E0UWS9 · E0UWS9_9INFA
- ProteinNucleoprotein
- GeneNP
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids498 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | helical viral capsid | |
Cellular Component | host cell | |
Cellular Component | host cell nucleus | |
Cellular Component | ribonucleoprotein complex | |
Cellular Component | viral nucleocapsid | |
Molecular Function | RNA binding | |
Molecular Function | structural molecule activity | |
Biological Process | symbiont entry into host cell | |
Biological Process | viral penetration into host nucleus |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameNucleoprotein
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Negarnaviricota > Polyploviricotina > Insthoviricetes > Articulavirales > Orthomyxoviridae > Alphainfluenzavirus > Alphainfluenzavirus influenzae > Influenza A virus
Accessions
- Primary accessionE0UWS9
Proteomes
Subcellular Location
PTM/Processing
Post-translational modification
Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction.
Interaction
Subunit
Homomultimerizes to form the nucleocapsid. May bind host exportin-1/XPO1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases.
Structure
Family & Domains
Features
Showing features for motif, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Motif | 1-18 | Unconventional nuclear localization signal | |||
Region | 1-21 | Disordered | |||
Motif | 198-216 | Bipartite nuclear localization signal | |||
Sequence similarities
Belongs to the influenza viruses nucleoprotein family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length498
- Mass (Da)56,237
- Last updated2010-11-02 v1
- Checksum55FAB72D968D8934
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CY067695 EMBL· GenBank· DDBJ | ADK78186.1 EMBL· GenBank· DDBJ | Viral cRNA |