D9QA09 · D9QA09_CORP2

Function

function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
[2Fe-2S] cluster (UniProtKB | Rhea| CHEBI:190135 )

Note: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.
[2Fe-2S] cluster (UniProtKB | Rhea| CHEBI:190135 )

Note: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.
[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site66[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site70[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site73[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site111[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site143[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site203[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site273[2Fe-2S] cluster (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function2 iron, 2 sulfur cluster binding
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionbiotin synthase activity
Molecular Functioniron ion binding
Biological Processbiotin biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Biotin synthase
  • EC number

Gene names

    • Name
      bioB
    • ORF names
      CPC231_04565

Organism names

Accessions

  • Primary accession
    D9QA09

Proteomes

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain48-278Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    334
  • Mass (Da)
    36,226
  • Last updated
    2010-10-05 v1
  • Checksum
    5B99705A8CCC410F
MTTTLNDVQNLKERVLAGEIIDRATALALHDAPLDELAQAADDIRQHFCGNGFDMCSIINAKSGRCPENCTFCAQSIRFPNIEIDKYPLISVDELLAQAKENKEKGVLRFSIVTSGRKLRRDEVEQICEGVRRIRAEIGIEVCISAGLLTEEQFTLLHNTGVSRVHCNLETSRNNFPNICTSHTFEDKIETLEAAKRAGMSLCSGGILGLGETFEDRIDMVISARELGVKSIPVNVLVAIEGTPLQGMVPVPPEEVRRAVAIFRFLCPDAAIRLAGGRELLGDHGEACFTSGANSAISGDMLTTTGSTIASDLTLVKELGFDVALDHDDRAVNG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001829
EMBL· GenBank· DDBJ
ADL10385.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp