D8ADB5 · MAND_ECOMS

Function

function

Has low D-mannonate dehydratase activity (in vitro), suggesting that this is not a physiological substrate and that it has no significant role in D-mannonate degradation in vivo. Has no detectable activity with a panel of 70 other acid sugars (in vitro).

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.

Kinetics

kcat is 0.01 sec-1 with D-mannonate.

Features

Showing features for binding site, active site, site.

TypeIDPosition(s)Description
Binding site48substrate
Binding site133substrate
Active site170Proton donor/acceptor
Binding site223Mg2+ (UniProtKB | ChEBI)
Active site225Proton donor/acceptor
Binding site249Mg2+ (UniProtKB | ChEBI)
Binding site275Mg2+ (UniProtKB | ChEBI)
Binding site275substrate
Binding site296substrate
Binding site325substrate
Site327Important for activity and substrate specificity; Pro is observed in family members with low D-mannonate dehydratase activity
Binding site329substrate
Binding site352substrate

GO annotations

AspectTerm
Molecular Functionmagnesium ion binding
Molecular Functionmannonate dehydratase activity
Biological Processamino acid catabolic process
Biological Processcarbohydrate catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    D-galactonate dehydratase family member RspA
  • EC number
  • Alternative names
    • D-mannonate dehydratase (EC:4.2.1.8) . EC:4.2.1.8 (UniProtKB | ENZYME | Rhea)
    • Starvation sensing protein RspA homolog

Gene names

    • Name
      rspA
    • ORF names
      HMPREF9530_04559

Organism names

Accessions

  • Primary accession
    D8ADB5

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004298961-415D-galactonate dehydratase family member RspA

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    415
  • Mass (Da)
    47,325
  • Last updated
    2010-10-05 v1
  • Checksum
    6AEA02C4CEF7C29A
MHHDKRCKESNMKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVASYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYGMSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLTPIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHSGDKPGLGIEFDEKLAAKYPYEPAYLPVARLEDGTLWNW

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
ADTR01000410
EMBL· GenBank· DDBJ
EFK18877.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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