E1ZZH4 · E1ZZH4_CAMFO
- ProteinmRNA-capping enzyme
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids591 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphate monophosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the GMP moiety of GTP to the 5'-diphosphate terminus of RNA via a covalent enzyme-GMP reaction intermediate.
Catalytic activity
- a 5'-end diphospho-ribonucleoside in mRNA + GTP + H+ = a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + diphosphateThis reaction proceeds in the forward direction.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 130 | Phosphocysteine intermediate | ||||
Sequence: C | ||||||
Active site | 299 | N6-GMP-lysine intermediate | ||||
Sequence: K | ||||||
Binding site | 304 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 320 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 348-350 | GTP (UniProtKB | ChEBI) | ||||
Sequence: DGE | ||||||
Binding site | 465-467 | GTP (UniProtKB | ChEBI) | ||||
Sequence: KWK | ||||||
Binding site | 536-541 | GTP (UniProtKB | ChEBI) | ||||
Sequence: RQRVDK |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | GTP binding | |
Molecular Function | mRNA 5'-triphosphate monophosphatase activity | |
Molecular Function | mRNA guanylyltransferase activity | |
Molecular Function | polynucleotide 5'-phosphatase activity | |
Molecular Function | protein tyrosine/serine/threonine phosphatase activity | |
Biological Process | 7-methylguanosine mRNA capping |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended namemRNA-capping enzyme
Including 2 domains:
- Recommended namemRNA 5'-triphosphate monophosphatase
- EC number
- Alternative names
- Recommended namemRNA guanylyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Hymenoptera > Apocrita > Aculeata > Formicoidea > Formicidae > Formicinae > Camponotus
Accessions
- Primary accessionE1ZZH4
Proteomes
Subcellular Location
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 39-187 | Tyrosine-protein phosphatase | ||||
Sequence: YDNQVPEECRFSVDMFFANLKSQKIKIGLWIDLTNTTRFYDRKTVEDYDCKYLKLQCRGHGETPSEEQTRTFVQVCRNFIAHNPLEIIGVHCTHGFNRTGFLIISYLLEIDGSSVDAALAEFATVRPPGIYKDDYIKELYRRYDDMDDA | ||||||
Domain | 108-175 | Tyrosine specific protein phosphatases | ||||
Sequence: RTFVQVCRNFIAHNPLEIIGVHCTHGFNRTGFLIISYLLEIDGSSVDAALAEFATVRPPGIYKDDYIK | ||||||
Region | 207-233 | Disordered | ||||
Sequence: DVDEGPSTENENCHEEGQGKKRRREFN | ||||||
Compositional bias | 213-233 | Basic and acidic residues | ||||
Sequence: STENENCHEEGQGKKRRREFN |
Sequence similarities
In the C-terminal section; belongs to the eukaryotic GTase family.
In the N-terminal section; belongs to the non-receptor class of the protein-tyrosine phosphatase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length591
- Mass (Da)68,186
- Last updated2010-11-30 v1
- Checksum2E54368374F4F84F
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 213-233 | Basic and acidic residues | ||||
Sequence: STENENCHEEGQGKKRRREFN |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
GL435347 EMBL· GenBank· DDBJ | EFN73425.1 EMBL· GenBank· DDBJ | Genomic DNA |