E2Q5Q4 · E2Q5Q4_STRCL

  • Protein
    Arginine biosynthesis bifunctional protein ArgJ
  • Gene
    oat2
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N2-acetylornithine and glutamate.

Miscellaneous

Some bacteria possess a monofunctional ArgJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N2-acetyl-L-ornithine (cyclic): step 1/1.
Amino-acid biosynthesis; L-arginine biosynthesis; N2-acetyl-L-ornithine from L-glutamate: step 1/4.

Features

Showing features for site, binding site, active site.

Type
IDPosition(s)Description
Site117Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole
Site118Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole
Binding site154substrate
Binding site176substrate
Site186-187Cleavage; by autolysis
Active site187Nucleophile
Binding site187substrate
Binding site266substrate
Binding site394substrate
Binding site399substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionglutamate N-acetyltransferase activity
Molecular FunctionL-glutamate N-acetyltransferase activity
Biological Processarginine biosynthetic process
Biological Processornithine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

Including 2 domains:

  • Recommended name
    Glutamate N-acetyltransferase
  • EC number
  • Alternative names
    • Ornithine acetyltransferase
      (OATase
      )
    • Ornithine transacetylase
  • Recommended name
    Amino-acid acetyltransferase
  • EC number
  • Alternative names
    • N-acetylglutamate synthase
      (AGSase
      )

Gene names

    • Name
      oat2
    • Synonyms
      argJ
    • ORF names
      SCLAV_4193

Organism names

  • Taxonomic identifier
  • Strain
    • ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces

Accessions

  • Primary accession
    E2Q5Q4

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_50235661771-186Arginine biosynthesis bifunctional protein ArgJ alpha chain
ChainPRO_5023566176187-399Arginine biosynthesis bifunctional protein ArgJ beta chain

Keywords

Interaction

Subunit

Heterotetramer of two alpha and two beta chains.

Family & Domains

Sequence similarities

Belongs to the ArgJ family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    399
  • Mass (Da)
    42,198
  • Last updated
    2010-11-30 v1
  • Checksum
    4A8F3F90BE5C744F
MTGDADMSDSTPKTPRGFVVHTAPVGLADDGRDDFTVLASTAPATVSAVFTRSRFAGPSVVLCREAVADGQARGVVVLARNANVATGLEGEENAREVREAVARALGLPEGEMLIASTGVIGRQYPMESIREHLKTLEWPAGEGGFDRAARAIMTTDTRPKEVRVSVGGATLVGIAKGVGMLEPDMATLLTFFATDARLDPAEQDRLFRRVMDRTFNAVSIDTDTSTSDTAVLFANGLAGEVDAGEFEEALHTAALALVKDIASDGEGAAKLIEVQVTGARDDAQAKRVGKTVVNSPLVKTAVHGCDPNWGRVAMAIGKCSDDTDIDQERVTIRFGEVEVYPPKARGDQADDALRAAVAEHLRGDEVVIGIDLAIADGAFTVYGCDLTEGYVRLNSEYTT

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CM000913
EMBL· GenBank· DDBJ
EFG09268.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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