D8ADB5 · MAND_ECOMS
- ProteinD-galactonate dehydratase family member RspA
- GenerspA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids415 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Has low D-mannonate dehydratase activity (in vitro), suggesting that this is not a physiological substrate and that it has no significant role in D-mannonate degradation in vivo. Has no detectable activity with a panel of 70 other acid sugars (in vitro).
Catalytic activity
- D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Kinetics
kcat is 0.01 sec-1 with D-mannonate.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 48 | substrate | ||||
Sequence: N | ||||||
Binding site | 133 | substrate | ||||
Sequence: H | ||||||
Active site | 170 | Proton donor/acceptor | ||||
Sequence: Y | ||||||
Binding site | 223 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Active site | 225 | Proton donor/acceptor | ||||
Sequence: H | ||||||
Binding site | 249 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 275 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 275 | substrate | ||||
Sequence: E | ||||||
Binding site | 296 | substrate | ||||
Sequence: R | ||||||
Binding site | 325 | substrate | ||||
Sequence: H | ||||||
Site | 327 | Important for activity and substrate specificity; Pro is observed in family members with low D-mannonate dehydratase activity | ||||
Sequence: P | ||||||
Binding site | 329 | substrate | ||||
Sequence: D | ||||||
Binding site | 352 | substrate | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | magnesium ion binding | |
Molecular Function | mannonate dehydratase activity | |
Biological Process | amino acid catabolic process | |
Biological Process | carbohydrate catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameD-galactonate dehydratase family member RspA
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionD8ADB5
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000429896 | 1-415 | D-galactonate dehydratase family member RspA | |||
Sequence: MHHDKRCKESNMKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVASYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYGMSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLTPIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHSGDKPGLGIEFDEKLAAKYPYEPAYLPVARLEDGTLWNW |
Structure
Sequence
- Sequence statusComplete
- Length415
- Mass (Da)47,325
- Last updated2010-10-05 v1
- Checksum6AEA02C4CEF7C29A