E2QDB5 · E2QDB5_PHACH
- ProteinNADH-cytochrome b5 reductase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids321 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
Catalytic activity
- 2 Fe3+-[Dph3] + NADH = 2 Fe2+-[Dph3] + H+ + NAD+This reaction proceeds in the forward direction.
Cofactor
Pathway
Protein modification; peptidyl-diphthamide biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 154 | FAD (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 156 | FAD (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 171 | FAD (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 173 | FAD (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 180 | FAD (UniProtKB | ChEBI) | ||||
Sequence: I | ||||||
Binding site | 181 | FAD (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 222 | FAD (UniProtKB | ChEBI) | ||||
Sequence: T |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Cellular Component | mitochondrion | |
Molecular Function | 2-(3-amino-3-carboxypropyl)histidine synthase activity | |
Molecular Function | cytochrome-b5 reductase activity, acting on NAD(P)H | |
Molecular Function | NADH dehydrogenase activity | |
Biological Process | protein histidyl modification to diphthamide | |
Biological Process | tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADH-cytochrome b5 reductase
- EC number
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Basidiomycota > Agaricomycotina > Agaricomycetes > Polyporales > Phanerochaetaceae > Phanerodontia
Accessions
- Primary accessionE2QDB5
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 7-27 | Helical | ||||
Sequence: LAFIASFLGVFSGLVLVSNFV | ||||||
Transmembrane | 70-89 | Helical | ||||
Sequence: MFAQLLALILAALTSAAIYY |
Keywords
- Cellular component
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 102-205 | FAD-binding FR-type | ||||
Sequence: GKWQEFPLKEKIVISPNTAIYRFALPHPQDVLGLPIGQHISVQAEINGKDIMRSYTPTSSDDDLGHFDLLIKAYEKGNISRYISLLKIGDKIRVKGPKGQFNYS |
Sequence similarities
Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length321
- Mass (Da)35,520
- Last updated2010-11-30 v1
- ChecksumBFBB4F9CD09BE151
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY835609 EMBL· GenBank· DDBJ | AAX49402.1 EMBL· GenBank· DDBJ | mRNA |