D9Q026 · D9Q026_ACIS3
- ProteinS-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
- Genetaw1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids347 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Component of the wyosine derivatives biosynthesis pathway that catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine (imG-14) on guanosine-37 of tRNA(Phe).
Catalytic activity
- N1-methylguanosine37 in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine37 in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O
RHEA-COMP:10165 CHEBI:73542 Position: 37+ CHEBI:15361 + CHEBI:59789 = RHEA-COMP:10164 CHEBI:64315 Position: 37+ CHEBI:17319 + CHEBI:57844 + CHEBI:16526 + CHEBI:15377
Cofactor
Note: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 36 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 49 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 62 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 72 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 76 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 79 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | tRNA-4-demethylwyosine synthase activity | |
Biological Process | tRNA processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameS-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Thermoproteota > Thermoprotei > Acidilobales > Acidilobaceae > Acidilobus
Accessions
- Primary accessionD9Q026
Proteomes
Subcellular Location
Interaction
Structure
Sequence
- Sequence statusComplete
- Length347
- Mass (Da)40,289
- Last updated2010-10-05 v1
- MD5 Checksum8E9BC063021F12D9598F96EAFE55500F
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001742 EMBL· GenBank· DDBJ | ADL18664.1 EMBL· GenBank· DDBJ | Genomic DNA |