICEGIIAISNDLRDYSFFVVSPEGKVPLPRNRYLSVENQDGAERKIKMNKETRIYSKNQPFVGKQLVTNLWMCDYDFSTLNPETKKQGIASYVNREVGIPRLGVLRADIDNLGTTFIKGIPEQYRSISRTATLSRQLSMFFKFELSNILKGARISVIYSGGDDLFLIGAWDDVISKALVLRKAFTRFSAGKLTFSAGIGMYPVKYPISKMASETGVLEDLAKRGEKNQVALWNDSKVFGWSQLEEQILKEKMIPLQEALTNSQEHGKSFLYKMLELLRNEDQINIARLAYLLARSSLSEELTQSIFAWSQNKQQKVELITAIEYLVYQIREAD |
Interaction
Subunit
Part of the Csm effector complex that includes Cas10, Csm2, Csm3, Csm4 and Csm5.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-87 | HD domain | ||||
Sequence: MNKKLELMYGSLLHDIGKIVYRSNSVDFAKGTHSKIGSQFLNKFKPFQLSGIVDSVSYHHYKELASSSLLDDSVAYITYIADNIASG | ||||||
Domain | 522-656 | GGDEF | ||||
Sequence: PRLGVLRADIDNLGTTFIKGIPEQYRSISRTATLSRQLSMFFKFELSNILKGARISVIYSGGDDLFLIGAWDDVISKALVLRKAFTRFSAGKLTFSAGIGMYPVKYPISKMASETGVLEDLAKRGEKNQVALWND |
Domain
The N-terminal HD domain has ssDNase activity. The C-terminal GGDEF domain has the cOA synthesis activity.
Sequence similarities
Belongs to the CRISPR-associated Cas10/Csm1 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length755
- Mass (Da)85,841
- Last updated2011-03-08 v1
- Checksum89707E26C2128EB7
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AEPV01000074 EMBL· GenBank· DDBJ | EFU73217.1 EMBL· GenBank· DDBJ | Genomic DNA |