E2QUD0 · GATA_CANLF
- ProteinGlutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial
- GeneQRSL1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids526 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).
Catalytic activity
- ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H+ + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 76 | Charge relay system | ||||
Sequence: K | ||||||
Active site | 171 | Charge relay system | ||||
Sequence: S | ||||||
Active site | 195 | Acyl-ester intermediate | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | glutamyl-tRNA(Gln) amidotransferase complex | |
Cellular Component | mitochondrion | |
Molecular Function | ATP binding | |
Molecular Function | glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity | |
Biological Process | glutaminyl-tRNAGln biosynthesis via transamidation | |
Biological Process | mitochondrial translation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial
- EC number
- Short namesGlu-AdT subunit A
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Carnivora > Caniformia > Canidae > Canis
Accessions
- Primary accessionE2QUD0
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000413331 | 1-526 | Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial | |||
Sequence: MLGRTLREVSAALKQGQITPTELCQKCLSLIKKTKFLNAYITVSEEVALKQAEESEKRYKKGHSLGDLDGIPIAVKDNFSTSGIETTCASNMLKGYVPPYNATVVQKLLDQGAVLMGKTNLDEFAMGSGSTDGVFGPVKNPWSYSKQYKEKTKHKAHGENEDSNWLITGGSSGGSAAAVATFTCFAALGSDTGGSTRNPAAHCGVVGFKPSYGLVSRHGLIPLVNSMDVPGILTRCVDDAAIVLGMLAGHDPKDSTTVQDPVKPFTLPSLTDVSKLCIGIPKEYLTPELSSEVQSLWSKAANLFESEGAKVIEVSLPHTSYSIVCYHVLCTSEVASNMARFDGLEYGHRCDIDVSTEAMYAATRREGFNDVVRGRILSGNFFLLKENYENYFIKAQKVRRLIANDFVNVFNSGVDVLLTPTTLREAVPYQEFIKEDNRTRSAQDDIFTQAVNMAGLPAVSPVALSNQGLPVGLQFIGRAFHDQQLLTVAKWFEKQVQFPVTQLQELMDDCSSVFEIEKLASVSLKQ |
Proteomic databases
Interaction
Subunit
Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (GATB) and C (GATC) subunits.
Protein-protein interaction databases
Structure
Family & Domains
Sequence similarities
Belongs to the amidase family. GatA subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length526
- Mass (Da)57,329
- Last updated2010-11-30 v1
- Checksum4ECFD1177490564B
Keywords
- Technical term