E6LHV7 · CAS10_ENTI1

Function

function

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). The type III-A Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities. In a heterologous host the appropriately targeted Csm effector complex prevents growth of dsDNA phage phiNM1-gamma6.
ssDNase activity is stimulated in the ternary Csm effector complex; binding of cognate target RNA activates the ssDNase, as the target RNA is degraded ssDNA activity decreases.
This subunit is a single-strand-specific deoxyribonuclease (ssDNase) which digests both linear and circular ssDNA; it has both exo- and endonuclease activity.
When associated with the ternary Csm effector complex (the crRNA, Cas proteins and a cognate target ssRNA) synthesizes cyclic oligoadenylates (cOA) from ATP, producing (mostly) cyclic hexaadenylate (cA6). cA6 synthesis occurs in the Csm effector complex and requires cognate target RNA and ATP; other NTPs are not incorporated. cOAs are second messengers that induce an antiviral state important for defense against invading nucleic acids.

Miscellaneous

Encoded in a type III-A CRISPR locus.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Synthesis of cA6 requires Mg2+.

Activity regulation

Synthesis of cA6 is inhibited by EDTA.

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functionendonuclease activity
Molecular Functionexonuclease activity
Molecular FunctionRNA binding
Molecular Functiontransferase activity
Biological Processdefense response to virus

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)
  • EC number
  • Short names
    ssDNase Cas10
  • Alternative names
    • Cyclic oligoadenylate synthase
      (EC:2.7.7.-
      ) . EC:2.7.7.- (UniProtKB | ENZYME | Rhea)
    • EiCas10

Gene names

    • Name
      cas10
    • Synonyms
      csm1
    • ORF names
      HMPREF9088_1947

Organism names

Accessions

  • Primary accession
    E6LHV7

Proteomes

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis14-15Wild-type synthesis of the cA6 activator.
Mutagenesis584-585No longer synthesizes the cA6 activator.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004461111-755CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)

Interaction

Subunit

Part of the Csm effector complex that includes Cas10, Csm2, Csm3, Csm4 and Csm5.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-87HD domain
Domain522-656GGDEF

Domain

The N-terminal HD domain has ssDNase activity. The C-terminal GGDEF domain has the cOA synthesis activity.

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    755
  • Mass (Da)
    85,841
  • Last updated
    2011-03-08 v1
  • Checksum
    89707E26C2128EB7
MNKKLELMYGSLLHDIGKIVYRSNSVDFAKGTHSKIGSQFLNKFKPFQLSGIVDSVSYHHYKELASSSLLDDSVAYITYIADNIASGTDRRASEGDYEGEGNRQRFDKRAPLASIFNVVNSETKGLANYTYSFEKEQVYRYPTDAKKEYTSSQYAALVNKMTDDLSNKLKVGPDSFSSLLQWTESLWSYIPSSTDTNQVMDVSLYDHSKITCAIASCIYDYLTEMNCVNYRKELFSPYEKTKQFYQEDVFLLVSLDMSGIQDFIYNISGSKALKSLRSRSFYLETMLESLVDDLLSDLELSRANLLYTGGGHAYLLLPNTERARDVLASFEGEMKEWFIKIFKTDLSVAIAYKACTGEDLMNSNGTYSDLWQTVSRKLSDKKAHKYSLNEIKLFNSTIHAGTQECKECLRSDIDISEDSLCKICEGIIAISNDLRDYSFFVVSPEGKVPLPRNRYLSVENQDGAERKIKMNKETRIYSKNQPFVGKQLVTNLWMCDYDFSTLNPETKKQGIASYVNREVGIPRLGVLRADIDNLGTTFIKGIPEQYRSISRTATLSRQLSMFFKFELSNILKGARISVIYSGGDDLFLIGAWDDVISKALVLRKAFTRFSAGKLTFSAGIGMYPVKYPISKMASETGVLEDLAKRGEKNQVALWNDSKVFGWSQLEEQILKEKMIPLQEALTNSQEHGKSFLYKMLELLRNEDQINIARLAYLLARSSLSEELTQSIFAWSQNKQQKVELITAIEYLVYQIREAD

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AEPV01000074
EMBL· GenBank· DDBJ
EFU73217.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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