E3YBA4 · MBCTN_METTR
- ProteinMethanobactin mb-OB3b
- GenembnA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Chalkophore involved in scavenging, uptake and suppression of toxicity of copper. Each apo-methanobactin (apo-mb) complexes 1 Cu2+ or Cu1+ ion to form Cu1+-mb (Cu-mb) which is then taken up by the cell. Enhances growth rate in the presence of copper and reduces growth lag upon exposition to elevated levels of copper. Cu-mb contributes to the switchover from soluble methane monooxygenase (sMMO) to the membrane-bound particulate MMO (pMMO) by inducing transcription of pMMO subunit A. It also stimulates the enzymatic activity of pMMO. In the absence of copper, binds other metal ions, like Zn2+, Ag1+, Au3+, Co2+, Cd2+, Fe3+, Hg2+, Mn2+, Ni2+, Pb2+ or U6+, but not Ba2+, Ca2+, La2+, Mg2+ or Sr2+. Uptake is an active process, which may involve TonB-dependent transporters, and as such does not involve porins. Cu-Mb can be taken up by other methanotrophic bacteria but not by E.coli. Has Cu-dependent superoxide dismutase-like activity. Shows reductant-dependent oxidase and hydrogen peroxide reductase activities. Reduces copper-levels in liver in a rat model of Wilson disease.
Catalytic activity
- 2 H+ + 2 superoxide = H2O2 + O2
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | extracellular region | |
Molecular Function | metal ion binding | |
Molecular Function | peroxidase activity | |
Molecular Function | superoxide dismutase activity | |
Biological Process | copper ion transport |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameMethanobactin mb-OB3b
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Methylocystaceae > Methylosinus
Accessions
- Primary accessionE3YBA4
PTM/Processing
Features
Showing features for propeptide, cross-link, peptide, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Propeptide | PRO_0000415151 | 1-19 | ||||
Sequence: MTVKIAQKKVLPVIGRAAA | ||||||
Cross-link | 20↔21 | 2-(3-methylbutanoyl)-5-hydroxyoxazole-4-carbothionic acid (Leu-Cys) | ||||
Sequence: LC | ||||||
Peptide | PRO_0000415152 | 20-30 | Methanobactin mb-OB3b | |||
Sequence: LCGSCYPCSCM | ||||||
Disulfide bond | 24↔29 | |||||
Sequence: CYPCSC | ||||||
Cross-link | 26↔27 | Proline 5-hydroxy-oxazole-4-carbothionic acid (Pro-Cys) | ||||
Sequence: PC |
Keywords
- PTM
Expression
Induction
By low copper concentrations.
Interaction
Subunit
Monomer. In the absence of copper, may exist as a dimer or an oligomer.
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length30
- Mass (Da)3,143
- Last updated2011-02-08 v1
- Checksum80ACD598389EECE5
Mass Spectrometry
Keywords
- Technical term