E3YBA4 · MBCTN_METTR

Function

function

Chalkophore involved in scavenging, uptake and suppression of toxicity of copper. Each apo-methanobactin (apo-mb) complexes 1 Cu2+ or Cu1+ ion to form Cu1+-mb (Cu-mb) which is then taken up by the cell. Enhances growth rate in the presence of copper and reduces growth lag upon exposition to elevated levels of copper. Cu-mb contributes to the switchover from soluble methane monooxygenase (sMMO) to the membrane-bound particulate MMO (pMMO) by inducing transcription of pMMO subunit A. It also stimulates the enzymatic activity of pMMO. In the absence of copper, binds other metal ions, like Zn2+, Ag1+, Au3+, Co2+, Cd2+, Fe3+, Hg2+, Mn2+, Ni2+, Pb2+ or U6+, but not Ba2+, Ca2+, La2+, Mg2+ or Sr2+. Uptake is an active process, which may involve TonB-dependent transporters, and as such does not involve porins. Cu-Mb can be taken up by other methanotrophic bacteria but not by E.coli. Has Cu-dependent superoxide dismutase-like activity. Shows reductant-dependent oxidase and hydrogen peroxide reductase activities. Reduces copper-levels in liver in a rat model of Wilson disease.

Caution

The rearrangement modifications of the Cys residues involved in the cross-links has not been described and the structure may be subject to revision.

Catalytic activity

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site21Cu2+ (UniProtKB | ChEBI)
Binding site27Cu2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentextracellular region
Molecular Functionmetal ion binding
Molecular Functionperoxidase activity
Molecular Functionsuperoxide dismutase activity
Biological Processcopper ion transport

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Methanobactin mb-OB3b
  • Alternative names
    • Copper-binding compound
      (CBC
      )
    • Hydrogen peroxide reductase
      (EC:1.11.1.-
      ) . EC:1.11.1.- (UniProtKB | ENZYME | Rhea)
    • Superoxide dismutase
      (EC:1.15.1.1
      ) . EC:1.15.1.1 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      mbnA
    • ORF names
      MettrDRAFT_4473

Organism names

  • Taxonomic identifier
  • Strain
    • ATCC 35070 / NCIMB 11131 / ACM 3311 / OB3b
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Methylocystaceae > Methylosinus

Accessions

  • Primary accession
    E3YBA4

Subcellular Location

Note: Secreted as apo-mb, uptake into the cytoplasm in complex with copper as Cu-mb. In the cytoplasm, Cu-mb is associated with the cell membrane.

Keywords

PTM/Processing

Features

Showing features for propeptide, cross-link, peptide, disulfide bond.

TypeIDPosition(s)Description
PropeptidePRO_00004151511-19
Cross-link20↔212-(3-methylbutanoyl)-5-hydroxyoxazole-4-carbothionic acid (Leu-Cys)
PeptidePRO_000041515220-30Methanobactin mb-OB3b
Disulfide bond24↔29
Cross-link26↔27Proline 5-hydroxy-oxazole-4-carbothionic acid (Pro-Cys)

Keywords

Expression

Induction

By low copper concentrations.

Interaction

Subunit

Monomer. In the absence of copper, may exist as a dimer or an oligomer.

Family & Domains

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    30
  • Mass (Da)
    3,143
  • Last updated
    2011-02-08 v1
  • Checksum
    80ACD598389EECE5
MTVKIAQKKVLPVIGRAAALCGSCYPCSCM

Mass Spectrometry

Molecular mass is 1,217 Da. Determined by MALDI. In complex with copper.
Molecular mass is 1,217.46 Da. Determined by MALDI. In complex with copper.
Molecular mass is 1,217.2 Da. Determined by MALDI. In complex with copper.
Molecular mass is 1,217 Da. Determined by Electrospray. In complex with copper.
Molecular mass is 1,217.18 Da. Determined by Electrospray. In complex with copper.
Molecular mass is 1,154 Da. Determined by Electrospray. Without copper.
Molecular mass is 1,154.46 Da. Determined by MALDI. Without copper.
Molecular mass is 1,154 Da. Determined by MALDI. Without copper.
Molecular mass is 1,154.24 Da. Determined by Electrospray. Without copper.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
ADVE01000073
EMBL· GenBank· DDBJ
EFS20503.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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