E0N6I8 · E0N6I8_NEIME

Function

function

Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

FMN (UniProtKB | Rhea| CHEBI:58210 )

Features

Showing features for binding site, site, active site.

TypeIDPosition(s)Description
Binding site29-31FMN (UniProtKB | ChEBI)
Binding site82FMN (UniProtKB | ChEBI)
Site109Interacts with tRNA
Active site112Proton donor
Binding site151FMN (UniProtKB | ChEBI)
Binding site184FMN (UniProtKB | ChEBI)
Site196Interacts with tRNA; defines subfamily-specific binding signature
Site199Interacts with tRNA
Binding site224-226FMN (UniProtKB | ChEBI)
Binding site246-247FMN (UniProtKB | ChEBI)
Site314Interacts with tRNA; defines subfamily-specific binding signature
Site317Interacts with tRNA; defines subfamily-specific binding signature

GO annotations

AspectTerm
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionFMN binding
Molecular FunctiontRNA binding
Molecular FunctiontRNA-dihydrouridine20 synthase activity
Molecular FunctiontRNA-dihydrouridine20a synthase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-dihydrouridine(20/20a) synthase
  • EC number
  • Alternative names
    • U20-specific dihydrouridine synthase
      (U20-specific Dus
      )
    • tRNA-dihydrouridine synthase A

Gene names

    • Name
      dusA
    • ORF names
      HMPREF0602_0118

Organism names

Accessions

  • Primary accession
    E0N6I8

Proteomes

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain27-329DUS-like FMN-binding

Sequence similarities

Belongs to the Dus family. DusA subfamily.
Belongs to the dus family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    348
  • Mass (Da)
    39,567
  • Last updated
    2010-11-02 v1
  • Checksum
    68DDFBE67DCA256B
MFELKECENLMINKELIEHKGKRRLCVAPMLDWTDRHYRYMARQITRNAWLYSEMVNAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGCPSPRVQKGSFGACLMNEVGLVADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTLRDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPELEIIINGGITANEAIAGHLQHVDGVMVGREAYHNPIVMREWDRLFYGDTRSPIEYTDLVQRLYTYSQAQIQAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANTRE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AEEF01000001
EMBL· GenBank· DDBJ
EFM05496.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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