E0N6I8 · E0N6I8_NEIME
- ProteintRNA-dihydrouridine(20/20a) synthase
- GenedusA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids348 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs.
Catalytic activity
- 5,6-dihydrouridine20 in tRNA + NAD+ = H+ + NADH + uridine20 in tRNA
- 5,6-dihydrouridine(20a) in tRNA + NAD+ = H+ + NADH + uridine(20a) in tRNA
- 5,6-dihydrouridine(20a) in tRNA + NADP+ = H+ + NADPH + uridine(20a) in tRNA
Cofactor
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 29-31 | FMN (UniProtKB | ChEBI) | ||||
Sequence: PML | ||||||
Binding site | 82 | FMN (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Site | 109 | Interacts with tRNA | ||||
Sequence: N | ||||||
Active site | 112 | Proton donor | ||||
Sequence: C | ||||||
Binding site | 151 | FMN (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 184 | FMN (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Site | 196 | Interacts with tRNA; defines subfamily-specific binding signature | ||||
Sequence: K | ||||||
Site | 199 | Interacts with tRNA | ||||
Sequence: R | ||||||
Binding site | 224-226 | FMN (UniProtKB | ChEBI) | ||||
Sequence: NGG | ||||||
Binding site | 246-247 | FMN (UniProtKB | ChEBI) | ||||
Sequence: GR | ||||||
Site | 314 | Interacts with tRNA; defines subfamily-specific binding signature | ||||
Sequence: R | ||||||
Site | 317 | Interacts with tRNA; defines subfamily-specific binding signature | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | FMN binding | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-dihydrouridine20 synthase activity | |
Molecular Function | tRNA-dihydrouridine20a synthase activity |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-dihydrouridine(20/20a) synthase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Neisseriales > Neisseriaceae > Neisseria
Accessions
- Primary accessionE0N6I8
Proteomes
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 27-329 | DUS-like FMN-binding | ||||
Sequence: VAPMLDWTDRHYRYMARQITRNAWLYSEMVNAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGCPSPRVQKGSFGACLMNEVGLVADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTLRDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPELEIIINGGITANEAIAGHLQHVDGVMVGREAYHNPIVMREWDRLFYGDTRSPIEYTDLVQRLYTYSQAQIQAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDN |
Sequence similarities
Belongs to the Dus family. DusA subfamily.
Belongs to the dus family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length348
- Mass (Da)39,567
- Last updated2010-11-02 v1
- Checksum68DDFBE67DCA256B