E0UWS9 · E0UWS9_9INFA

Function

function

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.

GO annotations

AspectTerm
Cellular Componenthelical viral capsid
Cellular Componenthost cell
Cellular Componenthost cell nucleus
Cellular Componentribonucleoprotein complex
Cellular Componentviral nucleocapsid
Molecular FunctionRNA binding
Molecular Functionstructural molecule activity
Biological Processsymbiont entry into host cell
Biological Processviral penetration into host nucleus

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Nucleoprotein
  • Alternative names
    • Nucleocapsid protein
      (Protein N
      )

Gene names

    • Name
      NP

Organism names

Accessions

  • Primary accession
    E0UWS9

Proteomes

Subcellular Location

Keywords

PTM/Processing

Post-translational modification

Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction.

Interaction

Subunit

Homomultimerizes to form the nucleocapsid. May bind host exportin-1/XPO1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases.

Family & Domains

Features

Showing features for motif, region.

Type
IDPosition(s)Description
Motif1-18Unconventional nuclear localization signal
Region1-21Disordered
Motif198-216Bipartite nuclear localization signal

Sequence similarities

Belongs to the influenza viruses nucleoprotein family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    498
  • Mass (Da)
    56,237
  • Last updated
    2010-11-02 v1
  • Checksum
    55FAB72D968D8934
MASQGTKRSYEQMETGGERQNATEIRASVGRMVGGIGRFYIQMCTELKLSDHEGRLIQNSITIERMVLSAFDERRNKYLEEHPSAGKDPKKTGGPIYRRRDGKWIRELILYDKEEIRRIWRQANNGEDATAGLTHMMIWHSNLNDTTYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTMVMELIRMIKRGINDRNFWRGENGRRTRIAYERMCNILKGKFQTAAQRAMMDQVRESRNPGNAEIEDLIFLARSALILRGSVAHKSCLPACVYGLAVASGYDFEREGYSLVGIDPFKLLQNSQVFSLIRPNENPAHKSQLVWMACHSAAFEDLRVSSFIRGTRVVPRGQLATRGVQIASNENMETIDSSTLELRSRYWAIRTRSGGNTNQQRASAGQISVQPTFSVQRNLPFERATIMAAFTGNTEGRTSDMRTEIIRMMENARPEDVSFQGRGVFELSDEKATNPIVPSFDMSNEGSYFFGDNAEEFDN

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CY067695
EMBL· GenBank· DDBJ
ADK78186.1
EMBL· GenBank· DDBJ
Viral cRNA

Similar Proteins

Disclaimer

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