D9PVI7 · MDH_METTM
- ProteinMalate dehydrogenase
- Genemdh
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids325 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Catalyzes the reversible oxidation of malate to oxaloacetate. Can use NAD+ and NADP+ with similar specific activity.
Catalytic activity
- (S)-malate + NADP+ = H+ + NADPH + oxaloacetate
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.03 mM | oxaloacetate | |||||
0.09 mM | NADH | |||||
0.02 mM | NADPH | |||||
1 mM | malate | |||||
0.5 mM | NAD+ |
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 7-13 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: GSTGRVG | ||||||
Binding site | 84 | substrate | ||||
Sequence: R | ||||||
Binding site | 90 | substrate | ||||
Sequence: R | ||||||
Binding site | 97 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 120-122 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: VTN | ||||||
Binding site | 122 | substrate | ||||
Sequence: N | ||||||
Binding site | 153 | substrate | ||||
Sequence: R | ||||||
Active site | 177 | Proton acceptor | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | L-malate dehydrogenase activity | |
Molecular Function | malate dehydrogenase (NADP+) activity | |
Biological Process | carboxylic acid metabolic process | |
Biological Process | tricarboxylic acid cycle |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMalate dehydrogenase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Methanomada group > Methanobacteria > Methanobacteriales > Methanobacteriaceae > Methanothermobacter
Accessions
- Primary accessionD9PVI7
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000436035 | 1-325 | Malate dehydrogenase | |||
Sequence: MKVSIIGSTGRVGRATALCLAEEEAVKTLHLISRRESLEQNIGEVLDMSDALAAKGVSVKLENSADIENVHGSRIVVITAGVPRTADMDRDDLAFQNGVIVAEYARQIARFAPDSIILVVTNPVDVMTYVALKYSGFHPSRVFGLGNHLDSLRLKNYMARHFNVHVSEVHTRVIGQHGPYMVPLISSTSIGGIPIEHYARRDYFSGYRRFDLKKTIEKVINAGSNIISRKGATEYGPAFAISNIVTTILNDERRILTVSTLMEGEIDGIRDVCLGVPVKLGKNGIEGVVPVLMDRDERETFREAASHVRNSTMKVMEFLDEELPL |
Proteomic databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length325
- Mass (Da)35,860
- Last updated2010-10-05 v1
- Checksum1D4BB57775D4A236
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 1 | in Ref. 2; AA sequence | ||||
Sequence: M → H | ||||||
Sequence conflict | 19 | in Ref. 2; AA sequence | ||||
Sequence: Missing |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001710 EMBL· GenBank· DDBJ | ADL58235.1 EMBL· GenBank· DDBJ | Genomic DNA |