E2QNF8 · E2QNF8_ECOLX

  • Protein
    Protein/nucleic acid deglycase HchA
  • Gene
    hchA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    5/5

Function

function

Protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals. Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of Schiff bases and advanced glycation endproducts (AGE). Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair. Plays an important role in protecting cells from carbonyl stress.

Catalytic activity

  • H2O + N2-(1-hydroxy-2-oxoethyl)-GDP = GDP + glycolate + H+
  • H2O + N2-(1-hydroxy-2-oxoethyl)-GMP = glycolate + GMP + H+
  • H2O + N2-(1-hydroxy-2-oxoethyl)-GTP = glycolate + GTP + H+
  • H2O + N2-(1-hydroxy-2-oxoethyl)-dGTP = dGTP + glycolate + H+
  • H2O + N2-(1-hydroxy-2-oxopropyl)-GDP = GDP + H+ + lactate
  • H2O + N2-(1-hydroxy-2-oxopropyl)-GMP = GMP + H+ + lactate
  • H2O + N2-(1-hydroxy-2-oxopropyl)-GTP = GTP + H+ + lactate
  • H2O + N2-(1-hydroxy-2-oxopropyl)-dGTP = dGTP + H+ + lactate
  • H2O + N6-(1-hydroxy-2-oxoethyl)-L-lysyl-[protein] = glycolate + H+ + L-lysyl-[protein]
  • H2O + N6-(1-hydroxy-2-oxopropyl)-L-lysyl-[protein] = H+ + L-lysyl-[protein] + lactate
    EC:3.5.1.124 (UniProtKB | ENZYME | Rhea)
  • H2O + N(omega)-(1-hydroxy-2-oxoethyl)-L-arginyl-[protein] = glycolate + H+ + L-arginyl-[protein]
  • H2O + N(omega)-(1-hydroxy-2-oxopropyl)-L-arginyl-[protein] = H+ + L-arginyl-[protein] + lactate
    EC:3.5.1.124 (UniProtKB | ENZYME | Rhea)
  • H2O + S-(1-hydroxy-2-oxoethyl)-L-cysteinyl-[protein] = glycolate + H+ + L-cysteinyl-[protein]
  • H2O + S-(1-hydroxy-2-oxopropyl)-L-cysteinyl-[protein] = H+ + L-cysteinyl-[protein] + lactate
    EC:3.5.1.124 (UniProtKB | ENZYME | Rhea)
  • H2O + methylglyoxal = (R)-lactate + H+
    EC:4.2.1.130 (UniProtKB | ENZYME | Rhea)
  • an N2-(1-hydroxy-2-oxoethyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + glycolate + H+
  • an N2-(1-hydroxy-2-oxoethyl)-guanosine in RNA + H2O = a guanosine in RNA + glycolate + H+
  • an N2-(1-hydroxy-2-oxopropyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + H+ + lactate
  • an N2-(1-hydroxy-2-oxopropyl)-guanosine in RNA + H2O = a guanosine in RNA + H+ + lactate

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site86Zn2+ (UniProtKB | ChEBI)
Binding site91Zn2+ (UniProtKB | ChEBI)
Binding site123Zn2+ (UniProtKB | ChEBI)
Active site185Nucleophile

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionlyase activity
Molecular Functionprotein deglycase activity
Molecular Functionthiolester hydrolase activity
Molecular Functionzinc ion binding
Biological ProcessDNA repair
Biological Processprotein repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Protein/nucleic acid deglycase HchA
  • EC number
  • Alternative names
    • Maillard deglycase

Gene names

    • Name
      hchA
    • ORF names
      A2J79_001165
      , A5U30_003607
      , BE932_06040
      , C7B02_06890
      , D3C88_02905
      , D4N09_01015
      , D9E49_19020
      , D9J61_14085
      , EPS76_06485
      , EWK56_00075
      , FPI65_11895
      , GKF66_22765
      , GP711_13090
      , GQN34_00290
      , GRC73_11620
      , HIE29_004084
      , HVV39_22385
      , NCTC7928_05955
      , NCTC9075_02834
      , OGM49_14450
      , PWL68_000643
      , R8O40_003661

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • 93335
    • 1924188
    • Ecol_AZ146
    • S308
    • C0382
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    E2QNF8

Proteomes

Subcellular Location

Keywords

Expression

Induction

By heat shock.

Interaction

Subunit

Homodimer.

Family & Domains

Sequence similarities

Belongs to the peptidase C56 family. HSP31-like subfamily.
Belongs to the peptidase C56 family. HchA subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    283
  • Mass (Da)
    31,236
  • Last updated
    2010-11-30 v1
  • Checksum
    5598412CA61821B2
MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTPDIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQEMLAAYAS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP018991
EMBL· GenBank· DDBJ
AQV72058.1
EMBL· GenBank· DDBJ
Genomic DNA
AAAGZE010000033
EMBL· GenBank· DDBJ
EAC1533151.1
EMBL· GenBank· DDBJ
Genomic DNA
AATCLQ010000005
EMBL· GenBank· DDBJ
EFJ6480834.1
EMBL· GenBank· DDBJ
Genomic DNA
AATLZG010000025
EMBL· GenBank· DDBJ
EFM8155929.1
EMBL· GenBank· DDBJ
Genomic DNA
ABONVU020000015
EMBL· GenBank· DDBJ
EMJ5255381.1
EMBL· GenBank· DDBJ
Genomic DNA
ABKSHZ030000001
EMBL· GenBank· DDBJ
EMM9720616.1
EMBL· GenBank· DDBJ
Genomic DNA
DABBJX010000011
EMBL· GenBank· DDBJ
HAH4524656.1
EMBL· GenBank· DDBJ
Genomic DNA
DABCJL010000011
EMBL· GenBank· DDBJ
HAH7770598.1
EMBL· GenBank· DDBJ
Genomic DNA
WSGM01000006
EMBL· GenBank· DDBJ
KAE9731604.1
EMBL· GenBank· DDBJ
Genomic DNA
ROAL01000019
EMBL· GenBank· DDBJ
MIB62452.1
EMBL· GenBank· DDBJ
Genomic DNA
WKUE01000056
EMBL· GenBank· DDBJ
MSI71568.1
EMBL· GenBank· DDBJ
Genomic DNA
WTOC01000001
EMBL· GenBank· DDBJ
MWP22525.1
EMBL· GenBank· DDBJ
Genomic DNA
VLTB01000200
EMBL· GenBank· DDBJ
NDR91969.1
EMBL· GenBank· DDBJ
Genomic DNA
PYRA01000005
EMBL· GenBank· DDBJ
PSY95301.1
EMBL· GenBank· DDBJ
Genomic DNA
CP055981
EMBL· GenBank· DDBJ
QMS40562.1
EMBL· GenBank· DDBJ
Genomic DNA
QXHA01000167
EMBL· GenBank· DDBJ
RIB43405.1
EMBL· GenBank· DDBJ
Genomic DNA
SCJN01000032
EMBL· GenBank· DDBJ
RXD17210.1
EMBL· GenBank· DDBJ
Genomic DNA
SERV01000001
EMBL· GenBank· DDBJ
RYL85614.1
EMBL· GenBank· DDBJ
Genomic DNA
UGAB01000002
EMBL· GenBank· DDBJ
STF45188.1
EMBL· GenBank· DDBJ
Genomic DNA
UGEM01000004
EMBL· GenBank· DDBJ
STP19416.1
EMBL· GenBank· DDBJ
Genomic DNA
QYOH01000001
EMBL· GenBank· DDBJ
TXU38084.1
EMBL· GenBank· DDBJ
Genomic DNA
CP107128
EMBL· GenBank· DDBJ
WLM93944.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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