E1BF91 · E1BF91_BOVIN

  • Protein
    Alpha-tubulin N-acetyltransferase 1
  • Gene
    ATAT1
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    5/5

Function

function

Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.

Catalytic activity

Features

Showing features for site, binding site.

137750100150200250300350
Type
IDPosition(s)Description
Site102Crucial for catalytic activity
Binding site168-181acetyl-CoA (UniProtKB | ChEBI)
Binding site204-213acetyl-CoA (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentaxon
Cellular Componentclathrin-coated pit
Cellular Componentcytosol
Cellular Componentfocal adhesion
Cellular ComponentGolgi apparatus
Cellular Componentmicrotubule
Cellular Componentmicrotubule bundle
Cellular Componentmitotic spindle
Molecular Functionlysine N-acetyltransferase activity, acting on acetyl phosphate as donor
Molecular Functiontubulin N-acetyltransferase activity
Biological Processalpha-tubulin acetylation
Biological Processdentate gyrus development
Biological Processmicrotubule cytoskeleton organization
Biological Processneuron development
Biological ProcessNLRP3 inflammasome complex assembly
Biological Processpositive regulation of NLRP3 inflammasome complex assembly
Biological Processregulation of fat cell differentiation
Biological Processregulation of microtubule cytoskeleton organization
Biological Processresponse to mechanical stimulus
Biological Processresponse to pain
Biological Processspermatogenesis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Alpha-tubulin N-acetyltransferase 1
  • EC number
  • Short names
    Alpha-TAT
    ; Alpha-TAT1
    ; TAT
  • Alternative names
    • Acetyltransferase mec-17 homolog

Gene names

    • Name
      ATAT1
    • Synonyms
      MEC17

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Hereford
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos

Accessions

  • Primary accession
    E1BF91

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue100N6-acetyllysine; by autocatalysis
Modified residue190N6-acetyllysine; by autocatalysis

Post-translational modification

Autoacetylation strongly increases tubulin acetylation.

Keywords

Expression

Gene expression databases

Interaction

Subunit

Component of the BBSome complex. Interacts with AP2 alpha-adaptins, including AP2A2, but not with AP1 gamma-adaptin (AP1G1/AP1G2); this interaction is required for efficient alpha-tubulin acetylation, hence clathrin-coated pits are sites of microtubule acetylation.

Structure

3D structure databases

Family & Domains

Features

Showing features for region, domain, compositional bias.

Type
IDPosition(s)Description
Region1-36Disordered
Domain45-234N-acetyltransferase
Region241-283Disordered
Compositional bias268-282Basic and acidic residues
Region296-331Disordered
Compositional bias299-314Pro residues
Region347-377Disordered

Sequence similarities

Belongs to the acetyltransferase ATAT1 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    377
  • Mass (Da)
    41,908
  • Last updated
    2024-05-29 v3
  • Checksum
    613D68F2F34C48CD
MVEEGSAGRGGPLGTCRRGRGNTDCGGRGGSDVARGGSDRAQWAMEFPFDVDALLPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIVDELGKASAKAQHLPAPITSASRMQSNRHVMYILKDTSARPAGKGAIVGFLKVGYKKLFVLDDREAHNEVEPLCILDFYIHESLQRHGHGRELFQHMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHRPPATSLRATRHSRAAALDPMPAAPARKLPPKRAEGDIKPYSSSDREFLKVAVEPPWPLNRAPRRATPPAHPPPRSSSLGNSPERGPLRPFVPEQELLRSLRLCPPHPTARLLLATDPGGSPAQRRRTSSLPRSDESRY

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias268-282Basic and acidic residues
Compositional bias299-314Pro residues

Keywords

Genome annotation databases

Similar Proteins

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