E3PJ88 · ASPS2_ECOH1

Function

function

Part of a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of folded proteins across the outer membrane (Probable). Required for correct assembly of the type II secretion system-beta (T2SS-beta), for localization of GspD-beta to the cell outer membrane and for export of a labile enterotoxin by T2SS-beta (PubMed:22585966).
Each AspS2 binds to 2 GspD2 subunits and may clamp the monomers together, stabilizing structure and accelerating its assembly (PubMed:29632366).

Miscellaneous

Encoded in a type II secretion system (T2SS-beta); this strain encodes 2 T2SS but only this one (beta) is expressed under standard laboratory conditions.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcell outer membrane

Names & Taxonomy

Protein names

  • Recommended name
    Pilotin AspS 2
  • Alternative names
    • Type II secretion system-beta protein GspS-beta

Gene names

    • Name
      gspS2
    • Synonyms
      aspS
      , gspS-beta
      , yghG
    • Ordered locus names
      ETEC_3239

Organism names

Accessions

  • Primary accession
    E3PJ88

Subcellular Location

Cell outer membrane
; Lipid-anchor
Note: Protein can also be located in the cell inner membrane.

Keywords

Phenotypes & Variants

Disruption phenotype

Severely disrupts assembly and function of T2SS-beta, mislocalization of GspD-beta to the cell inner membrane.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis25Precursor protein is not processed.
Mutagenesis26-27Protein is found almost exclusively in the inner membrane, does not restore targeting of GspD-beta to the outer membrane.

PTM/Processing

Features

Showing features for signal, lipidation, chain, disulfide bond.

TypeIDPosition(s)Description
Signal1-24
Lipidation25N-palmitoyl cysteine
Lipidation25S-diacylglycerol cysteine
ChainPRO_500317970125-136Pilotin AspS 2
Disulfide bond94↔131

Keywords

Interaction

Subunit

Cryo-electron microscopy shows the complex forms a cylindrical channel with 15 GspD2 subunits, each of which interacts with its surroundingy AspS2 (GspS-beta).

Family & Domains

Sequence similarities

Belongs to the GspS/AspS pilotin family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    136
  • Mass (Da)
    14,670
  • Last updated
    2011-01-11 v1
  • Checksum
    8CF2819C427D869A
MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNISQNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLMLTEGINYSITINDTRTGNQYQRKLDRTTCGIVKA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FN649414
EMBL· GenBank· DDBJ
CBJ02739.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp