D9Q064 · D9Q064_ACIS3
- ProteinProteasome-activating nucleotidase
- Genepan
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids388 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
ATPase which is responsible for recognizing, binding, unfolding and translocation of substrate proteins into the archaeal 20S proteasome core particle. Is essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Unfolding activity requires energy from ATP hydrolysis, whereas ATP binding alone promotes ATPase-20S proteasome association which triggers gate opening, and supports translocation of unfolded substrates.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 177-182 | ATP (UniProtKB | ChEBI) | |||
Binding site | 316 | ATP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | proteasome regulatory particle, base subcomplex | |
Cellular Component | proteasome-activating nucleotidase complex | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | proteasome-activating activity | |
Biological Process | proteasome-mediated ubiquitin-dependent protein catabolic process | |
Biological Process | protein unfolding |
Keywords
- Molecular function
- Ligand
Names & Taxonomy
Protein names
- Recommended nameProteasome-activating nucleotidase
- Short namesPAN
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Thermoproteota > Thermoprotei > Acidilobales > Acidilobaceae > Acidilobus
Accessions
- Primary accessionD9Q064
Proteomes
Subcellular Location
Interaction
Subunit
Homohexamer. The hexameric complex has a two-ring architecture resembling a top hat that caps the 20S proteasome core at one or both ends. Upon ATP-binding, the C-terminus of PAN interacts with the alpha-rings of the proteasome core by binding to the intersubunit pockets.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 166-305 | AAA+ ATPase | |||
Domain
Consists of three main regions, an N-terminal coiled-coil domain that may assist in substrate recognition, an interdomain involved in PAN hexamerization, and a C-terminal ATPase domain of the AAA type.
Sequence similarities
Belongs to the AAA ATPase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length388
- Mass (Da)43,325
- Last updated2010-10-05 v1
- ChecksumD9565B32BD868432
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001742 EMBL· GenBank· DDBJ | ADL18702.1 EMBL· GenBank· DDBJ | Genomic DNA |