E3D7E5 · E3D7E5_GARV3
- ProteinLipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD
- GenegatD
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids268 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The GatD subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia. The resulting ammonia molecule is channeled to the active site of MurT.
Catalytic activity
- ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H2O + L-glutamine = ADP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H+ + L-glutamate + phosphate
Pathway
Cell wall biogenesis; peptidoglycan biosynthesis.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 113 | Nucleophile | ||||
Sequence: C | ||||||
Binding site | 147 | substrate | ||||
Sequence: R | ||||||
Active site | 211 | |||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | carbon-nitrogen ligase activity on lipid II | |
Molecular Function | glutaminase activity | |
Biological Process | cell wall organization | |
Biological Process | cobalamin biosynthetic process | |
Biological Process | glutamine metabolic process | |
Biological Process | peptidoglycan biosynthetic process | |
Biological Process | regulation of cell shape |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameLipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Bifidobacteriales > Bifidobacteriaceae > Gardnerella
Accessions
- Primary accessionE3D7E5
Proteomes
Interaction
Subunit
Forms a heterodimer with MurT.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 25-218 | CobB/CobQ-like glutamine amidotransferase | ||||
Sequence: DIVSLYPKDMNIYGDYGNVLTIMRRAELYGYKPVLHEYNVGDDWPKRVDMVLGGGGQDHGQSRVTDDLFARAGAIRELAKNGVPMLMICGLYQLFGEYFETIEGQKLKGIGILGEHTVGQDVRMIGNLVEHSRDFGDIVGYENHSGRTSLESGTQPLGSVDGENCGNNGEDHTEGARKYNVIGTYMHGSVLPKN |
Sequence similarities
Belongs to the CobB/CobQ family. GatD subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length268
- Mass (Da)29,787
- Last updated2011-01-11 v1
- Checksum2D6DB323DDF64C6C
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP002104 EMBL· GenBank· DDBJ | ADP38176.1 EMBL· GenBank· DDBJ | Genomic DNA |