E3ZRV8 · E3ZRV8_LISSE
- Protein3-methyl-2-oxobutanoate hydroxymethyltransferase
- GenepanB
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids277 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.
Catalytic activity
- (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = (6S)-5,6,7,8-tetrahydrofolate + 2-dehydropantoate
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Pathway
Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 43 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 43-44 | 3-methyl-2-oxobutanoate (UniProtKB | ChEBI) | ||||
Sequence: DS | ||||||
Binding site | 82 | 3-methyl-2-oxobutanoate (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 82 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 112 | 3-methyl-2-oxobutanoate (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 114 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Active site | 181 | Proton acceptor | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 3-methyl-2-oxobutanoate hydroxymethyltransferase activity | |
Molecular Function | metal ion binding | |
Molecular Function | methyltransferase activity | |
Biological Process | methylation | |
Biological Process | pantothenate biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3-methyl-2-oxobutanoate hydroxymethyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Listeriaceae > Listeria
Accessions
- Primary accessionE3ZRV8
Proteomes
Subcellular Location
Interaction
Subunit
Homodecamer; pentamer of dimers.
Structure
Sequence
- Sequence statusComplete
- Length277
- Mass (Da)30,014
- Last updated2011-02-08 v1
- ChecksumBF170CB45372EC5E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
ADXJ01000771 EMBL· GenBank· DDBJ | EFR99638.1 EMBL· GenBank· DDBJ | Genomic DNA |